Cargando…

Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts

Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel si...

Descripción completa

Detalles Bibliográficos
Autores principales: Toledano-Osorio, Manuel, Manzano-Moreno, Francisco J., Toledano, Manuel, Medina-Castillo, Antonio L., Costela-Ruiz, Victor J., Ruiz, Concepción, Osorio, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037272/
https://www.ncbi.nlm.nih.gov/pubmed/33800569
http://dx.doi.org/10.3390/polym13071063
_version_ 1783677104769990656
author Toledano-Osorio, Manuel
Manzano-Moreno, Francisco J.
Toledano, Manuel
Medina-Castillo, Antonio L.
Costela-Ruiz, Victor J.
Ruiz, Concepción
Osorio, Raquel
author_facet Toledano-Osorio, Manuel
Manzano-Moreno, Francisco J.
Toledano, Manuel
Medina-Castillo, Antonio L.
Costela-Ruiz, Victor J.
Ruiz, Concepción
Osorio, Raquel
author_sort Toledano-Osorio, Manuel
collection PubMed
description Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts’ proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease.
format Online
Article
Text
id pubmed-8037272
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80372722021-04-12 Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts Toledano-Osorio, Manuel Manzano-Moreno, Francisco J. Toledano, Manuel Medina-Castillo, Antonio L. Costela-Ruiz, Victor J. Ruiz, Concepción Osorio, Raquel Polymers (Basel) Article Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts’ proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease. MDPI 2021-03-28 /pmc/articles/PMC8037272/ /pubmed/33800569 http://dx.doi.org/10.3390/polym13071063 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Toledano-Osorio, Manuel
Manzano-Moreno, Francisco J.
Toledano, Manuel
Medina-Castillo, Antonio L.
Costela-Ruiz, Victor J.
Ruiz, Concepción
Osorio, Raquel
Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
title Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
title_full Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
title_fullStr Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
title_full_unstemmed Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
title_short Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
title_sort doxycycline-doped polymeric membranes induced growth, differentiation and expression of antigenic phenotype markers of osteoblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037272/
https://www.ncbi.nlm.nih.gov/pubmed/33800569
http://dx.doi.org/10.3390/polym13071063
work_keys_str_mv AT toledanoosoriomanuel doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts
AT manzanomorenofranciscoj doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts
AT toledanomanuel doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts
AT medinacastilloantoniol doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts
AT costelaruizvictorj doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts
AT ruizconcepcion doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts
AT osorioraquel doxycyclinedopedpolymericmembranesinducedgrowthdifferentiationandexpressionofantigenicphenotypemarkersofosteoblasts