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The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization
BACKGROUND: ESCRT-III proteins are involved in many membrane remodeling processes including multivesicular body biogenesis as first discovered in yeast. In humans, ESCRT-III CHMP2 exists as two isoforms, CHMP2A and CHMP2B, but their physical characteristics have not been compared yet. RESULTS: Here,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033747/ https://www.ncbi.nlm.nih.gov/pubmed/33832485 http://dx.doi.org/10.1186/s12915-021-00983-9 |
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author | Alqabandi, Maryam de Franceschi, Nicola Maity, Sourav Miguet, Nolwenn Bally, Marta Roos, Wouter H. Weissenhorn, Winfried Bassereau, Patricia Mangenot, Stéphanie |
author_facet | Alqabandi, Maryam de Franceschi, Nicola Maity, Sourav Miguet, Nolwenn Bally, Marta Roos, Wouter H. Weissenhorn, Winfried Bassereau, Patricia Mangenot, Stéphanie |
author_sort | Alqabandi, Maryam |
collection | PubMed |
description | BACKGROUND: ESCRT-III proteins are involved in many membrane remodeling processes including multivesicular body biogenesis as first discovered in yeast. In humans, ESCRT-III CHMP2 exists as two isoforms, CHMP2A and CHMP2B, but their physical characteristics have not been compared yet. RESULTS: Here, we use a combination of techniques on biomimetic systems and purified proteins to study their affinity and effects on membranes. We establish that CHMP2B binding is enhanced in the presence of PI(4,5)P2 lipids. In contrast, CHMP2A does not display lipid specificity and requires CHMP3 for binding significantly to membranes. On the micrometer scale and at moderate bulk concentrations, CHMP2B forms a reticular structure on membranes whereas CHMP2A (+CHMP3) binds homogeneously. Thus, CHMP2A and CHMP2B unexpectedly induce different mechanical effects to membranes: CHMP2B strongly rigidifies them while CHMP2A (+CHMP3) has no significant effect. CONCLUSIONS: We therefore conclude that CHMP2B and CHMP2A exhibit different mechanical properties and might thus contribute differently to the diverse ESCRT-III-catalyzed membrane remodeling processes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-021-00983-9. |
format | Online Article Text |
id | pubmed-8033747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80337472021-04-09 The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization Alqabandi, Maryam de Franceschi, Nicola Maity, Sourav Miguet, Nolwenn Bally, Marta Roos, Wouter H. Weissenhorn, Winfried Bassereau, Patricia Mangenot, Stéphanie BMC Biol Research Article BACKGROUND: ESCRT-III proteins are involved in many membrane remodeling processes including multivesicular body biogenesis as first discovered in yeast. In humans, ESCRT-III CHMP2 exists as two isoforms, CHMP2A and CHMP2B, but their physical characteristics have not been compared yet. RESULTS: Here, we use a combination of techniques on biomimetic systems and purified proteins to study their affinity and effects on membranes. We establish that CHMP2B binding is enhanced in the presence of PI(4,5)P2 lipids. In contrast, CHMP2A does not display lipid specificity and requires CHMP3 for binding significantly to membranes. On the micrometer scale and at moderate bulk concentrations, CHMP2B forms a reticular structure on membranes whereas CHMP2A (+CHMP3) binds homogeneously. Thus, CHMP2A and CHMP2B unexpectedly induce different mechanical effects to membranes: CHMP2B strongly rigidifies them while CHMP2A (+CHMP3) has no significant effect. CONCLUSIONS: We therefore conclude that CHMP2B and CHMP2A exhibit different mechanical properties and might thus contribute differently to the diverse ESCRT-III-catalyzed membrane remodeling processes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-021-00983-9. BioMed Central 2021-04-08 /pmc/articles/PMC8033747/ /pubmed/33832485 http://dx.doi.org/10.1186/s12915-021-00983-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Alqabandi, Maryam de Franceschi, Nicola Maity, Sourav Miguet, Nolwenn Bally, Marta Roos, Wouter H. Weissenhorn, Winfried Bassereau, Patricia Mangenot, Stéphanie The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization |
title | The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization |
title_full | The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization |
title_fullStr | The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization |
title_full_unstemmed | The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization |
title_short | The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization |
title_sort | escrt-iii isoforms chmp2a and chmp2b display different effects on membranes upon polymerization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033747/ https://www.ncbi.nlm.nih.gov/pubmed/33832485 http://dx.doi.org/10.1186/s12915-021-00983-9 |
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