Cargando…

Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice

BACKGROUND: Morus alba L. bark has been widely used in traditional medicine for treating several inflammatory diseases, such as hypertension, diabetes mellitus and coughing; however, the molecular mechanisms underlying its anti-inflammatory effects are not well understood. METHODS: We examined the e...

Descripción completa

Detalles Bibliográficos
Autores principales: Umeyama, Lin, Hardianti, Besse, Kasahara, Shiori, Dibwe, Dya Fita, Awale, Suresh, Yokoyama, Satoru, Hayakawa, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033707/
https://www.ncbi.nlm.nih.gov/pubmed/33836731
http://dx.doi.org/10.1186/s12906-021-03291-5
_version_ 1783676451059400704
author Umeyama, Lin
Hardianti, Besse
Kasahara, Shiori
Dibwe, Dya Fita
Awale, Suresh
Yokoyama, Satoru
Hayakawa, Yoshihiro
author_facet Umeyama, Lin
Hardianti, Besse
Kasahara, Shiori
Dibwe, Dya Fita
Awale, Suresh
Yokoyama, Satoru
Hayakawa, Yoshihiro
author_sort Umeyama, Lin
collection PubMed
description BACKGROUND: Morus alba L. bark has been widely used in traditional medicine for treating several inflammatory diseases, such as hypertension, diabetes mellitus and coughing; however, the molecular mechanisms underlying its anti-inflammatory effects are not well understood. METHODS: We examined the effects of an extract of Morus alba L. bark (MabE) on Toll-like receptor (TLR) ligand-induced activation of RAW264.7 macrophages using a luciferase reporter assay and immunoassays. For the in vivo experiment, we used an imiquimod-induced ear edema model to examine the anti-inflammatory effects of MabE. RESULTS: MabE inhibited the TLR ligand-induced activation of NF-κB in RAW264.7 cells without affecting their viability. Consistent with the inhibition of NF-κB activation, MabE also inhibited the production of IL-6 and IL-1β from TLR ligand-treated RAW264.7 cells. In vivo MabE treatment inhibited the ear swelling of IMQ-treated mice, in addition to the mRNA expression of IL-17A, IL-1β and COX-2. The increases in splenic γδT cells in IMQ-treated mice and the production of IL-17A from splenocytes were significantly inhibited by MabE treatment. CONCLUSION: Our study suggests that the anti-inflammatory effects of MabE on the activation of the macrophage cell line RAW246.7 by TLRs and IMQ-induced ear edema are through the inhibition of NF-κB activation and IL-17A-producing γδT cells, respectively. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-021-03291-5.
format Online
Article
Text
id pubmed-8033707
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-80337072021-04-09 Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice Umeyama, Lin Hardianti, Besse Kasahara, Shiori Dibwe, Dya Fita Awale, Suresh Yokoyama, Satoru Hayakawa, Yoshihiro BMC Complement Med Ther Research Article BACKGROUND: Morus alba L. bark has been widely used in traditional medicine for treating several inflammatory diseases, such as hypertension, diabetes mellitus and coughing; however, the molecular mechanisms underlying its anti-inflammatory effects are not well understood. METHODS: We examined the effects of an extract of Morus alba L. bark (MabE) on Toll-like receptor (TLR) ligand-induced activation of RAW264.7 macrophages using a luciferase reporter assay and immunoassays. For the in vivo experiment, we used an imiquimod-induced ear edema model to examine the anti-inflammatory effects of MabE. RESULTS: MabE inhibited the TLR ligand-induced activation of NF-κB in RAW264.7 cells without affecting their viability. Consistent with the inhibition of NF-κB activation, MabE also inhibited the production of IL-6 and IL-1β from TLR ligand-treated RAW264.7 cells. In vivo MabE treatment inhibited the ear swelling of IMQ-treated mice, in addition to the mRNA expression of IL-17A, IL-1β and COX-2. The increases in splenic γδT cells in IMQ-treated mice and the production of IL-17A from splenocytes were significantly inhibited by MabE treatment. CONCLUSION: Our study suggests that the anti-inflammatory effects of MabE on the activation of the macrophage cell line RAW246.7 by TLRs and IMQ-induced ear edema are through the inhibition of NF-κB activation and IL-17A-producing γδT cells, respectively. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-021-03291-5. BioMed Central 2021-04-09 /pmc/articles/PMC8033707/ /pubmed/33836731 http://dx.doi.org/10.1186/s12906-021-03291-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Umeyama, Lin
Hardianti, Besse
Kasahara, Shiori
Dibwe, Dya Fita
Awale, Suresh
Yokoyama, Satoru
Hayakawa, Yoshihiro
Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
title Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
title_full Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
title_fullStr Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
title_full_unstemmed Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
title_short Anti-inflammatory effects of Morus alba Linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
title_sort anti-inflammatory effects of morus alba linne bark on the activation of toll-like receptors and imiquimod-induced ear edema in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033707/
https://www.ncbi.nlm.nih.gov/pubmed/33836731
http://dx.doi.org/10.1186/s12906-021-03291-5
work_keys_str_mv AT umeyamalin antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice
AT hardiantibesse antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice
AT kasaharashiori antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice
AT dibwedyafita antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice
AT awalesuresh antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice
AT yokoyamasatoru antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice
AT hayakawayoshihiro antiinflammatoryeffectsofmorusalbalinnebarkontheactivationoftolllikereceptorsandimiquimodinducedearedemainmice