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Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8

In this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 10(5) Da. The structural characterization of EPS indicated that the EP...

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Autores principales: Wu, Junrui, Yan, Danli, Liu, Yumeng, Luo, Xue, Li, Yang, Cao, Chengxu, Li, Mo, Han, Qi, Wang, Cong, Wu, Rina, Zhang, Lanwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033024/
https://www.ncbi.nlm.nih.gov/pubmed/33841368
http://dx.doi.org/10.3389/fmicb.2021.644226
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author Wu, Junrui
Yan, Danli
Liu, Yumeng
Luo, Xue
Li, Yang
Cao, Chengxu
Li, Mo
Han, Qi
Wang, Cong
Wu, Rina
Zhang, Lanwei
author_facet Wu, Junrui
Yan, Danli
Liu, Yumeng
Luo, Xue
Li, Yang
Cao, Chengxu
Li, Mo
Han, Qi
Wang, Cong
Wu, Rina
Zhang, Lanwei
author_sort Wu, Junrui
collection PubMed
description In this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 10(5) Da. The structural characterization of EPS indicated that the EPS belonged to the class polysaccharide, mainly composed of glucan and also contained certain mannose residues that were found to be connected by α-1,6 glycosidic bonds. Moreover, the results demonstrated that EPS displayed a significant capacity to scavenge free radical to some extent, and this anti-oxidant potential was found to be concentration dependent. The results further revealed that EPS displayed a significant inhibitory potential on the growth of HepG2 cells by promoting apoptosis and induced cell cycle arrest in G1 and G2 phases. Overall, these results suggested that EPS can be explored as a possible anti-cancer agent.
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spelling pubmed-80330242021-04-10 Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 Wu, Junrui Yan, Danli Liu, Yumeng Luo, Xue Li, Yang Cao, Chengxu Li, Mo Han, Qi Wang, Cong Wu, Rina Zhang, Lanwei Front Microbiol Microbiology In this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 10(5) Da. The structural characterization of EPS indicated that the EPS belonged to the class polysaccharide, mainly composed of glucan and also contained certain mannose residues that were found to be connected by α-1,6 glycosidic bonds. Moreover, the results demonstrated that EPS displayed a significant capacity to scavenge free radical to some extent, and this anti-oxidant potential was found to be concentration dependent. The results further revealed that EPS displayed a significant inhibitory potential on the growth of HepG2 cells by promoting apoptosis and induced cell cycle arrest in G1 and G2 phases. Overall, these results suggested that EPS can be explored as a possible anti-cancer agent. Frontiers Media S.A. 2021-03-26 /pmc/articles/PMC8033024/ /pubmed/33841368 http://dx.doi.org/10.3389/fmicb.2021.644226 Text en Copyright © 2021 Wu, Yan, Liu, Luo, Li, Cao, Li, Han, Wang, Wu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wu, Junrui
Yan, Danli
Liu, Yumeng
Luo, Xue
Li, Yang
Cao, Chengxu
Li, Mo
Han, Qi
Wang, Cong
Wu, Rina
Zhang, Lanwei
Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
title Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
title_full Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
title_fullStr Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
title_full_unstemmed Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
title_short Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
title_sort purification, structural characteristics, and biological activities of exopolysaccharide isolated from leuconostoc mesenteroides sn-8
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033024/
https://www.ncbi.nlm.nih.gov/pubmed/33841368
http://dx.doi.org/10.3389/fmicb.2021.644226
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