Cargando…
Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells
Korean Red ginseng (KRG), commonly used in traditional medicine, has anti-inflammatory, anti- oxidative, and anti-tumorigenic properties. Asian sand dust (ASD) is known to aggravate upper and lower airway inflammatory responses. BEAS-2B cells were exposed to ASD with or without KRG or ginsenoside Rg...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037637/ https://www.ncbi.nlm.nih.gov/pubmed/33916022 http://dx.doi.org/10.3390/molecules26072002 |
_version_ | 1783677190402998272 |
---|---|
author | Shin, Seung-Heon Ye, Mi-Kyung Lee, Dong-Won Kang, Byung-Jun Chae, Mi-Hyun |
author_facet | Shin, Seung-Heon Ye, Mi-Kyung Lee, Dong-Won Kang, Byung-Jun Chae, Mi-Hyun |
author_sort | Shin, Seung-Heon |
collection | PubMed |
description | Korean Red ginseng (KRG), commonly used in traditional medicine, has anti-inflammatory, anti- oxidative, and anti-tumorigenic properties. Asian sand dust (ASD) is known to aggravate upper and lower airway inflammatory responses. BEAS-2B cells were exposed to ASD with or without KRG or ginsenoside Rg3. Mucin 5AC (MUC5AC), MUC5B, and MUC8 mRNA and protein expression levels were determined using quantitative RT-PCR and enzyme-linked immunosorbent assay. Nuclear factor kappa B (NF-κB), activator protein 1, and mitogen-activated protein kinase expression and activity were determined using western blot analysis. ASD induced MUC5AC, MUC5B, and MUC8 mRNA and protein expression in BEAS-2B cells, which was significantly inhibited by KRG and Rg3. Although ASD-induced mucin expression was associated with NF-κB and p38 mitogen-activated protein kinase (MAPK) activity, KRG and Rg3 significantly suppressed only ASD-induced NF-κB expression and activity. KRG and Rg3 inhibited ASD-induced mucin gene expression and protein production from bronchial epithelial cells. These results suggest that KRG and Rg3 have potential for treating mucus-producing airway inflammatory diseases. |
format | Online Article Text |
id | pubmed-8037637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80376372021-04-12 Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells Shin, Seung-Heon Ye, Mi-Kyung Lee, Dong-Won Kang, Byung-Jun Chae, Mi-Hyun Molecules Article Korean Red ginseng (KRG), commonly used in traditional medicine, has anti-inflammatory, anti- oxidative, and anti-tumorigenic properties. Asian sand dust (ASD) is known to aggravate upper and lower airway inflammatory responses. BEAS-2B cells were exposed to ASD with or without KRG or ginsenoside Rg3. Mucin 5AC (MUC5AC), MUC5B, and MUC8 mRNA and protein expression levels were determined using quantitative RT-PCR and enzyme-linked immunosorbent assay. Nuclear factor kappa B (NF-κB), activator protein 1, and mitogen-activated protein kinase expression and activity were determined using western blot analysis. ASD induced MUC5AC, MUC5B, and MUC8 mRNA and protein expression in BEAS-2B cells, which was significantly inhibited by KRG and Rg3. Although ASD-induced mucin expression was associated with NF-κB and p38 mitogen-activated protein kinase (MAPK) activity, KRG and Rg3 significantly suppressed only ASD-induced NF-κB expression and activity. KRG and Rg3 inhibited ASD-induced mucin gene expression and protein production from bronchial epithelial cells. These results suggest that KRG and Rg3 have potential for treating mucus-producing airway inflammatory diseases. MDPI 2021-04-01 /pmc/articles/PMC8037637/ /pubmed/33916022 http://dx.doi.org/10.3390/molecules26072002 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shin, Seung-Heon Ye, Mi-Kyung Lee, Dong-Won Kang, Byung-Jun Chae, Mi-Hyun Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells |
title | Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells |
title_full | Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells |
title_fullStr | Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells |
title_full_unstemmed | Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells |
title_short | Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells |
title_sort | effect of korean red ginseng and rg3 on asian sand dust-induced muc5ac, muc5b, and muc8 expression in bronchial epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037637/ https://www.ncbi.nlm.nih.gov/pubmed/33916022 http://dx.doi.org/10.3390/molecules26072002 |
work_keys_str_mv | AT shinseungheon effectofkoreanredginsengandrg3onasiansanddustinducedmuc5acmuc5bandmuc8expressioninbronchialepithelialcells AT yemikyung effectofkoreanredginsengandrg3onasiansanddustinducedmuc5acmuc5bandmuc8expressioninbronchialepithelialcells AT leedongwon effectofkoreanredginsengandrg3onasiansanddustinducedmuc5acmuc5bandmuc8expressioninbronchialepithelialcells AT kangbyungjun effectofkoreanredginsengandrg3onasiansanddustinducedmuc5acmuc5bandmuc8expressioninbronchialepithelialcells AT chaemihyun effectofkoreanredginsengandrg3onasiansanddustinducedmuc5acmuc5bandmuc8expressioninbronchialepithelialcells |