Cargando…

Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion

Poly(hydroxyalkanoates) (PHAs) with differing material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), with a 3HV content of 25 wt.% and a medium chain length PHA, and mcl-PHA, mainly composed of 3-hydrox...

Descripción completa

Detalles Bibliográficos
Autores principales: Esmail, Asiyah, Pereira, João R., Zoio, Patrícia, Silvestre, Sara, Menda, Ugur Deneb, Sevrin, Chantal, Grandfils, Christian, Fortunato, Elvira, Reis, Maria A. M., Henriques, Célia, Oliva, Abel, Freitas, Filomena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036702/
https://www.ncbi.nlm.nih.gov/pubmed/33801747
http://dx.doi.org/10.3390/polym13071056
_version_ 1783676972069552128
author Esmail, Asiyah
Pereira, João R.
Zoio, Patrícia
Silvestre, Sara
Menda, Ugur Deneb
Sevrin, Chantal
Grandfils, Christian
Fortunato, Elvira
Reis, Maria A. M.
Henriques, Célia
Oliva, Abel
Freitas, Filomena
author_facet Esmail, Asiyah
Pereira, João R.
Zoio, Patrícia
Silvestre, Sara
Menda, Ugur Deneb
Sevrin, Chantal
Grandfils, Christian
Fortunato, Elvira
Reis, Maria A. M.
Henriques, Célia
Oliva, Abel
Freitas, Filomena
author_sort Esmail, Asiyah
collection PubMed
description Poly(hydroxyalkanoates) (PHAs) with differing material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), with a 3HV content of 25 wt.% and a medium chain length PHA, and mcl-PHA, mainly composed of 3-hydroxydecanoate, were studied as scaffolding material for cell culture. P(3HB) and P(3HB-co-3HV) were individually spun into fibers, as well as blends of the mcl-PHA with each of the scl-PHAs. An overall biopolymer concentration of 4 wt.% was used to prepare the electrospinning solutions, using chloroform as the solvent. A stable electrospinning process and good quality fibers were obtained for a solution flow rate of 0.5 mL h(−1), a needle tip collector distance of 20 cm and a voltage of 12 kV for P(3HB) and P(3HB-co-3HV) solutions, while for the mcl-PHA the distance was increased to 25 cm and the voltage to 15 kV. The scaffolds’ hydrophilicity was significantly increased under exposure to oxygen plasma as a surface treatment. Complete wetting was obtained for the oxygen plasma treated scaffolds and the water uptake degree increased in all treated scaffolds. The biopolymers crystallinity was not affected by the electrospinning process, while their treatment with oxygen plasma decreased their crystalline fraction. Human dermal fibroblasts were able to adhere and proliferate within the electrospun PHA-based scaffolds. The P(3HB-co-3HV): mcl-PHA oxygen plasma treated scaffold highlighted the most promising results with a cell adhesion rate of 40 ± 8%, compared to 14 ± 4% for the commercial oxygen plasma treated polystyrene scaffold Alvetex(TM). Scaffolds based on P(3HB-co-3HV): mcl-PHA blends produced by electrospinning and submitted to oxygen plasma exposure are therefore promising biomaterials for the development of scaffolds for tissue engineering.
format Online
Article
Text
id pubmed-8036702
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80367022021-04-12 Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion Esmail, Asiyah Pereira, João R. Zoio, Patrícia Silvestre, Sara Menda, Ugur Deneb Sevrin, Chantal Grandfils, Christian Fortunato, Elvira Reis, Maria A. M. Henriques, Célia Oliva, Abel Freitas, Filomena Polymers (Basel) Article Poly(hydroxyalkanoates) (PHAs) with differing material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), with a 3HV content of 25 wt.% and a medium chain length PHA, and mcl-PHA, mainly composed of 3-hydroxydecanoate, were studied as scaffolding material for cell culture. P(3HB) and P(3HB-co-3HV) were individually spun into fibers, as well as blends of the mcl-PHA with each of the scl-PHAs. An overall biopolymer concentration of 4 wt.% was used to prepare the electrospinning solutions, using chloroform as the solvent. A stable electrospinning process and good quality fibers were obtained for a solution flow rate of 0.5 mL h(−1), a needle tip collector distance of 20 cm and a voltage of 12 kV for P(3HB) and P(3HB-co-3HV) solutions, while for the mcl-PHA the distance was increased to 25 cm and the voltage to 15 kV. The scaffolds’ hydrophilicity was significantly increased under exposure to oxygen plasma as a surface treatment. Complete wetting was obtained for the oxygen plasma treated scaffolds and the water uptake degree increased in all treated scaffolds. The biopolymers crystallinity was not affected by the electrospinning process, while their treatment with oxygen plasma decreased their crystalline fraction. Human dermal fibroblasts were able to adhere and proliferate within the electrospun PHA-based scaffolds. The P(3HB-co-3HV): mcl-PHA oxygen plasma treated scaffold highlighted the most promising results with a cell adhesion rate of 40 ± 8%, compared to 14 ± 4% for the commercial oxygen plasma treated polystyrene scaffold Alvetex(TM). Scaffolds based on P(3HB-co-3HV): mcl-PHA blends produced by electrospinning and submitted to oxygen plasma exposure are therefore promising biomaterials for the development of scaffolds for tissue engineering. MDPI 2021-03-27 /pmc/articles/PMC8036702/ /pubmed/33801747 http://dx.doi.org/10.3390/polym13071056 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Esmail, Asiyah
Pereira, João R.
Zoio, Patrícia
Silvestre, Sara
Menda, Ugur Deneb
Sevrin, Chantal
Grandfils, Christian
Fortunato, Elvira
Reis, Maria A. M.
Henriques, Célia
Oliva, Abel
Freitas, Filomena
Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion
title Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion
title_full Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion
title_fullStr Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion
title_full_unstemmed Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion
title_short Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion
title_sort oxygen plasma treated-electrospun polyhydroxyalkanoate scaffolds for hydrophilicity improvement and cell adhesion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036702/
https://www.ncbi.nlm.nih.gov/pubmed/33801747
http://dx.doi.org/10.3390/polym13071056
work_keys_str_mv AT esmailasiyah oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT pereirajoaor oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT zoiopatricia oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT silvestresara oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT mendaugurdeneb oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT sevrinchantal oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT grandfilschristian oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT fortunatoelvira oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT reismariaam oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT henriquescelia oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT olivaabel oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion
AT freitasfilomena oxygenplasmatreatedelectrospunpolyhydroxyalkanoatescaffoldsforhydrophilicityimprovementandcelladhesion