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Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress
Clematis is a superior perennial ornamental vine known for varied colors and shapes of its flowers. Clematis crassifolia is sensitive to high temperature, whereas Clematis cadmia has a certain temperature adaptability. Here we analyzed the potential regulatory mechanisms of C. crassifolia and C. cad...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034387/ https://www.ncbi.nlm.nih.gov/pubmed/33841457 http://dx.doi.org/10.3389/fpls.2021.624875 |
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author | Hu, Qingdi Qian, Renjuan Zhang, Yanjun Zhang, Xule Ma, Xiaohua Zheng, Jian |
author_facet | Hu, Qingdi Qian, Renjuan Zhang, Yanjun Zhang, Xule Ma, Xiaohua Zheng, Jian |
author_sort | Hu, Qingdi |
collection | PubMed |
description | Clematis is a superior perennial ornamental vine known for varied colors and shapes of its flowers. Clematis crassifolia is sensitive to high temperature, whereas Clematis cadmia has a certain temperature adaptability. Here we analyzed the potential regulatory mechanisms of C. crassifolia and C. cadmia in response to heat stress by studying the photosynthesis, antioxidant parameters, amino acids, and gene expression patterns under three temperature treatments. Heat stress caused the fading of leaves; decreased net photosynthetic rate, stomatal conductance, superoxide dismutase, and catalase activity; increased 13 kinds of amino acids content; and up-regulated the expression of seven genes, including C194329_G3, C194434_G1, and C188817_g1, etc., in C. crassifolia plants. Under the treatments of heat stress, the leaf tips of C. cadmia were wilted, and the net photosynthetic rate and soluble protein content decreased, with the increase of 12 amino acids content and the expression of c194329_g3, c194434_g1, and c195983_g1. Our results showed that C. crassifolia and C. cadmia had different physiological and molecular response mechanisms to heat stress during the ecological adaptation. |
format | Online Article Text |
id | pubmed-8034387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80343872021-04-10 Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress Hu, Qingdi Qian, Renjuan Zhang, Yanjun Zhang, Xule Ma, Xiaohua Zheng, Jian Front Plant Sci Plant Science Clematis is a superior perennial ornamental vine known for varied colors and shapes of its flowers. Clematis crassifolia is sensitive to high temperature, whereas Clematis cadmia has a certain temperature adaptability. Here we analyzed the potential regulatory mechanisms of C. crassifolia and C. cadmia in response to heat stress by studying the photosynthesis, antioxidant parameters, amino acids, and gene expression patterns under three temperature treatments. Heat stress caused the fading of leaves; decreased net photosynthetic rate, stomatal conductance, superoxide dismutase, and catalase activity; increased 13 kinds of amino acids content; and up-regulated the expression of seven genes, including C194329_G3, C194434_G1, and C188817_g1, etc., in C. crassifolia plants. Under the treatments of heat stress, the leaf tips of C. cadmia were wilted, and the net photosynthetic rate and soluble protein content decreased, with the increase of 12 amino acids content and the expression of c194329_g3, c194434_g1, and c195983_g1. Our results showed that C. crassifolia and C. cadmia had different physiological and molecular response mechanisms to heat stress during the ecological adaptation. Frontiers Media S.A. 2021-03-26 /pmc/articles/PMC8034387/ /pubmed/33841457 http://dx.doi.org/10.3389/fpls.2021.624875 Text en Copyright © 2021 Hu, Qian, Zhang, Zhang, Ma and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Hu, Qingdi Qian, Renjuan Zhang, Yanjun Zhang, Xule Ma, Xiaohua Zheng, Jian Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress |
title | Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress |
title_full | Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress |
title_fullStr | Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress |
title_full_unstemmed | Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress |
title_short | Physiological and Gene Expression Changes of Clematis crassifolia and Clematis cadmia in Response to Heat Stress |
title_sort | physiological and gene expression changes of clematis crassifolia and clematis cadmia in response to heat stress |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034387/ https://www.ncbi.nlm.nih.gov/pubmed/33841457 http://dx.doi.org/10.3389/fpls.2021.624875 |
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