Cargando…

A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs

Detailed target-selectivity information and experiment-based efficacy prediction tools are primarily available for Streptococcus pyogenes Cas9 (SpCas9). One obstacle to develop such tools is the rarity of accurate data. Here, we report a method termed ‘Self-targeting sgRNA Library Screen’ (SLS) for...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tálas, András, Huszár, Krisztina, Kulcsár, Péter István, Varga, Julia K, Varga, Éva, Tóth, Eszter, Welker, Zsombor, Erdős, Gergely, Pach, Péter Ferenc, Welker, Ágnes, Györgypál, Zoltán, Tusnády, Gábor E, Welker, Ervin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034649/ https://www.ncbi.nlm.nih.gov/pubmed/33450024 http://dx.doi.org/10.1093/nar/gkaa1220

Ejemplares similares

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
por: Kulcsár, Péter István, et al.
Publicado: (2020)

Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs
por: Huszár, Krisztina, et al.
Publicado: (2023)

Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases
por: Tóth, Eszter, et al.
Publicado: (2020)

A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets
por: Kulcsár, Péter István, et al.
Publicado: (2023)

Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells
por: Tóth, Eszter, et al.
Publicado: (2016)

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
por: Kulcsár, Péter István, et al.
Publicado: (2017)

Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning
por: Tóth, Eszter, et al.
Publicado: (2014)

BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
por: Tálas, András, et al.
Publicado: (2021)

Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants
por: Tóth, Eszter, et al.
Publicado: (2018)

SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e
por: Kulcsár, Péter István, et al.
Publicado: (2022)

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells
por: Simon, Dorottya Anna, et al.
Publicado: (2022)

sgRNA-PSM: Predict sgRNAs On-Target Activity Based on Position-Specific Mismatch
por: Liu, Bin, et al.
Publicado: (2020)

A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’ GFP-expression plasmid
por: Tálas, András, et al.
Publicado: (2017)

Efficient large fragment deletion in plants: double pairs of sgRNAs are better than dual sgRNAs
por: Zhu, Guoning, et al.
Publicado: (2023)

CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
por: Moreno-Mateos, Miguel A., et al.
Publicado: (2015)

CRISPRz: a database of zebrafish validated sgRNAs
por: Varshney, Gaurav K., et al.
Publicado: (2016)

Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing
por: Chen, Zexiang, et al.
Publicado: (2022)

CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs
por: Lee, Ho Joung, et al.
Publicado: (2020)

CRISPR-surfaceome: An online tool for designing highly efficient sgRNAs targeting cell surface proteins
por: Mei, Hong, et al.
Publicado: (2022)

Elimination of Specific miRNAs by Naked 14-nt sgRNAs
por: Takahashi, Masayuki, et al.
Publicado: (2012)

CRISPy-web: An online resource to design sgRNAs for CRISPR applications
por: Blin, Kai, et al.
Publicado: (2016)

GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes
por: Merritt, Brian B., et al.
Publicado: (2019)

sgRNAs: A SARS-CoV-2 emerging issue
por: Mori, Antonio, et al.
Publicado: (2023)

CIDP: a multi-functional platform for designing CRISPR sgRNAs
por: Xu, Dong, et al.
Publicado: (2023)

CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems
por: Rahman, Md. Khaledur, et al.
Publicado: (2017)

Evaluating the cleavage efficacy of CRISPR-Cas9 sgRNAs targeting ineffective regions of Arabidopsis thaliana genome
por: Malik, Afsheen, et al.
Publicado: (2021)

CRISPRlnc: a manually curated database of validated sgRNAs for lncRNAs
por: Chen, Wen, et al.
Publicado: (2019)

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
por: Kundert, Kale, et al.
Publicado: (2019)

Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies
por: Hunker, Avery C., et al.
Publicado: (2020)

Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system
por: Yuan, Tingting, et al.
Publicado: (2019)

Artificial sgRNAs engineered for genome editing with new Cas12b orthologs
por: Teng, Fei, et al.
Publicado: (2019)

Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors
por: Zhang, Ao, et al.
Publicado: (2023)

Targeted Knockout of the Vegfa Gene in the Retina by Subretinal Injection of RNP Complexes Containing Cas9 Protein and Modified sgRNAs
por: Holmgaard, Andreas Braae, et al.
Publicado: (2021)

Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton
por: Ramadan, Mohamed, et al.
Publicado: (2021)

Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
por: Liang, Gang, et al.
Publicado: (2016)

Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
por: Kim, Sojung, et al.
Publicado: (2017)

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency
por: Jang, Da Eun, et al.
Publicado: (2018)

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
por: Hegde, Mudra, et al.
Publicado: (2018)

Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency
por: García-Tuñón, Ignacio, et al.
Publicado: (2019)

Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions
por: Blayney, Joseph, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_svcio96ktmh413dfk66m9171ae