Cargando…
The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells
Despite intensive investigations, nanoparticle-induced cellular damage is an important problem that has not been fully elucidated yet. Here, we report that silica nanoparticles (SiNPs) demonstrated anticancer influence on glioblastoma cells by the induction of apoptosis or necrosis. These effects ar...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037515/ https://www.ncbi.nlm.nih.gov/pubmed/33808150 http://dx.doi.org/10.3390/ijms22073564 |
_version_ | 1783677161769533440 |
---|---|
author | Krętowski, Rafał Kusaczuk, Magdalena Naumowicz, Monika Cechowska-Pasko, Marzanna |
author_facet | Krętowski, Rafał Kusaczuk, Magdalena Naumowicz, Monika Cechowska-Pasko, Marzanna |
author_sort | Krętowski, Rafał |
collection | PubMed |
description | Despite intensive investigations, nanoparticle-induced cellular damage is an important problem that has not been fully elucidated yet. Here, we report that silica nanoparticles (SiNPs) demonstrated anticancer influence on glioblastoma cells by the induction of apoptosis or necrosis. These effects are highly cell type-specific, as well as dependent on the size and dose of applied nanoparticles. Exposure of LN-18 and LBC3 cells to different sizes of SiNPs—7 nm, 5–15 nm, or 10–20 nm—at dosages, ranging from 12.5 to 1000 µg/mL, for 24 and 48 h reduced the viability of these cells. Treatment of LN-18 and LBC3 cells with 7 nm or 10–20 nm SiNPs at doses ≥50 µg/mL caused a strong induction of apoptosis, which is connected with an increase of intracellular reactive oxygen species (ROS) production. The 5–15 nm SiNPs exhibited distinct behavior comparing to silica nanoparticles of other studied sizes. In contrast to LBC3, in LN-18 cells exposed to 5–15 nm SiNPs we did not observe any effect on apoptosis. These nanoparticles exerted only strong necrosis, which was connected with a reduction in ROS generation. This suggests that SiNPs can trigger different cellular/molecular effects, depending on the exposure conditions, the size and dose of nanoparticles, and cell type of glioblastoma. |
format | Online Article Text |
id | pubmed-8037515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80375152021-04-12 The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells Krętowski, Rafał Kusaczuk, Magdalena Naumowicz, Monika Cechowska-Pasko, Marzanna Int J Mol Sci Article Despite intensive investigations, nanoparticle-induced cellular damage is an important problem that has not been fully elucidated yet. Here, we report that silica nanoparticles (SiNPs) demonstrated anticancer influence on glioblastoma cells by the induction of apoptosis or necrosis. These effects are highly cell type-specific, as well as dependent on the size and dose of applied nanoparticles. Exposure of LN-18 and LBC3 cells to different sizes of SiNPs—7 nm, 5–15 nm, or 10–20 nm—at dosages, ranging from 12.5 to 1000 µg/mL, for 24 and 48 h reduced the viability of these cells. Treatment of LN-18 and LBC3 cells with 7 nm or 10–20 nm SiNPs at doses ≥50 µg/mL caused a strong induction of apoptosis, which is connected with an increase of intracellular reactive oxygen species (ROS) production. The 5–15 nm SiNPs exhibited distinct behavior comparing to silica nanoparticles of other studied sizes. In contrast to LBC3, in LN-18 cells exposed to 5–15 nm SiNPs we did not observe any effect on apoptosis. These nanoparticles exerted only strong necrosis, which was connected with a reduction in ROS generation. This suggests that SiNPs can trigger different cellular/molecular effects, depending on the exposure conditions, the size and dose of nanoparticles, and cell type of glioblastoma. MDPI 2021-03-30 /pmc/articles/PMC8037515/ /pubmed/33808150 http://dx.doi.org/10.3390/ijms22073564 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Krętowski, Rafał Kusaczuk, Magdalena Naumowicz, Monika Cechowska-Pasko, Marzanna The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells |
title | The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells |
title_full | The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells |
title_fullStr | The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells |
title_full_unstemmed | The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells |
title_short | The Pro-Apoptotic Effect of Silica Nanoparticles Depends on Their Size and Dose, as Well as the Type of Glioblastoma Cells |
title_sort | pro-apoptotic effect of silica nanoparticles depends on their size and dose, as well as the type of glioblastoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037515/ https://www.ncbi.nlm.nih.gov/pubmed/33808150 http://dx.doi.org/10.3390/ijms22073564 |
work_keys_str_mv | AT kretowskirafał theproapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT kusaczukmagdalena theproapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT naumowiczmonika theproapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT cechowskapaskomarzanna theproapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT kretowskirafał proapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT kusaczukmagdalena proapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT naumowiczmonika proapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells AT cechowskapaskomarzanna proapoptoticeffectofsilicananoparticlesdependsontheirsizeanddoseaswellasthetypeofglioblastomacells |