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Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy

Fluorescence anisotropy (FA) is a powerful technique for the discovery of protein inhibitors in a high-throughput manner. In this study, we sought to develop new universal FA-based assays for the evaluation of compounds targeting mRNA 5′ cap-binding proteins of therapeutic interest, including eukary...

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Detalles Bibliográficos
Autores principales:	Wojtczak, Anna, Kasprzyk, Renata, Warmiński, Marcin, Ubych, Krystian, Kubacka, Dorota, Sikorski, Pawel J., Jemielity, Jacek, Kowalska, Joanna
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032668/ https://www.ncbi.nlm.nih.gov/pubmed/33833335 http://dx.doi.org/10.1038/s41598-021-87306-8

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