Cargando…
Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. St...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037002/ https://www.ncbi.nlm.nih.gov/pubmed/33806219 http://dx.doi.org/10.3390/ijms22073391 |
_version_ | 1783677041797758976 |
---|---|
author | Grabska-Zielińska, Sylwia Sionkowska, Alina Olewnik-Kruszkowska, Ewa Reczyńska, Katarzyna Pamuła, Elżbieta |
author_facet | Grabska-Zielińska, Sylwia Sionkowska, Alina Olewnik-Kruszkowska, Ewa Reczyńska, Katarzyna Pamuła, Elżbieta |
author_sort | Grabska-Zielińska, Sylwia |
collection | PubMed |
description | The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm(3); cross-linked with DAC: 14.6 ± 0.7 g/cm(3)) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects. |
format | Online Article Text |
id | pubmed-8037002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80370022021-04-12 Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? Grabska-Zielińska, Sylwia Sionkowska, Alina Olewnik-Kruszkowska, Ewa Reczyńska, Katarzyna Pamuła, Elżbieta Int J Mol Sci Article The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm(3); cross-linked with DAC: 14.6 ± 0.7 g/cm(3)) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects. MDPI 2021-03-25 /pmc/articles/PMC8037002/ /pubmed/33806219 http://dx.doi.org/10.3390/ijms22073391 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Grabska-Zielińska, Sylwia Sionkowska, Alina Olewnik-Kruszkowska, Ewa Reczyńska, Katarzyna Pamuła, Elżbieta Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? |
title | Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? |
title_full | Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? |
title_fullStr | Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? |
title_full_unstemmed | Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? |
title_short | Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials? |
title_sort | is dialdehyde chitosan a good substance to modify physicochemical properties of biopolymeric materials? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037002/ https://www.ncbi.nlm.nih.gov/pubmed/33806219 http://dx.doi.org/10.3390/ijms22073391 |
work_keys_str_mv | AT grabskazielinskasylwia isdialdehydechitosanagoodsubstancetomodifyphysicochemicalpropertiesofbiopolymericmaterials AT sionkowskaalina isdialdehydechitosanagoodsubstancetomodifyphysicochemicalpropertiesofbiopolymericmaterials AT olewnikkruszkowskaewa isdialdehydechitosanagoodsubstancetomodifyphysicochemicalpropertiesofbiopolymericmaterials AT reczynskakatarzyna isdialdehydechitosanagoodsubstancetomodifyphysicochemicalpropertiesofbiopolymericmaterials AT pamułaelzbieta isdialdehydechitosanagoodsubstancetomodifyphysicochemicalpropertiesofbiopolymericmaterials |