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Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry
[Image: see text] Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes. The study of their structure and functions is commonly based on a material that is isolated from complex natural sources. However, a more precise analysis req...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034773/ https://www.ncbi.nlm.nih.gov/pubmed/33667082 http://dx.doi.org/10.1021/acs.analchem.1c00239 |
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author | Nicolardi, Simone Joseph, A. Abragam Zhu, Qian Shen, Zhengnan Pardo-Vargas, Alonso Chiodo, Fabrizio Molinaro, Antonio Silipo, Alba van der Burgt, Yuri E. M. Yu, Biao Seeberger, Peter H. Wuhrer, Manfred |
author_facet | Nicolardi, Simone Joseph, A. Abragam Zhu, Qian Shen, Zhengnan Pardo-Vargas, Alonso Chiodo, Fabrizio Molinaro, Antonio Silipo, Alba van der Burgt, Yuri E. M. Yu, Biao Seeberger, Peter H. Wuhrer, Manfred |
author_sort | Nicolardi, Simone |
collection | PubMed |
description | [Image: see text] Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes. The study of their structure and functions is commonly based on a material that is isolated from complex natural sources. However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses. Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization. Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides. Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures. |
format | Online Article Text |
id | pubmed-8034773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-80347732021-04-13 Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry Nicolardi, Simone Joseph, A. Abragam Zhu, Qian Shen, Zhengnan Pardo-Vargas, Alonso Chiodo, Fabrizio Molinaro, Antonio Silipo, Alba van der Burgt, Yuri E. M. Yu, Biao Seeberger, Peter H. Wuhrer, Manfred Anal Chem [Image: see text] Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes. The study of their structure and functions is commonly based on a material that is isolated from complex natural sources. However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses. Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization. Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides. Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures. American Chemical Society 2021-03-05 2021-03-16 /pmc/articles/PMC8034773/ /pubmed/33667082 http://dx.doi.org/10.1021/acs.analchem.1c00239 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Nicolardi, Simone Joseph, A. Abragam Zhu, Qian Shen, Zhengnan Pardo-Vargas, Alonso Chiodo, Fabrizio Molinaro, Antonio Silipo, Alba van der Burgt, Yuri E. M. Yu, Biao Seeberger, Peter H. Wuhrer, Manfred Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry |
title | Analysis of Synthetic Monodisperse Polysaccharides
by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry |
title_full | Analysis of Synthetic Monodisperse Polysaccharides
by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry |
title_fullStr | Analysis of Synthetic Monodisperse Polysaccharides
by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry |
title_full_unstemmed | Analysis of Synthetic Monodisperse Polysaccharides
by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry |
title_short | Analysis of Synthetic Monodisperse Polysaccharides
by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry |
title_sort | analysis of synthetic monodisperse polysaccharides
by wide mass range ultrahigh-resolution maldi mass spectrometry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034773/ https://www.ncbi.nlm.nih.gov/pubmed/33667082 http://dx.doi.org/10.1021/acs.analchem.1c00239 |
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