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Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy

Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pr...

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Autores principales: Dinić, Miroslav, Herholz, Marija, Kačarević, Uroš, Radojević, Dušan, Novović, Katarina, Đokić, Jelena, Trifunović, Aleksandra, Golić, Nataša
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034897/
https://www.ncbi.nlm.nih.gov/pubmed/33770762
http://dx.doi.org/10.18632/aging.202885
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author Dinić, Miroslav
Herholz, Marija
Kačarević, Uroš
Radojević, Dušan
Novović, Katarina
Đokić, Jelena
Trifunović, Aleksandra
Golić, Nataša
author_facet Dinić, Miroslav
Herholz, Marija
Kačarević, Uroš
Radojević, Dušan
Novović, Katarina
Đokić, Jelena
Trifunović, Aleksandra
Golić, Nataša
author_sort Dinić, Miroslav
collection PubMed
description Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH-30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host.
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spelling pubmed-80348972021-04-16 Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy Dinić, Miroslav Herholz, Marija Kačarević, Uroš Radojević, Dušan Novović, Katarina Đokić, Jelena Trifunović, Aleksandra Golić, Nataša Aging (Albany NY) Research Paper Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH-30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host. Impact Journals 2021-03-26 /pmc/articles/PMC8034897/ /pubmed/33770762 http://dx.doi.org/10.18632/aging.202885 Text en Copyright: © 2021 Dinić et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Dinić, Miroslav
Herholz, Marija
Kačarević, Uroš
Radojević, Dušan
Novović, Katarina
Đokić, Jelena
Trifunović, Aleksandra
Golić, Nataša
Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
title Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
title_full Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
title_fullStr Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
title_full_unstemmed Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
title_short Host–commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
title_sort host–commensal interaction promotes health and lifespan in caenorhabditis elegans through the activation of hlh-30/tfeb-mediated autophagy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034897/
https://www.ncbi.nlm.nih.gov/pubmed/33770762
http://dx.doi.org/10.18632/aging.202885
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