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Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture

BACKGROUND: Use of avermectin B1b as anthelmintic and insecticidal agent has increased to protect soil and for enhanced crop production. Enhanced production of avermectin B1b was obtained from mutant strain of Streptomyces avermitilis 41445. OBJECTIVES: Modeling of mutant strain S. avermitilis 41445...

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Autores principales: Siddique, Samia, Ashraf Qureshi, Fahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035423/
https://www.ncbi.nlm.nih.gov/pubmed/33850941
http://dx.doi.org/10.30498/IJB.2020.2356
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author Siddique, Samia
Ashraf Qureshi, Fahim
author_facet Siddique, Samia
Ashraf Qureshi, Fahim
author_sort Siddique, Samia
collection PubMed
description BACKGROUND: Use of avermectin B1b as anthelmintic and insecticidal agent has increased to protect soil and for enhanced crop production. Enhanced production of avermectin B1b was obtained from mutant strain of Streptomyces avermitilis 41445. OBJECTIVES: Modeling of mutant strain S. avermitilis 41445 UV 45(m) 3 growth and avermectin B1b production is therefore required for optimization during fermentation process. Kinetics of intracellular avermectin B1b production was studies in shake flask culture during submerged fermentation. MATERIALS AND METHODS: Mathematical models based upon Logistic and Piret Equations have been used to investigate the kinetics of avermectin B1b production and substrate utilization from S. avermitilis 41445 UV 45(m)3. Effect of carbon sources (glucose, maltose, lactose, potato starch, soluble corn starch, and wheat starch), pH (6.0, 6.5, 7.0, and 7.5), agitation speed (150, 200, and 250 rpm) on microbial growth and product formation were evaluated. RESULTS: Maximum avermectin B1b production (420.02mg.L(-1) and cell biomass (31.74 g.L(-1)) were obtained in media having potato starch as carbon substrate at adjusted medium pH 7.5 and 250 rpm agitation speed. Maximum specific growth rate(µ(max)), growth associated avermectin B1b production coefficient (α) and non-growth associated avermectin B1b production coefficient (β) obtained were 0.16h(-1), 0.0 mg.g(-1) and 3.5 mg.g(-1).h(-1) respectively. CONCLUSION: From above results we can conclude that avermectin B1b production is non-growth associated process.
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spelling pubmed-80354232021-04-12 Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture Siddique, Samia Ashraf Qureshi, Fahim Iran J Biotechnol Research Article BACKGROUND: Use of avermectin B1b as anthelmintic and insecticidal agent has increased to protect soil and for enhanced crop production. Enhanced production of avermectin B1b was obtained from mutant strain of Streptomyces avermitilis 41445. OBJECTIVES: Modeling of mutant strain S. avermitilis 41445 UV 45(m) 3 growth and avermectin B1b production is therefore required for optimization during fermentation process. Kinetics of intracellular avermectin B1b production was studies in shake flask culture during submerged fermentation. MATERIALS AND METHODS: Mathematical models based upon Logistic and Piret Equations have been used to investigate the kinetics of avermectin B1b production and substrate utilization from S. avermitilis 41445 UV 45(m)3. Effect of carbon sources (glucose, maltose, lactose, potato starch, soluble corn starch, and wheat starch), pH (6.0, 6.5, 7.0, and 7.5), agitation speed (150, 200, and 250 rpm) on microbial growth and product formation were evaluated. RESULTS: Maximum avermectin B1b production (420.02mg.L(-1) and cell biomass (31.74 g.L(-1)) were obtained in media having potato starch as carbon substrate at adjusted medium pH 7.5 and 250 rpm agitation speed. Maximum specific growth rate(µ(max)), growth associated avermectin B1b production coefficient (α) and non-growth associated avermectin B1b production coefficient (β) obtained were 0.16h(-1), 0.0 mg.g(-1) and 3.5 mg.g(-1).h(-1) respectively. CONCLUSION: From above results we can conclude that avermectin B1b production is non-growth associated process. National Institute of Genetic Engineering and Biotechnology 2020-07-01 /pmc/articles/PMC8035423/ /pubmed/33850941 http://dx.doi.org/10.30498/IJB.2020.2356 Text en Copyright: © 2020 The Author(s); Published by Iranian Journal of Biotechnology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Siddique, Samia
Ashraf Qureshi, Fahim
Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture
title Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture
title_full Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture
title_fullStr Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture
title_full_unstemmed Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture
title_short Kinetics of Avermectin B1b Production by Streptomyces avermitilis 41445 UV 45(m)3 in Shake Flask Culture
title_sort kinetics of avermectin b1b production by streptomyces avermitilis 41445 uv 45(m)3 in shake flask culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035423/
https://www.ncbi.nlm.nih.gov/pubmed/33850941
http://dx.doi.org/10.30498/IJB.2020.2356
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