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Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017
Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032538/ https://www.ncbi.nlm.nih.gov/pubmed/33868410 http://dx.doi.org/10.1155/2021/8817987 |
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author | Dieng, Idrissa Diarra, Maryam Diagne, Moussa Moïse Faye, Martin Dior Ndione, Marie Henriette Ba, Yamar Diop, Mamadou Ndiaye, El Hadji Marinho de Andrade Zanotto, Paolo Diop, Boly Ndiaye, Mamadou Bousso, Abdoulaye Dia, Ndongo Diallo, Mawlouth Barry, Aliou Fall, Gamou Loucoubar, Cheikh Sall, Amadou Alpha Faye, Ousmane Faye, Oumar |
author_facet | Dieng, Idrissa Diarra, Maryam Diagne, Moussa Moïse Faye, Martin Dior Ndione, Marie Henriette Ba, Yamar Diop, Mamadou Ndiaye, El Hadji Marinho de Andrade Zanotto, Paolo Diop, Boly Ndiaye, Mamadou Bousso, Abdoulaye Dia, Ndongo Diallo, Mawlouth Barry, Aliou Fall, Gamou Loucoubar, Cheikh Sall, Amadou Alpha Faye, Ousmane Faye, Oumar |
author_sort | Dieng, Idrissa |
collection | PubMed |
description | Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management. |
format | Online Article Text |
id | pubmed-8032538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-80325382021-04-16 Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 Dieng, Idrissa Diarra, Maryam Diagne, Moussa Moïse Faye, Martin Dior Ndione, Marie Henriette Ba, Yamar Diop, Mamadou Ndiaye, El Hadji Marinho de Andrade Zanotto, Paolo Diop, Boly Ndiaye, Mamadou Bousso, Abdoulaye Dia, Ndongo Diallo, Mawlouth Barry, Aliou Fall, Gamou Loucoubar, Cheikh Sall, Amadou Alpha Faye, Ousmane Faye, Oumar J Trop Med Research Article Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management. Hindawi 2021-03-31 /pmc/articles/PMC8032538/ /pubmed/33868410 http://dx.doi.org/10.1155/2021/8817987 Text en Copyright © 2021 Idrissa Dieng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dieng, Idrissa Diarra, Maryam Diagne, Moussa Moïse Faye, Martin Dior Ndione, Marie Henriette Ba, Yamar Diop, Mamadou Ndiaye, El Hadji Marinho de Andrade Zanotto, Paolo Diop, Boly Ndiaye, Mamadou Bousso, Abdoulaye Dia, Ndongo Diallo, Mawlouth Barry, Aliou Fall, Gamou Loucoubar, Cheikh Sall, Amadou Alpha Faye, Ousmane Faye, Oumar Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
title | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
title_full | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
title_fullStr | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
title_full_unstemmed | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
title_short | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
title_sort | field deployment of a mobile biosafety laboratory reveals the co-circulation of dengue viruses serotype 1 and serotype 2 in louga city, senegal, 2017 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032538/ https://www.ncbi.nlm.nih.gov/pubmed/33868410 http://dx.doi.org/10.1155/2021/8817987 |
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