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Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study

INTRODUCTION: Plasma thrombin generation kinetics as measured by the calibrated automated thrombogram (CAT) assay is a predictor of symptomatic venous thromboembolism after trauma. We hypothesized that data from a new prototype assay for measurement of thrombin generation kinetics in fresh whole blo...

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Autores principales: Ferrara, Michael J., MacArthur, Taleen A., Butenas, Saulius, Mann, Kenneth G., Immermann, Joseph M., Spears, Grant M., Bailey, Kent R., Kozar, Rosemary A., Heller, Stephanie F., Loomis, Erica A., Stephens, Daniel, Park, Myung S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035795/
https://www.ncbi.nlm.nih.gov/pubmed/33870025
http://dx.doi.org/10.1002/rth2.12483
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author Ferrara, Michael J.
MacArthur, Taleen A.
Butenas, Saulius
Mann, Kenneth G.
Immermann, Joseph M.
Spears, Grant M.
Bailey, Kent R.
Kozar, Rosemary A.
Heller, Stephanie F.
Loomis, Erica A.
Stephens, Daniel
Park, Myung S.
author_facet Ferrara, Michael J.
MacArthur, Taleen A.
Butenas, Saulius
Mann, Kenneth G.
Immermann, Joseph M.
Spears, Grant M.
Bailey, Kent R.
Kozar, Rosemary A.
Heller, Stephanie F.
Loomis, Erica A.
Stephens, Daniel
Park, Myung S.
author_sort Ferrara, Michael J.
collection PubMed
description INTRODUCTION: Plasma thrombin generation kinetics as measured by the calibrated automated thrombogram (CAT) assay is a predictor of symptomatic venous thromboembolism after trauma. We hypothesized that data from a new prototype assay for measurement of thrombin generation kinetics in fresh whole blood (near patient testing of thrombin generation), will correlate with the standard CAT assay in the same patients, making it a potential tool in the future care of trauma patients. METHODS: Patients were enrolled from June 2018 to February 2020. Within 12 hours of injury, blood samples were collected simultaneously for both assays. Variables compared and correlated between assays were lag time, peak height, time to peak, and endogenous thrombin potential. Data are presented as median with interquartile range (IQR). Spearman and Pearson correlations were estimated and tested between both assays; a P value of <0.05 was considered to be significant. RESULTS: A total of 64 trauma patients had samples analyzed: injury severity score = 17 (IQR), 10‐26], hospital length of stay = 7.5 (IQR), 2‐18) days, age = 52 (IQR, 35‐63) years, 71.9% male, and 42.2% of patients received a transfusion within 24 hours of injury. Thrombin generation parameters between plasma and whole blood were compared and found that all parameters of the two assays correlate in trauma patients. CONCLUSION: In this pilot study, we have found that a novel point‐of‐care whole blood thrombin generation assay yields results with modest but statistically significant correlations to those of a standard plasma thrombin generation assay. This finding supports studying this device in a larger, adequately powered study.
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spelling pubmed-80357952021-04-15 Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study Ferrara, Michael J. MacArthur, Taleen A. Butenas, Saulius Mann, Kenneth G. Immermann, Joseph M. Spears, Grant M. Bailey, Kent R. Kozar, Rosemary A. Heller, Stephanie F. Loomis, Erica A. Stephens, Daniel Park, Myung S. Res Pract Thromb Haemost Original Articles ‐ Hemostasis INTRODUCTION: Plasma thrombin generation kinetics as measured by the calibrated automated thrombogram (CAT) assay is a predictor of symptomatic venous thromboembolism after trauma. We hypothesized that data from a new prototype assay for measurement of thrombin generation kinetics in fresh whole blood (near patient testing of thrombin generation), will correlate with the standard CAT assay in the same patients, making it a potential tool in the future care of trauma patients. METHODS: Patients were enrolled from June 2018 to February 2020. Within 12 hours of injury, blood samples were collected simultaneously for both assays. Variables compared and correlated between assays were lag time, peak height, time to peak, and endogenous thrombin potential. Data are presented as median with interquartile range (IQR). Spearman and Pearson correlations were estimated and tested between both assays; a P value of <0.05 was considered to be significant. RESULTS: A total of 64 trauma patients had samples analyzed: injury severity score = 17 (IQR), 10‐26], hospital length of stay = 7.5 (IQR), 2‐18) days, age = 52 (IQR, 35‐63) years, 71.9% male, and 42.2% of patients received a transfusion within 24 hours of injury. Thrombin generation parameters between plasma and whole blood were compared and found that all parameters of the two assays correlate in trauma patients. CONCLUSION: In this pilot study, we have found that a novel point‐of‐care whole blood thrombin generation assay yields results with modest but statistically significant correlations to those of a standard plasma thrombin generation assay. This finding supports studying this device in a larger, adequately powered study. John Wiley and Sons Inc. 2021-03-08 /pmc/articles/PMC8035795/ /pubmed/33870025 http://dx.doi.org/10.1002/rth2.12483 Text en © 2021 The Authors. Research and Practice in Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis (ISTH). https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles ‐ Hemostasis
Ferrara, Michael J.
MacArthur, Taleen A.
Butenas, Saulius
Mann, Kenneth G.
Immermann, Joseph M.
Spears, Grant M.
Bailey, Kent R.
Kozar, Rosemary A.
Heller, Stephanie F.
Loomis, Erica A.
Stephens, Daniel
Park, Myung S.
Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study
title Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study
title_full Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study
title_fullStr Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study
title_full_unstemmed Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study
title_short Exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: A pilot study
title_sort exploring the utility of a novel point‐of‐care whole blood thrombin generation assay following trauma: a pilot study
topic Original Articles ‐ Hemostasis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035795/
https://www.ncbi.nlm.nih.gov/pubmed/33870025
http://dx.doi.org/10.1002/rth2.12483
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