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Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region

Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed f...

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Autores principales: Nyabi, Omar, Bentahir, Mostafa, Ambroise, Jérôme, Bearzatto, Bertrand, Chibani, Nawfal, Smits, Benjamin, Durant, Jean François, Vybornov, Aleksandr, Thellin, Olivier, El Moualij, Benaissa, Gala, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036500/
https://www.ncbi.nlm.nih.gov/pubmed/33805139
http://dx.doi.org/10.3390/ijerph18073372
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author Nyabi, Omar
Bentahir, Mostafa
Ambroise, Jérôme
Bearzatto, Bertrand
Chibani, Nawfal
Smits, Benjamin
Durant, Jean François
Vybornov, Aleksandr
Thellin, Olivier
El Moualij, Benaissa
Gala, Jean-Luc
author_facet Nyabi, Omar
Bentahir, Mostafa
Ambroise, Jérôme
Bearzatto, Bertrand
Chibani, Nawfal
Smits, Benjamin
Durant, Jean François
Vybornov, Aleksandr
Thellin, Olivier
El Moualij, Benaissa
Gala, Jean-Luc
author_sort Nyabi, Omar
collection PubMed
description Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed for the surveillance of COVID-19 cases by large-scale testing of first responders. The objective was to assess the seroconversion among the regional civil protection (CP), police, health care professionals, and volunteers. The secondary objective was to detect asymptomatic individuals within this cohort in the light of age, sex, and residence. In this paper, we report the results of serological testing performed by the B-LiFe mobile laboratory deployed from 10 June to 23 July 2020. The tests included whole blood finger-prick and serum sampling for detection of SARS-CoV-2 spike receptor-binding domain (S-RBD) antibodies. The prevalence of SARS-CoV-2 antibodies was approximately 5% (294/6013). The results of the finger-prick tests and serum sample analyses showed moderate agreement (kappa = 0.77). Furthermore, the detection rates of serum antibodies to the SARS-CoV-2 nucleocapsid protein (NP) and S-RBD among the seroconverted individuals were positively correlated (kappa = 0.60), at least at the IgG level. Seroprevalence studies based on serological testing for the S-RBD protein or SARS-CoV-2 NP antibodies are not sufficient for diagnosis but might help in screening the population to be vaccinated and in determining the duration of seroconversion.
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spelling pubmed-80365002021-04-12 Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region Nyabi, Omar Bentahir, Mostafa Ambroise, Jérôme Bearzatto, Bertrand Chibani, Nawfal Smits, Benjamin Durant, Jean François Vybornov, Aleksandr Thellin, Olivier El Moualij, Benaissa Gala, Jean-Luc Int J Environ Res Public Health Article Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed for the surveillance of COVID-19 cases by large-scale testing of first responders. The objective was to assess the seroconversion among the regional civil protection (CP), police, health care professionals, and volunteers. The secondary objective was to detect asymptomatic individuals within this cohort in the light of age, sex, and residence. In this paper, we report the results of serological testing performed by the B-LiFe mobile laboratory deployed from 10 June to 23 July 2020. The tests included whole blood finger-prick and serum sampling for detection of SARS-CoV-2 spike receptor-binding domain (S-RBD) antibodies. The prevalence of SARS-CoV-2 antibodies was approximately 5% (294/6013). The results of the finger-prick tests and serum sample analyses showed moderate agreement (kappa = 0.77). Furthermore, the detection rates of serum antibodies to the SARS-CoV-2 nucleocapsid protein (NP) and S-RBD among the seroconverted individuals were positively correlated (kappa = 0.60), at least at the IgG level. Seroprevalence studies based on serological testing for the S-RBD protein or SARS-CoV-2 NP antibodies are not sufficient for diagnosis but might help in screening the population to be vaccinated and in determining the duration of seroconversion. MDPI 2021-03-24 /pmc/articles/PMC8036500/ /pubmed/33805139 http://dx.doi.org/10.3390/ijerph18073372 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Nyabi, Omar
Bentahir, Mostafa
Ambroise, Jérôme
Bearzatto, Bertrand
Chibani, Nawfal
Smits, Benjamin
Durant, Jean François
Vybornov, Aleksandr
Thellin, Olivier
El Moualij, Benaissa
Gala, Jean-Luc
Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
title Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
title_full Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
title_fullStr Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
title_full_unstemmed Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
title_short Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
title_sort diagnostic value of igm and igg detection in covid-19 diagnosis by the mobile laboratory b-life: a massive testing strategy in the piedmont region
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036500/
https://www.ncbi.nlm.nih.gov/pubmed/33805139
http://dx.doi.org/10.3390/ijerph18073372
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