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An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa

Momordica balsamina leaf extracts originating from three different geographical locations were analyzed using reversed-phase liquid chromatography (RP-LC) coupled to travelling wave ion mobility (TWIMS) and high-resolution mass spectrometry (HRMS) in conjunction with chemometric analysis to differen...

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Autores principales: Venter, Pieter, Malemela, Kholofelo, Mbazima, Vusi, Mampuru, Leseilane J., Muller, Christo J. F., Riedel, Sylvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036689/
https://www.ncbi.nlm.nih.gov/pubmed/33801575
http://dx.doi.org/10.3390/molecules26071896
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author Venter, Pieter
Malemela, Kholofelo
Mbazima, Vusi
Mampuru, Leseilane J.
Muller, Christo J. F.
Riedel, Sylvia
author_facet Venter, Pieter
Malemela, Kholofelo
Mbazima, Vusi
Mampuru, Leseilane J.
Muller, Christo J. F.
Riedel, Sylvia
author_sort Venter, Pieter
collection PubMed
description Momordica balsamina leaf extracts originating from three different geographical locations were analyzed using reversed-phase liquid chromatography (RP-LC) coupled to travelling wave ion mobility (TWIMS) and high-resolution mass spectrometry (HRMS) in conjunction with chemometric analysis to differentiate between potential chemotypes. Furthermore, the cytotoxicity of the three individual chemotypes was evaluated using HT-29 colon cancer cells. A total of 11 molecular species including three flavonol glycosides, five cucurbitane-type triterpenoid aglycones and three glycosidic cucurbitane-type triterpenoids were identified. The cucurbitane-type triterpenoid aglycones were detected in the positive ionization mode following dehydration [M + H − H(2)O](+) of the parent compound, whereas the cucurbitane-type triterpenoid glycosides were primarily identified following adduct formation with ammonia [M + NH(4)](+). The principle component analysis (PCA) loadings plot and a variable influence on projection (VIP) analysis revealed that the isomeric pair balsaminol E and/or karavilagen E was the key molecular species contributing to the distinction between geographical samples. Ultimately, based on statistical analysis, it is hypothesized that balsaminol E and/or karavilagen E are likely responsible for the cytotoxic effects in HT-29 cells.
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spelling pubmed-80366892021-04-12 An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa Venter, Pieter Malemela, Kholofelo Mbazima, Vusi Mampuru, Leseilane J. Muller, Christo J. F. Riedel, Sylvia Molecules Article Momordica balsamina leaf extracts originating from three different geographical locations were analyzed using reversed-phase liquid chromatography (RP-LC) coupled to travelling wave ion mobility (TWIMS) and high-resolution mass spectrometry (HRMS) in conjunction with chemometric analysis to differentiate between potential chemotypes. Furthermore, the cytotoxicity of the three individual chemotypes was evaluated using HT-29 colon cancer cells. A total of 11 molecular species including three flavonol glycosides, five cucurbitane-type triterpenoid aglycones and three glycosidic cucurbitane-type triterpenoids were identified. The cucurbitane-type triterpenoid aglycones were detected in the positive ionization mode following dehydration [M + H − H(2)O](+) of the parent compound, whereas the cucurbitane-type triterpenoid glycosides were primarily identified following adduct formation with ammonia [M + NH(4)](+). The principle component analysis (PCA) loadings plot and a variable influence on projection (VIP) analysis revealed that the isomeric pair balsaminol E and/or karavilagen E was the key molecular species contributing to the distinction between geographical samples. Ultimately, based on statistical analysis, it is hypothesized that balsaminol E and/or karavilagen E are likely responsible for the cytotoxic effects in HT-29 cells. MDPI 2021-03-27 /pmc/articles/PMC8036689/ /pubmed/33801575 http://dx.doi.org/10.3390/molecules26071896 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Venter, Pieter
Malemela, Kholofelo
Mbazima, Vusi
Mampuru, Leseilane J.
Muller, Christo J. F.
Riedel, Sylvia
An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa
title An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa
title_full An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa
title_fullStr An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa
title_full_unstemmed An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa
title_short An RP-LC-UV-TWIMS-HRMS and Chemometric Approach to Differentiate between Momordica balsamina Chemotypes from Three Different Geographical Locations in Limpopo Province of South Africa
title_sort rp-lc-uv-twims-hrms and chemometric approach to differentiate between momordica balsamina chemotypes from three different geographical locations in limpopo province of south africa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036689/
https://www.ncbi.nlm.nih.gov/pubmed/33801575
http://dx.doi.org/10.3390/molecules26071896
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