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circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21
BACKGROUND: There is more and more evidence showed that circRNAs played essentially role in the regulation of various biological processes. The role of circSLC8A1 in prostate cancer (PCa) is yet little known. METHODS: The CircSLC8A1 expression in human prostate cancer was measured by qRT-PCR. The in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035017/ https://www.ncbi.nlm.nih.gov/pubmed/33869627 http://dx.doi.org/10.1155/2021/6614591 |
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author | Wang, Daoyuan Yan, Shuxian Wang, Lihui Li, Yunlong Qiao, Baoping |
author_facet | Wang, Daoyuan Yan, Shuxian Wang, Lihui Li, Yunlong Qiao, Baoping |
author_sort | Wang, Daoyuan |
collection | PubMed |
description | BACKGROUND: There is more and more evidence showed that circRNAs played essentially role in the regulation of various biological processes. The role of circSLC8A1 in prostate cancer (PCa) is yet little known. METHODS: The CircSLC8A1 expression in human prostate cancer was measured by qRT-PCR. The interplay between the specific circRNA, miRNA, and mRNA was investigated by RT-PCR and luciferase reporter assay. Through transient transfection of siRNA, the impacts of circSLC8A1 on PCa were discussed. Cell cycle evaluation, transwell assay, and CCK-8 assay were employed to determine its biological influences. RESULTS: In this study, our data revealed that circSLC8A1 was downregulated in PCa tissues and cells. The reduction of circSLC8A1 resulted in the inhibition of cell proliferation and migration. In mechanism, circSLC8A1 exhibited a direct interaction with miR-21 and displayed as a miRNA sponge to inhibit PCa progression. The functional analysis revealed that the circSLC8A1/miR-21 axis may regulate the cell proliferation, angiogenesis, cell migration, epithelial to mesenchymal transition, MAPK signaling pathway, and chemokine signaling pathway. CONCLUSIONS: CircSLC8A1 functioned as an inhibitor of neoplasm via modulating the miR-21 and might serve as a prospective target for the treatment of PCa. |
format | Online Article Text |
id | pubmed-8035017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-80350172021-04-16 circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 Wang, Daoyuan Yan, Shuxian Wang, Lihui Li, Yunlong Qiao, Baoping Biomed Res Int Research Article BACKGROUND: There is more and more evidence showed that circRNAs played essentially role in the regulation of various biological processes. The role of circSLC8A1 in prostate cancer (PCa) is yet little known. METHODS: The CircSLC8A1 expression in human prostate cancer was measured by qRT-PCR. The interplay between the specific circRNA, miRNA, and mRNA was investigated by RT-PCR and luciferase reporter assay. Through transient transfection of siRNA, the impacts of circSLC8A1 on PCa were discussed. Cell cycle evaluation, transwell assay, and CCK-8 assay were employed to determine its biological influences. RESULTS: In this study, our data revealed that circSLC8A1 was downregulated in PCa tissues and cells. The reduction of circSLC8A1 resulted in the inhibition of cell proliferation and migration. In mechanism, circSLC8A1 exhibited a direct interaction with miR-21 and displayed as a miRNA sponge to inhibit PCa progression. The functional analysis revealed that the circSLC8A1/miR-21 axis may regulate the cell proliferation, angiogenesis, cell migration, epithelial to mesenchymal transition, MAPK signaling pathway, and chemokine signaling pathway. CONCLUSIONS: CircSLC8A1 functioned as an inhibitor of neoplasm via modulating the miR-21 and might serve as a prospective target for the treatment of PCa. Hindawi 2021-04-01 /pmc/articles/PMC8035017/ /pubmed/33869627 http://dx.doi.org/10.1155/2021/6614591 Text en Copyright © 2021 Daoyuan Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Daoyuan Yan, Shuxian Wang, Lihui Li, Yunlong Qiao, Baoping circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 |
title | circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 |
title_full | circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 |
title_fullStr | circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 |
title_full_unstemmed | circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 |
title_short | circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21 |
title_sort | circslc8a1 acts as a tumor suppressor in prostate cancer via sponging mir-21 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035017/ https://www.ncbi.nlm.nih.gov/pubmed/33869627 http://dx.doi.org/10.1155/2021/6614591 |
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