Cargando…

Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf

Sepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistan...

Descripción completa

Detalles Bibliográficos
Autores principales: Pas, Mathilde L., Vanneste, Kevin, Bokma, Jade, Van Driessche, Laura, De Keersmaecker, Sigrid C. J., Roosens, Nancy H., Haesebrouck, Freddy, Boyen, Filip, Pardon, Bart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032891/
https://www.ncbi.nlm.nih.gov/pubmed/33842574
http://dx.doi.org/10.3389/fvets.2021.631716
_version_ 1783676303543631872
author Pas, Mathilde L.
Vanneste, Kevin
Bokma, Jade
Van Driessche, Laura
De Keersmaecker, Sigrid C. J.
Roosens, Nancy H.
Haesebrouck, Freddy
Boyen, Filip
Pardon, Bart
author_facet Pas, Mathilde L.
Vanneste, Kevin
Bokma, Jade
Van Driessche, Laura
De Keersmaecker, Sigrid C. J.
Roosens, Nancy H.
Haesebrouck, Freddy
Boyen, Filip
Pardon, Bart
author_sort Pas, Mathilde L.
collection PubMed
description Sepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistant Raoultella ornithinolytica, an emerging pathogen in human medicine, in a calf with suspected sepsis. R. ornithinolytica was identified by MALDI-TOF MS from blood cultures of a critically ill calf. Susceptibility testing showed phenotypic resistance against ampicillin, gentamicin, potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as R. ornithinolytica and the multidrug resistant character of the isolate. Antimicrobial resistance genes acting against aminoglycosides, beta-lactam antibiotics, fosfomycin, quinolones, sulphonamides, trimethoprim and tetracyclines were found. The calf recovered after empirical parenteral therapy with enrofloxacin and sodium penicillin for seven days. Ancillary therapy consisted of fluid therapy, ketoprofen and doxapram hydrochloride. To the authors' knowledge, this is the first report characterizing a multidrug resistant R. ornithinolytica isolate from blood culture in cattle. It is currently unknown whether animals and farms may act as reservoirs for multidrug resistant R. ornithinolytica strains.
format Online
Article
Text
id pubmed-8032891
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80328912021-04-10 Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf Pas, Mathilde L. Vanneste, Kevin Bokma, Jade Van Driessche, Laura De Keersmaecker, Sigrid C. J. Roosens, Nancy H. Haesebrouck, Freddy Boyen, Filip Pardon, Bart Front Vet Sci Veterinary Science Sepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistant Raoultella ornithinolytica, an emerging pathogen in human medicine, in a calf with suspected sepsis. R. ornithinolytica was identified by MALDI-TOF MS from blood cultures of a critically ill calf. Susceptibility testing showed phenotypic resistance against ampicillin, gentamicin, potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as R. ornithinolytica and the multidrug resistant character of the isolate. Antimicrobial resistance genes acting against aminoglycosides, beta-lactam antibiotics, fosfomycin, quinolones, sulphonamides, trimethoprim and tetracyclines were found. The calf recovered after empirical parenteral therapy with enrofloxacin and sodium penicillin for seven days. Ancillary therapy consisted of fluid therapy, ketoprofen and doxapram hydrochloride. To the authors' knowledge, this is the first report characterizing a multidrug resistant R. ornithinolytica isolate from blood culture in cattle. It is currently unknown whether animals and farms may act as reservoirs for multidrug resistant R. ornithinolytica strains. Frontiers Media S.A. 2021-03-26 /pmc/articles/PMC8032891/ /pubmed/33842574 http://dx.doi.org/10.3389/fvets.2021.631716 Text en Copyright © 2021 Pas, Vanneste, Bokma, Van Driessche, De Keersmaecker, Roosens, Haesebrouck, Boyen and Pardon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Pas, Mathilde L.
Vanneste, Kevin
Bokma, Jade
Van Driessche, Laura
De Keersmaecker, Sigrid C. J.
Roosens, Nancy H.
Haesebrouck, Freddy
Boyen, Filip
Pardon, Bart
Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
title Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
title_full Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
title_fullStr Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
title_full_unstemmed Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
title_short Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
title_sort case report: multidrug resistant raoultella ornithinolytica in a septicemic calf
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032891/
https://www.ncbi.nlm.nih.gov/pubmed/33842574
http://dx.doi.org/10.3389/fvets.2021.631716
work_keys_str_mv AT pasmathildel casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT vannestekevin casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT bokmajade casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT vandriesschelaura casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT dekeersmaeckersigridcj casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT roosensnancyh casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT haesebrouckfreddy casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT boyenfilip casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf
AT pardonbart casereportmultidrugresistantraoultellaornithinolyticainasepticemiccalf