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Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium

The present study involved in vivo evaluation of the growth promoting effects of thymol and thymol nanoemulsion and their protection against Salmonella Typhimurium infection in broilers. One-day old 2400 chicks were randomly divided into eight groups; negative and positive control groups fed basal d...

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Autores principales: Ibrahim, Doaa, Abdelfattah-Hassan, Ahmed, Badawi, M., Ismail, Tamer Ahmed, Bendary, Mahmoud M., Abdelaziz, Adel M., Mosbah, Rasha A., Mohamed, Dalia Ibrahim, Arisha, Ahmed H., El-Hamid, Marwa I. Abd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032708/
https://www.ncbi.nlm.nih.gov/pubmed/33833292
http://dx.doi.org/10.1038/s41598-021-86990-w
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author Ibrahim, Doaa
Abdelfattah-Hassan, Ahmed
Badawi, M.
Ismail, Tamer Ahmed
Bendary, Mahmoud M.
Abdelaziz, Adel M.
Mosbah, Rasha A.
Mohamed, Dalia Ibrahim
Arisha, Ahmed H.
El-Hamid, Marwa I. Abd
author_facet Ibrahim, Doaa
Abdelfattah-Hassan, Ahmed
Badawi, M.
Ismail, Tamer Ahmed
Bendary, Mahmoud M.
Abdelaziz, Adel M.
Mosbah, Rasha A.
Mohamed, Dalia Ibrahim
Arisha, Ahmed H.
El-Hamid, Marwa I. Abd
author_sort Ibrahim, Doaa
collection PubMed
description The present study involved in vivo evaluation of the growth promoting effects of thymol and thymol nanoemulsion and their protection against Salmonella Typhimurium infection in broilers. One-day old 2400 chicks were randomly divided into eight groups; negative and positive control groups fed basal diet without additives and thymol and thymol nanoemulsion groups (0.25, 0.5 and 1% each). At d 23, all chicks except negative control were challenged with S. Typhimurium. Over the total growing period, birds fed 1% thymol nanoemulsion showed better growth performance even after S. Typhimurium challenge, which came parallel with upregulation of digestive enzyme genes (AMY2A, PNLIP and CCK). Additionally, higher levels of thymol nanoemulsion upregulated the expression of MUC-2, FABP2, IL-10, IgA and tight junction proteins genes and downregulated IL-2 and IL-6 genes expression. Moreover, 1% thymol nanoemulsion, and to lesser extent 0.5% thymol nanoemulsion and 1% thymol, corrected the histological alterations of cecum and liver postinfection. Finally, supplementation of 1% thymol, 0.5 and 1% thymol nanoemulsion led to increased Lactobacilli counts and decreased S. Typhimurium populations and downregulated invA gene expression postinfection. This first report of supplying thymol nanoemulsion in broiler diets proved that 1% nano-thymol is a potential growth promoting and antibacterial agent.
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spelling pubmed-80327082021-04-09 Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium Ibrahim, Doaa Abdelfattah-Hassan, Ahmed Badawi, M. Ismail, Tamer Ahmed Bendary, Mahmoud M. Abdelaziz, Adel M. Mosbah, Rasha A. Mohamed, Dalia Ibrahim Arisha, Ahmed H. El-Hamid, Marwa I. Abd Sci Rep Article The present study involved in vivo evaluation of the growth promoting effects of thymol and thymol nanoemulsion and their protection against Salmonella Typhimurium infection in broilers. One-day old 2400 chicks were randomly divided into eight groups; negative and positive control groups fed basal diet without additives and thymol and thymol nanoemulsion groups (0.25, 0.5 and 1% each). At d 23, all chicks except negative control were challenged with S. Typhimurium. Over the total growing period, birds fed 1% thymol nanoemulsion showed better growth performance even after S. Typhimurium challenge, which came parallel with upregulation of digestive enzyme genes (AMY2A, PNLIP and CCK). Additionally, higher levels of thymol nanoemulsion upregulated the expression of MUC-2, FABP2, IL-10, IgA and tight junction proteins genes and downregulated IL-2 and IL-6 genes expression. Moreover, 1% thymol nanoemulsion, and to lesser extent 0.5% thymol nanoemulsion and 1% thymol, corrected the histological alterations of cecum and liver postinfection. Finally, supplementation of 1% thymol, 0.5 and 1% thymol nanoemulsion led to increased Lactobacilli counts and decreased S. Typhimurium populations and downregulated invA gene expression postinfection. This first report of supplying thymol nanoemulsion in broiler diets proved that 1% nano-thymol is a potential growth promoting and antibacterial agent. Nature Publishing Group UK 2021-04-08 /pmc/articles/PMC8032708/ /pubmed/33833292 http://dx.doi.org/10.1038/s41598-021-86990-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ibrahim, Doaa
Abdelfattah-Hassan, Ahmed
Badawi, M.
Ismail, Tamer Ahmed
Bendary, Mahmoud M.
Abdelaziz, Adel M.
Mosbah, Rasha A.
Mohamed, Dalia Ibrahim
Arisha, Ahmed H.
El-Hamid, Marwa I. Abd
Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
title Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
title_full Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
title_fullStr Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
title_full_unstemmed Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
title_short Thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against Salmonella Typhimurium
title_sort thymol nanoemulsion promoted broiler chicken’s growth, gastrointestinal barrier and bacterial community and conferred protection against salmonella typhimurium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032708/
https://www.ncbi.nlm.nih.gov/pubmed/33833292
http://dx.doi.org/10.1038/s41598-021-86990-w
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