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Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan

BACKGROUND: Kallar Kahar lake, Punjab, Pakistan is a rich source of phytoplankton which can be used for biofuel production. OBJECTIVE: This study was conducted to investigate the presence of different microalgae species present in this lake and their possible utilization for bioenergy production. MA...

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Autores principales: Ghani, Naila, Shahzadi, Nargis, Sadaf , Sana, Ullah, Inam, Ali, Ehsan, Iqbal, Javed, Rafique, Tanzila, Maqbool, Munazza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035414/
https://www.ncbi.nlm.nih.gov/pubmed/33850937
http://dx.doi.org/10.30498/IJB.2020.122025.2214
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author Ghani, Naila
Shahzadi, Nargis
Sadaf , Sana
Ullah, Inam
Ali, Ehsan
Iqbal, Javed
Rafique, Tanzila
Maqbool, Munazza
author_facet Ghani, Naila
Shahzadi, Nargis
Sadaf , Sana
Ullah, Inam
Ali, Ehsan
Iqbal, Javed
Rafique, Tanzila
Maqbool, Munazza
author_sort Ghani, Naila
collection PubMed
description BACKGROUND: Kallar Kahar lake, Punjab, Pakistan is a rich source of phytoplankton which can be used for biofuel production. OBJECTIVE: This study was conducted to investigate the presence of different microalgae species present in this lake and their possible utilization for bioenergy production. MATERIALS AND METHODS: The crude culture was examined under microscope. Isolation of the identified species was carried out by using serial dilution and colony picking methods. Isolated strains were evaluated by investigating their biomass productivity, salinity resistance and auto-flocculation ability. RESULTS: Four different microalgae species (Chlorella, Scenedesmus, Oscillatoria and Spirulina) were identified in the crude sample. The experimental results indicated that, among the four isolated strains, the Oscillatoria species showed highest biomass productivity (4.2 gL(-1)) and Scenedesmus showed comparatively higher salt resistance. Scenedesmus also showed great potential of auto-flocculation as around 70 % of its cells sediment within 5 h without addition of any external flocculating agent. The lipid content in the isolated strains has also been carried out using Soxhlet extraction. CONCLUSION: Four different microalgae strains have been found in Kallar Kahar lake that reflected good biomass productivity and are capable of auto-flocculation.
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spelling pubmed-80354142021-04-12 Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan Ghani, Naila Shahzadi, Nargis Sadaf , Sana Ullah, Inam Ali, Ehsan Iqbal, Javed Rafique, Tanzila Maqbool, Munazza Iran J Biotechnol Research Article BACKGROUND: Kallar Kahar lake, Punjab, Pakistan is a rich source of phytoplankton which can be used for biofuel production. OBJECTIVE: This study was conducted to investigate the presence of different microalgae species present in this lake and their possible utilization for bioenergy production. MATERIALS AND METHODS: The crude culture was examined under microscope. Isolation of the identified species was carried out by using serial dilution and colony picking methods. Isolated strains were evaluated by investigating their biomass productivity, salinity resistance and auto-flocculation ability. RESULTS: Four different microalgae species (Chlorella, Scenedesmus, Oscillatoria and Spirulina) were identified in the crude sample. The experimental results indicated that, among the four isolated strains, the Oscillatoria species showed highest biomass productivity (4.2 gL(-1)) and Scenedesmus showed comparatively higher salt resistance. Scenedesmus also showed great potential of auto-flocculation as around 70 % of its cells sediment within 5 h without addition of any external flocculating agent. The lipid content in the isolated strains has also been carried out using Soxhlet extraction. CONCLUSION: Four different microalgae strains have been found in Kallar Kahar lake that reflected good biomass productivity and are capable of auto-flocculation. National Institute of Genetic Engineering and Biotechnology 2020 -07-01 /pmc/articles/PMC8035414/ /pubmed/33850937 http://dx.doi.org/10.30498/IJB.2020.122025.2214 Text en Copyright: © 2020 The Author(s); Published by Iranian Journal of Biotechnology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ghani, Naila
Shahzadi, Nargis
Sadaf , Sana
Ullah, Inam
Ali, Ehsan
Iqbal, Javed
Rafique, Tanzila
Maqbool, Munazza
Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan
title Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan
title_full Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan
title_fullStr Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan
title_full_unstemmed Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan
title_short Isolation of Several Indigenous Microalgae from Kallar Kahar Lake, Chakwal Pakistan
title_sort isolation of several indigenous microalgae from kallar kahar lake, chakwal pakistan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035414/
https://www.ncbi.nlm.nih.gov/pubmed/33850937
http://dx.doi.org/10.30498/IJB.2020.122025.2214
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