Cargando…

In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats

The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of the rat lumbar spinal cord and plays a pivotal role in the ejaculatory reflex. We recently reported that SEG neurons express the oxytocin receptor and are activated by oxytocin projections from the paraventricular nu...

Descripción completa

Detalles Bibliográficos
Autores principales: Uta, Daisuke, Oti, Takumi, Sakamoto, Tatsuya, Sakamoto, Hirotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036474/
https://www.ncbi.nlm.nih.gov/pubmed/33810239
http://dx.doi.org/10.3390/ijms22073400
_version_ 1783676918616293376
author Uta, Daisuke
Oti, Takumi
Sakamoto, Tatsuya
Sakamoto, Hirotaka
author_facet Uta, Daisuke
Oti, Takumi
Sakamoto, Tatsuya
Sakamoto, Hirotaka
author_sort Uta, Daisuke
collection PubMed
description The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of the rat lumbar spinal cord and plays a pivotal role in the ejaculatory reflex. We recently reported that SEG neurons express the oxytocin receptor and are activated by oxytocin projections from the paraventricular nucleus of hypothalamus (PVH). However, it is unknown whether the SEG responds to oxytocin in vivo. In this study, we analyzed the characteristics of the brain–spinal cord neural circuit that controls male sexual function using a newly developed in vivo electrophysiological technique. Optogenetic stimulation of the PVH of rats expressing channel rhodopsin under the oxytocin receptor promoter increased the spontaneous firing of most lamina X SEG neurons. This is the first demonstration of the in vivo electrical response from the deeper (lamina X) neurons in the spinal cord. Furthermore, we succeeded in the in vivo whole-cell recordings of lamina X neurons. In vivo whole-cell recordings may reveal the features of lamina X SEG neurons, including differences in neurotransmitters and response to stimulation. Taken together, these results suggest that in vivo electrophysiological stimulation can elucidate the neurophysiological response of a variety of spinal neurons during male sexual behavior.
format Online
Article
Text
id pubmed-8036474
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80364742021-04-12 In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats Uta, Daisuke Oti, Takumi Sakamoto, Tatsuya Sakamoto, Hirotaka Int J Mol Sci Article The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of the rat lumbar spinal cord and plays a pivotal role in the ejaculatory reflex. We recently reported that SEG neurons express the oxytocin receptor and are activated by oxytocin projections from the paraventricular nucleus of hypothalamus (PVH). However, it is unknown whether the SEG responds to oxytocin in vivo. In this study, we analyzed the characteristics of the brain–spinal cord neural circuit that controls male sexual function using a newly developed in vivo electrophysiological technique. Optogenetic stimulation of the PVH of rats expressing channel rhodopsin under the oxytocin receptor promoter increased the spontaneous firing of most lamina X SEG neurons. This is the first demonstration of the in vivo electrical response from the deeper (lamina X) neurons in the spinal cord. Furthermore, we succeeded in the in vivo whole-cell recordings of lamina X neurons. In vivo whole-cell recordings may reveal the features of lamina X SEG neurons, including differences in neurotransmitters and response to stimulation. Taken together, these results suggest that in vivo electrophysiological stimulation can elucidate the neurophysiological response of a variety of spinal neurons during male sexual behavior. MDPI 2021-03-26 /pmc/articles/PMC8036474/ /pubmed/33810239 http://dx.doi.org/10.3390/ijms22073400 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Uta, Daisuke
Oti, Takumi
Sakamoto, Tatsuya
Sakamoto, Hirotaka
In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats
title In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats
title_full In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats
title_fullStr In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats
title_full_unstemmed In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats
title_short In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats
title_sort in vivo electrophysiology of peptidergic neurons in deep layers of the lumbar spinal cord after optogenetic stimulation of hypothalamic paraventricular oxytocin neurons in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036474/
https://www.ncbi.nlm.nih.gov/pubmed/33810239
http://dx.doi.org/10.3390/ijms22073400
work_keys_str_mv AT utadaisuke invivoelectrophysiologyofpeptidergicneuronsindeeplayersofthelumbarspinalcordafteroptogeneticstimulationofhypothalamicparaventricularoxytocinneuronsinrats
AT otitakumi invivoelectrophysiologyofpeptidergicneuronsindeeplayersofthelumbarspinalcordafteroptogeneticstimulationofhypothalamicparaventricularoxytocinneuronsinrats
AT sakamototatsuya invivoelectrophysiologyofpeptidergicneuronsindeeplayersofthelumbarspinalcordafteroptogeneticstimulationofhypothalamicparaventricularoxytocinneuronsinrats
AT sakamotohirotaka invivoelectrophysiologyofpeptidergicneuronsindeeplayersofthelumbarspinalcordafteroptogeneticstimulationofhypothalamicparaventricularoxytocinneuronsinrats