Cargando…
lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene
Fat deposition is one of the most important traits that are mediated by a set of complex regulatory factors in meat animals. Several researches have revealed the significant role of long non-coding RNAs (lncRNAs) in fat deposition while the precise regulatory mechanism is still largely elusive. In t...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033173/ https://www.ncbi.nlm.nih.gov/pubmed/33841496 http://dx.doi.org/10.3389/fgene.2021.626158 |
_version_ | 1783676357558927360 |
---|---|
author | Zhu, Ruirui Feng, Xue Wei, Yutong Guo, Duo Li, Jiaojiao Liu, Qingyou Jiang, Jianrong Shi, Deshun Huang, Jieping |
author_facet | Zhu, Ruirui Feng, Xue Wei, Yutong Guo, Duo Li, Jiaojiao Liu, Qingyou Jiang, Jianrong Shi, Deshun Huang, Jieping |
author_sort | Zhu, Ruirui |
collection | PubMed |
description | Fat deposition is one of the most important traits that are mediated by a set of complex regulatory factors in meat animals. Several researches have revealed the significant role of long non-coding RNAs (lncRNAs) in fat deposition while the precise regulatory mechanism is still largely elusive. In this study, we investigated the lncRNA profiles of adipose and muscle tissues in buffalo by using the Illumina HiSeq 3000 platform. In total, 43,809 lncRNAs were finally identified based on the computer algorithm. A comparison analysis revealed 241 lncRNAs that are differentially expressed (DE) in adipose and muscle tissues. We focused on lncSAMM50, a DE lncRNA that has a high expression in adipose tissue. Sequence alignment showed that lncSAMM50 is transcribed from the antisense strand of the upstream region of sorting and assembly machinery component 50 homolog (SAMM50), a gene involved in the function of mitochondrion and is subsequently demonstrated to inhibit the adipogenic differentiation of 3T3-L1 adipocyte cells in this study. lncSAMM50 is highly expressed in adipose tissue and upregulated in the mature adipocytes and mainly exists in the nucleus. Gain-of-function experiments demonstrated that lncSAMM50 promotes the adipogenic differentiation by upregulating adipogenic markers but with no effect on its host gene SAMM50 in buffalo adipocytes. These results indicate that lncSAMM50 enhances fat deposition in buffalo and provide a new factor for the regulatory network of adipogenesis. |
format | Online Article Text |
id | pubmed-8033173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80331732021-04-10 lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene Zhu, Ruirui Feng, Xue Wei, Yutong Guo, Duo Li, Jiaojiao Liu, Qingyou Jiang, Jianrong Shi, Deshun Huang, Jieping Front Genet Genetics Fat deposition is one of the most important traits that are mediated by a set of complex regulatory factors in meat animals. Several researches have revealed the significant role of long non-coding RNAs (lncRNAs) in fat deposition while the precise regulatory mechanism is still largely elusive. In this study, we investigated the lncRNA profiles of adipose and muscle tissues in buffalo by using the Illumina HiSeq 3000 platform. In total, 43,809 lncRNAs were finally identified based on the computer algorithm. A comparison analysis revealed 241 lncRNAs that are differentially expressed (DE) in adipose and muscle tissues. We focused on lncSAMM50, a DE lncRNA that has a high expression in adipose tissue. Sequence alignment showed that lncSAMM50 is transcribed from the antisense strand of the upstream region of sorting and assembly machinery component 50 homolog (SAMM50), a gene involved in the function of mitochondrion and is subsequently demonstrated to inhibit the adipogenic differentiation of 3T3-L1 adipocyte cells in this study. lncSAMM50 is highly expressed in adipose tissue and upregulated in the mature adipocytes and mainly exists in the nucleus. Gain-of-function experiments demonstrated that lncSAMM50 promotes the adipogenic differentiation by upregulating adipogenic markers but with no effect on its host gene SAMM50 in buffalo adipocytes. These results indicate that lncSAMM50 enhances fat deposition in buffalo and provide a new factor for the regulatory network of adipogenesis. Frontiers Media S.A. 2021-03-26 /pmc/articles/PMC8033173/ /pubmed/33841496 http://dx.doi.org/10.3389/fgene.2021.626158 Text en Copyright © 2021 Zhu, Feng, Wei, Guo, Li, Liu, Jiang, Shi and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zhu, Ruirui Feng, Xue Wei, Yutong Guo, Duo Li, Jiaojiao Liu, Qingyou Jiang, Jianrong Shi, Deshun Huang, Jieping lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene |
title | lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene |
title_full | lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene |
title_fullStr | lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene |
title_full_unstemmed | lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene |
title_short | lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene |
title_sort | lncsamm50 enhances adipogenic differentiation of buffalo adipocytes with no effect on its host gene |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033173/ https://www.ncbi.nlm.nih.gov/pubmed/33841496 http://dx.doi.org/10.3389/fgene.2021.626158 |
work_keys_str_mv | AT zhuruirui lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT fengxue lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT weiyutong lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT guoduo lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT lijiaojiao lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT liuqingyou lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT jiangjianrong lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT shideshun lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene AT huangjieping lncsamm50enhancesadipogenicdifferentiationofbuffaloadipocyteswithnoeffectonitshostgene |