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Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis
BACKGROUND: Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa ti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033732/ https://www.ncbi.nlm.nih.gov/pubmed/33836777 http://dx.doi.org/10.1186/s12920-021-00949-4 |
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author | Wei, Xian Xu, Man Wang, Chao Fang, Shengjian Zhang, Yu Wang, Weihua |
author_facet | Wei, Xian Xu, Man Wang, Chao Fang, Shengjian Zhang, Yu Wang, Weihua |
author_sort | Wei, Xian |
collection | PubMed |
description | BACKGROUND: Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa tissue and mRNA regulatory relationship was examined among patients with or without AR. METHODS: Microarray assays were performed and the differential expressions of lncRNAs or mRNA were verified through RT-PCR. The lncRNA functions were annotated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The potential regulatory relationships between lncRNAs and the co-expressed mRNAs were analyzed using Cytoscape software. The expressions of specific lncRNAs and mRNAs were examined using an in vitro cell model. RESULTS: A total of 57 lncRNAs and 127 mRNAs were dysregulated in the nasal mucosa tissue of patients with AR, compared to those of patients without AR (fold change > 2.0 and P < 0.05). GO and pathway analysis indicated that the lncRNA–co-expressed mRNAs were enriched in several biological processes and cellular signaling pathways related to AR, such as positive regulation of the integrin biosynthetic process, cell adhesion, and leukocyte transendothelial migration. Some lncRNAs regulated the co-expressed genes in a cis- and/or trans-regulatory manner. Furthermore, allergen exposure significantly increased the expression of lnc-CXCL12-4, CXCL12, and CXCR4 in BEAS-2B cells compared to untreated cells (P < 0.01). CONCLUSION: The results of the present study suggest that lncRNAs participate in the biological pathways related to AR. Leukocyte transepithelial migration may be a potential target for lncRNAs to regulate allergic inflammation and CXCL12/CXCR4 axis plays an important role in the inflammatory process of AR. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12920-021-00949-4. |
format | Online Article Text |
id | pubmed-8033732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80337322021-04-09 Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis Wei, Xian Xu, Man Wang, Chao Fang, Shengjian Zhang, Yu Wang, Weihua BMC Med Genomics Research Article BACKGROUND: Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa tissue and mRNA regulatory relationship was examined among patients with or without AR. METHODS: Microarray assays were performed and the differential expressions of lncRNAs or mRNA were verified through RT-PCR. The lncRNA functions were annotated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The potential regulatory relationships between lncRNAs and the co-expressed mRNAs were analyzed using Cytoscape software. The expressions of specific lncRNAs and mRNAs were examined using an in vitro cell model. RESULTS: A total of 57 lncRNAs and 127 mRNAs were dysregulated in the nasal mucosa tissue of patients with AR, compared to those of patients without AR (fold change > 2.0 and P < 0.05). GO and pathway analysis indicated that the lncRNA–co-expressed mRNAs were enriched in several biological processes and cellular signaling pathways related to AR, such as positive regulation of the integrin biosynthetic process, cell adhesion, and leukocyte transendothelial migration. Some lncRNAs regulated the co-expressed genes in a cis- and/or trans-regulatory manner. Furthermore, allergen exposure significantly increased the expression of lnc-CXCL12-4, CXCL12, and CXCR4 in BEAS-2B cells compared to untreated cells (P < 0.01). CONCLUSION: The results of the present study suggest that lncRNAs participate in the biological pathways related to AR. Leukocyte transepithelial migration may be a potential target for lncRNAs to regulate allergic inflammation and CXCL12/CXCR4 axis plays an important role in the inflammatory process of AR. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12920-021-00949-4. BioMed Central 2021-04-09 /pmc/articles/PMC8033732/ /pubmed/33836777 http://dx.doi.org/10.1186/s12920-021-00949-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Wei, Xian Xu, Man Wang, Chao Fang, Shengjian Zhang, Yu Wang, Weihua Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis |
title | Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis |
title_full | Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis |
title_fullStr | Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis |
title_full_unstemmed | Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis |
title_short | Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis |
title_sort | genome-wide analysis of long noncoding rna expression profile in nasal mucosa with allergic rhinitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033732/ https://www.ncbi.nlm.nih.gov/pubmed/33836777 http://dx.doi.org/10.1186/s12920-021-00949-4 |
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