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BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)

In ‘Tsuda’ turnip, the swollen root peel accumulates anthocyanin pigments in a light-dependent manner, but the mechanism is unclear. Here, mutant g120w which accumulated extremely low levels of anthocyanin after light exposure was identified. Segregation analysis showed that the anthocyanin-deficien...

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Autores principales: Song, Hyon Dok, Yang, Jianfei, Mun, Nam Hyok, Chen, Bowei, Chen, Yunzhu, Kim, Pyol, Kawabata, Saneyuki, Li, Yuhua, Wang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036442/
https://www.ncbi.nlm.nih.gov/pubmed/33805479
http://dx.doi.org/10.3390/ijms22073538
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author Song, Hyon Dok
Yang, Jianfei
Mun, Nam Hyok
Chen, Bowei
Chen, Yunzhu
Kim, Pyol
Kawabata, Saneyuki
Li, Yuhua
Wang, Yu
author_facet Song, Hyon Dok
Yang, Jianfei
Mun, Nam Hyok
Chen, Bowei
Chen, Yunzhu
Kim, Pyol
Kawabata, Saneyuki
Li, Yuhua
Wang, Yu
author_sort Song, Hyon Dok
collection PubMed
description In ‘Tsuda’ turnip, the swollen root peel accumulates anthocyanin pigments in a light-dependent manner, but the mechanism is unclear. Here, mutant g120w which accumulated extremely low levels of anthocyanin after light exposure was identified. Segregation analysis showed that the anthocyanin-deficient phenotype was controlled by a single recessive gene. By using bulked-segregant analysis sequencing and CAPS marker-based genetic mapping analyses, a 21.6-kb region on chromosome A07 was mapped, in which a calcium-binding EF hand family protein named BrLETM2 was identified as the causal gene. RNA sequencing analysis showed that differentially expressed genes (DEGs) between wild type and g120w in light-exposed swollen root peels were enriched in anthocyanin biosynthetic process and reactive oxygen species (ROS) biosynthetic process GO term. Furthermore, nitroblue tetrazolium (NBT) staining showed that the ROS level decreased in g120w mutant. Anthocyanins induced by UV-A were abolished by the pre-treatment of seedlings with DPI (an inhibitor of nicotinamide adenine nucleoside phosphorylase (NADPH) oxidase) and decreased in g120w mutant. These results indicate that BrLETM2 modulates ROS signaling to promote anthocyanin accumulation in turnip under UV-A and provides new insight into the mechanism of how ROS and light regulate anthocyanin production.
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spelling pubmed-80364422021-04-12 BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa) Song, Hyon Dok Yang, Jianfei Mun, Nam Hyok Chen, Bowei Chen, Yunzhu Kim, Pyol Kawabata, Saneyuki Li, Yuhua Wang, Yu Int J Mol Sci Article In ‘Tsuda’ turnip, the swollen root peel accumulates anthocyanin pigments in a light-dependent manner, but the mechanism is unclear. Here, mutant g120w which accumulated extremely low levels of anthocyanin after light exposure was identified. Segregation analysis showed that the anthocyanin-deficient phenotype was controlled by a single recessive gene. By using bulked-segregant analysis sequencing and CAPS marker-based genetic mapping analyses, a 21.6-kb region on chromosome A07 was mapped, in which a calcium-binding EF hand family protein named BrLETM2 was identified as the causal gene. RNA sequencing analysis showed that differentially expressed genes (DEGs) between wild type and g120w in light-exposed swollen root peels were enriched in anthocyanin biosynthetic process and reactive oxygen species (ROS) biosynthetic process GO term. Furthermore, nitroblue tetrazolium (NBT) staining showed that the ROS level decreased in g120w mutant. Anthocyanins induced by UV-A were abolished by the pre-treatment of seedlings with DPI (an inhibitor of nicotinamide adenine nucleoside phosphorylase (NADPH) oxidase) and decreased in g120w mutant. These results indicate that BrLETM2 modulates ROS signaling to promote anthocyanin accumulation in turnip under UV-A and provides new insight into the mechanism of how ROS and light regulate anthocyanin production. MDPI 2021-03-29 /pmc/articles/PMC8036442/ /pubmed/33805479 http://dx.doi.org/10.3390/ijms22073538 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Song, Hyon Dok
Yang, Jianfei
Mun, Nam Hyok
Chen, Bowei
Chen, Yunzhu
Kim, Pyol
Kawabata, Saneyuki
Li, Yuhua
Wang, Yu
BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)
title BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)
title_full BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)
title_fullStr BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)
title_full_unstemmed BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)
title_short BrLETM2 Protein Modulates Anthocyanin Accumulation by Promoting ROS Production in Turnip (Brassica rapa subsp. rapa)
title_sort brletm2 protein modulates anthocyanin accumulation by promoting ros production in turnip (brassica rapa subsp. rapa)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036442/
https://www.ncbi.nlm.nih.gov/pubmed/33805479
http://dx.doi.org/10.3390/ijms22073538
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