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Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance
DNA methylation plays a crucial role in the regulation of gene expression, activity of transposable elements, defense against foreign DNA, and inheritance of specific gene expression patterns. The link between stress exposure and sequence-specific changes in DNA methylation was hypothetical until it...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032638/ https://www.ncbi.nlm.nih.gov/pubmed/33512663 http://dx.doi.org/10.1007/s13353-021-00609-4 |
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author | Tyczewska, Agata Gracz-Bernaciak, Joanna Szymkowiak, Jakub Twardowski, Tomasz |
author_facet | Tyczewska, Agata Gracz-Bernaciak, Joanna Szymkowiak, Jakub Twardowski, Tomasz |
author_sort | Tyczewska, Agata |
collection | PubMed |
description | DNA methylation plays a crucial role in the regulation of gene expression, activity of transposable elements, defense against foreign DNA, and inheritance of specific gene expression patterns. The link between stress exposure and sequence-specific changes in DNA methylation was hypothetical until it was shown that stresses can induce changes in the gene expression through hypomethylation or hypermethylation of DNA. To detect changes in DNA methylation under herbicide stress in two local Zea mays inbred lines exhibiting differential susceptibility to Roundup®, the methylation-sensitive amplified polymorphism (MSAP) technique was used. The overall DNA methylation levels were determined at approximately 60% for both tested lines. The most significant changes were observed for the more sensitive Z. mays line, where 6 h after the herbicide application, a large increase in the level of DNA methylation (attributed to the increase in fully methylated bands (18.65%)) was noted. DNA sequencing revealed that changes in DNA methylation profiles occurred in genes encoding heat shock proteins, membrane proteins, transporters, kinases, lipases, methyltransferases, zinc-finger proteins, cytochromes, and transposons. Herbicide stress-induced changes depended on the Z. mays variety, and the large increase in DNA methylation level in the sensitive line resulted in a lower ability to cope with stress conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13353-021-00609-4. |
format | Online Article Text |
id | pubmed-8032638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-80326382021-04-27 Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance Tyczewska, Agata Gracz-Bernaciak, Joanna Szymkowiak, Jakub Twardowski, Tomasz J Appl Genet Plant Genetics • Original Paper DNA methylation plays a crucial role in the regulation of gene expression, activity of transposable elements, defense against foreign DNA, and inheritance of specific gene expression patterns. The link between stress exposure and sequence-specific changes in DNA methylation was hypothetical until it was shown that stresses can induce changes in the gene expression through hypomethylation or hypermethylation of DNA. To detect changes in DNA methylation under herbicide stress in two local Zea mays inbred lines exhibiting differential susceptibility to Roundup®, the methylation-sensitive amplified polymorphism (MSAP) technique was used. The overall DNA methylation levels were determined at approximately 60% for both tested lines. The most significant changes were observed for the more sensitive Z. mays line, where 6 h after the herbicide application, a large increase in the level of DNA methylation (attributed to the increase in fully methylated bands (18.65%)) was noted. DNA sequencing revealed that changes in DNA methylation profiles occurred in genes encoding heat shock proteins, membrane proteins, transporters, kinases, lipases, methyltransferases, zinc-finger proteins, cytochromes, and transposons. Herbicide stress-induced changes depended on the Z. mays variety, and the large increase in DNA methylation level in the sensitive line resulted in a lower ability to cope with stress conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13353-021-00609-4. Springer Berlin Heidelberg 2021-01-29 2021 /pmc/articles/PMC8032638/ /pubmed/33512663 http://dx.doi.org/10.1007/s13353-021-00609-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Plant Genetics • Original Paper Tyczewska, Agata Gracz-Bernaciak, Joanna Szymkowiak, Jakub Twardowski, Tomasz Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance |
title | Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance |
title_full | Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance |
title_fullStr | Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance |
title_full_unstemmed | Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance |
title_short | Herbicide stress-induced DNA methylation changes in two Zea mays inbred lines differing in Roundup® resistance |
title_sort | herbicide stress-induced dna methylation changes in two zea mays inbred lines differing in roundup® resistance |
topic | Plant Genetics • Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032638/ https://www.ncbi.nlm.nih.gov/pubmed/33512663 http://dx.doi.org/10.1007/s13353-021-00609-4 |
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