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Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
INTRODUCTION: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinar...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033244/ https://www.ncbi.nlm.nih.gov/pubmed/33850357 http://dx.doi.org/10.4103/iju.IJU_330_20 |
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author | Cheriyan, Abhilash Nellickal, Arun Jose John, Nirmal Thampi Jeyaseelan, Lakshmanan Kumar, Santosh Devasia, Antony Kekre, Nitin |
author_facet | Cheriyan, Abhilash Nellickal, Arun Jose John, Nirmal Thampi Jeyaseelan, Lakshmanan Kumar, Santosh Devasia, Antony Kekre, Nitin |
author_sort | Cheriyan, Abhilash |
collection | PubMed |
description | INTRODUCTION: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinary aquaporin-1 (uAQP-1), an upcoming urinary biomarker, for renal cell carcinoma. MATERIALS AND METHODS: In this prospective biomarker study, urine samples were obtained preoperatively from 36 patients with an imaged renal mass suggestive of RCC and 24 healthy age-matched controls, chosen from among voluntary kidney donors. uAQP-1 concentrations were estimated with a sensitive and specific enzyme-linked immunosorbent assay (ELISA) and normalized by estimation of urinary creatinine. The Mann–Whitney U-test was used to compare differences between any two groups. A receiver operator characteristic (ROC) curve was plotted to analyze the diagnostic accuracy of uAQP-1 for RCC. RESULTS: The median uAQP-1 concentration among the cases and controls was 8.78 ng/mg creatinine (interquartile range [IQR]: 5.56–12.67) and 9.52 ng/mg creatinine (IQR: 5.55–12.45), respectively. There was no significant difference in uAQP-1 concentrations between the two groups. ROC analysis showed that, for a cutoff value of 8 ng/mg creatinine, the sensitivity and specificity of uAQP-1 as a diagnostic test were 47.2% and 66.7%, respectively, and area under the curve was 0.52 (95% confidence interval: 0.42–0.62). CONCLUSIONS: uAQP-1 concentrations did not discriminate between healthy individuals and patients with RCC. The results of this study suggest that uAQP-1 may not be a suitable diagnostic biomarker for RCC in the study population. |
format | Online Article Text |
id | pubmed-8033244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-80332442021-04-12 Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma Cheriyan, Abhilash Nellickal, Arun Jose John, Nirmal Thampi Jeyaseelan, Lakshmanan Kumar, Santosh Devasia, Antony Kekre, Nitin Indian J Urol Original Article INTRODUCTION: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinary aquaporin-1 (uAQP-1), an upcoming urinary biomarker, for renal cell carcinoma. MATERIALS AND METHODS: In this prospective biomarker study, urine samples were obtained preoperatively from 36 patients with an imaged renal mass suggestive of RCC and 24 healthy age-matched controls, chosen from among voluntary kidney donors. uAQP-1 concentrations were estimated with a sensitive and specific enzyme-linked immunosorbent assay (ELISA) and normalized by estimation of urinary creatinine. The Mann–Whitney U-test was used to compare differences between any two groups. A receiver operator characteristic (ROC) curve was plotted to analyze the diagnostic accuracy of uAQP-1 for RCC. RESULTS: The median uAQP-1 concentration among the cases and controls was 8.78 ng/mg creatinine (interquartile range [IQR]: 5.56–12.67) and 9.52 ng/mg creatinine (IQR: 5.55–12.45), respectively. There was no significant difference in uAQP-1 concentrations between the two groups. ROC analysis showed that, for a cutoff value of 8 ng/mg creatinine, the sensitivity and specificity of uAQP-1 as a diagnostic test were 47.2% and 66.7%, respectively, and area under the curve was 0.52 (95% confidence interval: 0.42–0.62). CONCLUSIONS: uAQP-1 concentrations did not discriminate between healthy individuals and patients with RCC. The results of this study suggest that uAQP-1 may not be a suitable diagnostic biomarker for RCC in the study population. Wolters Kluwer - Medknow 2021 2021-01-01 /pmc/articles/PMC8033244/ /pubmed/33850357 http://dx.doi.org/10.4103/iju.IJU_330_20 Text en Copyright: © 2021 Indian Journal of Urology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Cheriyan, Abhilash Nellickal, Arun Jose John, Nirmal Thampi Jeyaseelan, Lakshmanan Kumar, Santosh Devasia, Antony Kekre, Nitin Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title | Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_full | Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_fullStr | Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_full_unstemmed | Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_short | Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_sort | diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033244/ https://www.ncbi.nlm.nih.gov/pubmed/33850357 http://dx.doi.org/10.4103/iju.IJU_330_20 |
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