Cargando…

Bark-dwelling methanotrophic bacteria decrease methane emissions from trees

Tree stems are an important and unconstrained source of methane, yet it is uncertain whether internal microbial controls (i.e. methanotrophy) within tree bark may reduce methane emissions. Here we demonstrate that unique microbial communities dominated by methane-oxidising bacteria (MOB) dwell withi...

Descripción completa

Detalles Bibliográficos
Autores principales: Jeffrey, Luke C., Maher, Damien T., Chiri, Eleonora, Leung, Pok Man, Nauer, Philipp A., Arndt, Stefan K., Tait, Douglas R., Greening, Chris, Johnston, Scott G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035153/
https://www.ncbi.nlm.nih.gov/pubmed/33837213
http://dx.doi.org/10.1038/s41467-021-22333-7
_version_ 1783676666920304640
author Jeffrey, Luke C.
Maher, Damien T.
Chiri, Eleonora
Leung, Pok Man
Nauer, Philipp A.
Arndt, Stefan K.
Tait, Douglas R.
Greening, Chris
Johnston, Scott G.
author_facet Jeffrey, Luke C.
Maher, Damien T.
Chiri, Eleonora
Leung, Pok Man
Nauer, Philipp A.
Arndt, Stefan K.
Tait, Douglas R.
Greening, Chris
Johnston, Scott G.
author_sort Jeffrey, Luke C.
collection PubMed
description Tree stems are an important and unconstrained source of methane, yet it is uncertain whether internal microbial controls (i.e. methanotrophy) within tree bark may reduce methane emissions. Here we demonstrate that unique microbial communities dominated by methane-oxidising bacteria (MOB) dwell within bark of Melaleuca quinquenervia, a common, invasive and globally distributed lowland species. In laboratory incubations, methane-inoculated M. quinquenervia bark mediated methane consumption (up to 96.3 µmol m(−2) bark d(−1)) and reveal distinct isotopic δ(13)C-CH(4) enrichment characteristic of MOB. Molecular analysis indicates unique microbial communities reside within the bark, with MOB primarily from the genus Methylomonas comprising up to 25 % of the total microbial community. Methanotroph abundance was linearly correlated to methane uptake rates (R(2) = 0.76, p = 0.006). Finally, field-based methane oxidation inhibition experiments demonstrate that bark-dwelling MOB reduce methane emissions by 36 ± 5 %. These multiple complementary lines of evidence indicate that bark-dwelling MOB represent a potentially significant methane sink, and an important frontier for further research.
format Online
Article
Text
id pubmed-8035153
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-80351532021-04-30 Bark-dwelling methanotrophic bacteria decrease methane emissions from trees Jeffrey, Luke C. Maher, Damien T. Chiri, Eleonora Leung, Pok Man Nauer, Philipp A. Arndt, Stefan K. Tait, Douglas R. Greening, Chris Johnston, Scott G. Nat Commun Article Tree stems are an important and unconstrained source of methane, yet it is uncertain whether internal microbial controls (i.e. methanotrophy) within tree bark may reduce methane emissions. Here we demonstrate that unique microbial communities dominated by methane-oxidising bacteria (MOB) dwell within bark of Melaleuca quinquenervia, a common, invasive and globally distributed lowland species. In laboratory incubations, methane-inoculated M. quinquenervia bark mediated methane consumption (up to 96.3 µmol m(−2) bark d(−1)) and reveal distinct isotopic δ(13)C-CH(4) enrichment characteristic of MOB. Molecular analysis indicates unique microbial communities reside within the bark, with MOB primarily from the genus Methylomonas comprising up to 25 % of the total microbial community. Methanotroph abundance was linearly correlated to methane uptake rates (R(2) = 0.76, p = 0.006). Finally, field-based methane oxidation inhibition experiments demonstrate that bark-dwelling MOB reduce methane emissions by 36 ± 5 %. These multiple complementary lines of evidence indicate that bark-dwelling MOB represent a potentially significant methane sink, and an important frontier for further research. Nature Publishing Group UK 2021-04-09 /pmc/articles/PMC8035153/ /pubmed/33837213 http://dx.doi.org/10.1038/s41467-021-22333-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jeffrey, Luke C.
Maher, Damien T.
Chiri, Eleonora
Leung, Pok Man
Nauer, Philipp A.
Arndt, Stefan K.
Tait, Douglas R.
Greening, Chris
Johnston, Scott G.
Bark-dwelling methanotrophic bacteria decrease methane emissions from trees
title Bark-dwelling methanotrophic bacteria decrease methane emissions from trees
title_full Bark-dwelling methanotrophic bacteria decrease methane emissions from trees
title_fullStr Bark-dwelling methanotrophic bacteria decrease methane emissions from trees
title_full_unstemmed Bark-dwelling methanotrophic bacteria decrease methane emissions from trees
title_short Bark-dwelling methanotrophic bacteria decrease methane emissions from trees
title_sort bark-dwelling methanotrophic bacteria decrease methane emissions from trees
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035153/
https://www.ncbi.nlm.nih.gov/pubmed/33837213
http://dx.doi.org/10.1038/s41467-021-22333-7
work_keys_str_mv AT jeffreylukec barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT maherdamient barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT chirieleonora barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT leungpokman barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT nauerphilippa barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT arndtstefank barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT taitdouglasr barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT greeningchris barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees
AT johnstonscottg barkdwellingmethanotrophicbacteriadecreasemethaneemissionsfromtrees