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Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma

INTRODUCTION: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinar...

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Autores principales: Cheriyan, Abhilash, Nellickal, Arun Jose, John, Nirmal Thampi, Jeyaseelan, Lakshmanan, Kumar, Santosh, Devasia, Antony, Kekre, Nitin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033244/
https://www.ncbi.nlm.nih.gov/pubmed/33850357
http://dx.doi.org/10.4103/iju.IJU_330_20
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author Cheriyan, Abhilash
Nellickal, Arun Jose
John, Nirmal Thampi
Jeyaseelan, Lakshmanan
Kumar, Santosh
Devasia, Antony
Kekre, Nitin
author_facet Cheriyan, Abhilash
Nellickal, Arun Jose
John, Nirmal Thampi
Jeyaseelan, Lakshmanan
Kumar, Santosh
Devasia, Antony
Kekre, Nitin
author_sort Cheriyan, Abhilash
collection PubMed
description INTRODUCTION: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinary aquaporin-1 (uAQP-1), an upcoming urinary biomarker, for renal cell carcinoma. MATERIALS AND METHODS: In this prospective biomarker study, urine samples were obtained preoperatively from 36 patients with an imaged renal mass suggestive of RCC and 24 healthy age-matched controls, chosen from among voluntary kidney donors. uAQP-1 concentrations were estimated with a sensitive and specific enzyme-linked immunosorbent assay (ELISA) and normalized by estimation of urinary creatinine. The Mann–Whitney U-test was used to compare differences between any two groups. A receiver operator characteristic (ROC) curve was plotted to analyze the diagnostic accuracy of uAQP-1 for RCC. RESULTS: The median uAQP-1 concentration among the cases and controls was 8.78 ng/mg creatinine (interquartile range [IQR]: 5.56–12.67) and 9.52 ng/mg creatinine (IQR: 5.55–12.45), respectively. There was no significant difference in uAQP-1 concentrations between the two groups. ROC analysis showed that, for a cutoff value of 8 ng/mg creatinine, the sensitivity and specificity of uAQP-1 as a diagnostic test were 47.2% and 66.7%, respectively, and area under the curve was 0.52 (95% confidence interval: 0.42–0.62). CONCLUSIONS: uAQP-1 concentrations did not discriminate between healthy individuals and patients with RCC. The results of this study suggest that uAQP-1 may not be a suitable diagnostic biomarker for RCC in the study population.
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spelling pubmed-80332442021-04-12 Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma Cheriyan, Abhilash Nellickal, Arun Jose John, Nirmal Thampi Jeyaseelan, Lakshmanan Kumar, Santosh Devasia, Antony Kekre, Nitin Indian J Urol Original Article INTRODUCTION: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinary aquaporin-1 (uAQP-1), an upcoming urinary biomarker, for renal cell carcinoma. MATERIALS AND METHODS: In this prospective biomarker study, urine samples were obtained preoperatively from 36 patients with an imaged renal mass suggestive of RCC and 24 healthy age-matched controls, chosen from among voluntary kidney donors. uAQP-1 concentrations were estimated with a sensitive and specific enzyme-linked immunosorbent assay (ELISA) and normalized by estimation of urinary creatinine. The Mann–Whitney U-test was used to compare differences between any two groups. A receiver operator characteristic (ROC) curve was plotted to analyze the diagnostic accuracy of uAQP-1 for RCC. RESULTS: The median uAQP-1 concentration among the cases and controls was 8.78 ng/mg creatinine (interquartile range [IQR]: 5.56–12.67) and 9.52 ng/mg creatinine (IQR: 5.55–12.45), respectively. There was no significant difference in uAQP-1 concentrations between the two groups. ROC analysis showed that, for a cutoff value of 8 ng/mg creatinine, the sensitivity and specificity of uAQP-1 as a diagnostic test were 47.2% and 66.7%, respectively, and area under the curve was 0.52 (95% confidence interval: 0.42–0.62). CONCLUSIONS: uAQP-1 concentrations did not discriminate between healthy individuals and patients with RCC. The results of this study suggest that uAQP-1 may not be a suitable diagnostic biomarker for RCC in the study population. Wolters Kluwer - Medknow 2021 2021-01-01 /pmc/articles/PMC8033244/ /pubmed/33850357 http://dx.doi.org/10.4103/iju.IJU_330_20 Text en Copyright: © 2021 Indian Journal of Urology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Cheriyan, Abhilash
Nellickal, Arun Jose
John, Nirmal Thampi
Jeyaseelan, Lakshmanan
Kumar, Santosh
Devasia, Antony
Kekre, Nitin
Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
title Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
title_full Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
title_fullStr Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
title_full_unstemmed Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
title_short Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
title_sort diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033244/
https://www.ncbi.nlm.nih.gov/pubmed/33850357
http://dx.doi.org/10.4103/iju.IJU_330_20
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