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A CRISPR-Cas9–integrase complex generates precise DNA fragments for genome integration

CRISPR-Cas9 is an RNA-guided DNA endonuclease involved in bacterial adaptive immunity and widely repurposed for genome editing in human cells, animals and plants. In bacteria, RNA molecules that guide Cas9′s activity derive from foreign DNA fragments that are captured and integrated into the host CR...

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Detalles Bibliográficos
Autores principales:	Jakhanwal, Shrutee, Cress, Brady F, Maguin, Pascal, Lobba, Marco J, Marraffini, Luciano A, Doudna, Jennifer A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	RNA and RNA-protein complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034620/ https://www.ncbi.nlm.nih.gov/pubmed/33693715 http://dx.doi.org/10.1093/nar/gkab123

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