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In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration
Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here, we apply BioID2 proximity labeling to capture protein networks acting within cardiomyo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034980/ https://www.ncbi.nlm.nih.gov/pubmed/33764296 http://dx.doi.org/10.7554/eLife.66079 |
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author | Pronobis, Mira I Zheng, Susan Singh, Sumeet Pal Goldman, Joseph A Poss, Kenneth D |
author_facet | Pronobis, Mira I Zheng, Susan Singh, Sumeet Pal Goldman, Joseph A Poss, Kenneth D |
author_sort | Pronobis, Mira I |
collection | PubMed |
description | Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here, we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa, or vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish. |
format | Online Article Text |
id | pubmed-8034980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80349802021-04-12 In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration Pronobis, Mira I Zheng, Susan Singh, Sumeet Pal Goldman, Joseph A Poss, Kenneth D eLife Developmental Biology Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here, we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa, or vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish. eLife Sciences Publications, Ltd 2021-03-25 /pmc/articles/PMC8034980/ /pubmed/33764296 http://dx.doi.org/10.7554/eLife.66079 Text en © 2021, Pronobis et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Pronobis, Mira I Zheng, Susan Singh, Sumeet Pal Goldman, Joseph A Poss, Kenneth D In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
title | In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
title_full | In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
title_fullStr | In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
title_full_unstemmed | In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
title_short | In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
title_sort | in vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034980/ https://www.ncbi.nlm.nih.gov/pubmed/33764296 http://dx.doi.org/10.7554/eLife.66079 |
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