Cargando…
Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed f...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036500/ https://www.ncbi.nlm.nih.gov/pubmed/33805139 http://dx.doi.org/10.3390/ijerph18073372 |
_version_ | 1783676924707471360 |
---|---|
author | Nyabi, Omar Bentahir, Mostafa Ambroise, Jérôme Bearzatto, Bertrand Chibani, Nawfal Smits, Benjamin Durant, Jean François Vybornov, Aleksandr Thellin, Olivier El Moualij, Benaissa Gala, Jean-Luc |
author_facet | Nyabi, Omar Bentahir, Mostafa Ambroise, Jérôme Bearzatto, Bertrand Chibani, Nawfal Smits, Benjamin Durant, Jean François Vybornov, Aleksandr Thellin, Olivier El Moualij, Benaissa Gala, Jean-Luc |
author_sort | Nyabi, Omar |
collection | PubMed |
description | Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed for the surveillance of COVID-19 cases by large-scale testing of first responders. The objective was to assess the seroconversion among the regional civil protection (CP), police, health care professionals, and volunteers. The secondary objective was to detect asymptomatic individuals within this cohort in the light of age, sex, and residence. In this paper, we report the results of serological testing performed by the B-LiFe mobile laboratory deployed from 10 June to 23 July 2020. The tests included whole blood finger-prick and serum sampling for detection of SARS-CoV-2 spike receptor-binding domain (S-RBD) antibodies. The prevalence of SARS-CoV-2 antibodies was approximately 5% (294/6013). The results of the finger-prick tests and serum sample analyses showed moderate agreement (kappa = 0.77). Furthermore, the detection rates of serum antibodies to the SARS-CoV-2 nucleocapsid protein (NP) and S-RBD among the seroconverted individuals were positively correlated (kappa = 0.60), at least at the IgG level. Seroprevalence studies based on serological testing for the S-RBD protein or SARS-CoV-2 NP antibodies are not sufficient for diagnosis but might help in screening the population to be vaccinated and in determining the duration of seroconversion. |
format | Online Article Text |
id | pubmed-8036500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80365002021-04-12 Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region Nyabi, Omar Bentahir, Mostafa Ambroise, Jérôme Bearzatto, Bertrand Chibani, Nawfal Smits, Benjamin Durant, Jean François Vybornov, Aleksandr Thellin, Olivier El Moualij, Benaissa Gala, Jean-Luc Int J Environ Res Public Health Article Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed for the surveillance of COVID-19 cases by large-scale testing of first responders. The objective was to assess the seroconversion among the regional civil protection (CP), police, health care professionals, and volunteers. The secondary objective was to detect asymptomatic individuals within this cohort in the light of age, sex, and residence. In this paper, we report the results of serological testing performed by the B-LiFe mobile laboratory deployed from 10 June to 23 July 2020. The tests included whole blood finger-prick and serum sampling for detection of SARS-CoV-2 spike receptor-binding domain (S-RBD) antibodies. The prevalence of SARS-CoV-2 antibodies was approximately 5% (294/6013). The results of the finger-prick tests and serum sample analyses showed moderate agreement (kappa = 0.77). Furthermore, the detection rates of serum antibodies to the SARS-CoV-2 nucleocapsid protein (NP) and S-RBD among the seroconverted individuals were positively correlated (kappa = 0.60), at least at the IgG level. Seroprevalence studies based on serological testing for the S-RBD protein or SARS-CoV-2 NP antibodies are not sufficient for diagnosis but might help in screening the population to be vaccinated and in determining the duration of seroconversion. MDPI 2021-03-24 /pmc/articles/PMC8036500/ /pubmed/33805139 http://dx.doi.org/10.3390/ijerph18073372 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Nyabi, Omar Bentahir, Mostafa Ambroise, Jérôme Bearzatto, Bertrand Chibani, Nawfal Smits, Benjamin Durant, Jean François Vybornov, Aleksandr Thellin, Olivier El Moualij, Benaissa Gala, Jean-Luc Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region |
title | Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region |
title_full | Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region |
title_fullStr | Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region |
title_full_unstemmed | Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region |
title_short | Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region |
title_sort | diagnostic value of igm and igg detection in covid-19 diagnosis by the mobile laboratory b-life: a massive testing strategy in the piedmont region |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036500/ https://www.ncbi.nlm.nih.gov/pubmed/33805139 http://dx.doi.org/10.3390/ijerph18073372 |
work_keys_str_mv | AT nyabiomar diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT bentahirmostafa diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT ambroisejerome diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT bearzattobertrand diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT chibaninawfal diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT smitsbenjamin diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT durantjeanfrancois diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT vybornovaleksandr diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT thellinolivier diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT elmoualijbenaissa diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion AT galajeanluc diagnosticvalueofigmandiggdetectionincovid19diagnosisbythemobilelaboratoryblifeamassivetestingstrategyinthepiedmontregion |