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Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques

Porphyrinic compounds are widespread in nature and play key roles in biological processes such as oxygen transport in blood, enzymatic redox reactions or photosynthesis. In addition, both naturally derived as well as synthetic porphyrinic compounds are extensively explored for biomedical and technic...

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Autores principales: Gjuroski, Ilche, Furrer, Julien, Vermathen, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037866/
https://www.ncbi.nlm.nih.gov/pubmed/33808335
http://dx.doi.org/10.3390/molecules26071942
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author Gjuroski, Ilche
Furrer, Julien
Vermathen, Martina
author_facet Gjuroski, Ilche
Furrer, Julien
Vermathen, Martina
author_sort Gjuroski, Ilche
collection PubMed
description Porphyrinic compounds are widespread in nature and play key roles in biological processes such as oxygen transport in blood, enzymatic redox reactions or photosynthesis. In addition, both naturally derived as well as synthetic porphyrinic compounds are extensively explored for biomedical and technical applications such as photodynamic therapy (PDT) or photovoltaic systems, respectively. Their unique electronic structures and photophysical properties make this class of compounds so interesting for the multiple functions encountered. It is therefore not surprising that optical methods are typically the prevalent analytical tool applied in characterization and processes involving porphyrinic compounds. However, a wealth of complementary information can be obtained from NMR spectroscopic techniques. Based on the advantage of providing structural and dynamic information with atomic resolution simultaneously, NMR spectroscopy is a powerful method for studying molecular interactions between porphyrinic compounds and macromolecules. Such interactions are of special interest in medical applications of porphyrinic photosensitizers that are mostly combined with macromolecular carrier systems. The macromolecular surrounding typically stabilizes the encapsulated drug and may also modify its physical properties. Moreover, the interaction with macromolecular physiological components needs to be explored to understand and control mechanisms of action and therapeutic efficacy. This review focuses on such non-covalent interactions of porphyrinic drugs with synthetic polymers as well as with biomolecules such as phospholipids or proteins. A brief introduction into various NMR spectroscopic techniques is given including chemical shift perturbation methods, NOE enhancement spectroscopy, relaxation time measurements and diffusion-ordered spectroscopy. How these NMR tools are used to address porphyrin–macromolecule interactions with respect to their function in biomedical applications is the central point of the current review.
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spelling pubmed-80378662021-04-12 Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques Gjuroski, Ilche Furrer, Julien Vermathen, Martina Molecules Review Porphyrinic compounds are widespread in nature and play key roles in biological processes such as oxygen transport in blood, enzymatic redox reactions or photosynthesis. In addition, both naturally derived as well as synthetic porphyrinic compounds are extensively explored for biomedical and technical applications such as photodynamic therapy (PDT) or photovoltaic systems, respectively. Their unique electronic structures and photophysical properties make this class of compounds so interesting for the multiple functions encountered. It is therefore not surprising that optical methods are typically the prevalent analytical tool applied in characterization and processes involving porphyrinic compounds. However, a wealth of complementary information can be obtained from NMR spectroscopic techniques. Based on the advantage of providing structural and dynamic information with atomic resolution simultaneously, NMR spectroscopy is a powerful method for studying molecular interactions between porphyrinic compounds and macromolecules. Such interactions are of special interest in medical applications of porphyrinic photosensitizers that are mostly combined with macromolecular carrier systems. The macromolecular surrounding typically stabilizes the encapsulated drug and may also modify its physical properties. Moreover, the interaction with macromolecular physiological components needs to be explored to understand and control mechanisms of action and therapeutic efficacy. This review focuses on such non-covalent interactions of porphyrinic drugs with synthetic polymers as well as with biomolecules such as phospholipids or proteins. A brief introduction into various NMR spectroscopic techniques is given including chemical shift perturbation methods, NOE enhancement spectroscopy, relaxation time measurements and diffusion-ordered spectroscopy. How these NMR tools are used to address porphyrin–macromolecule interactions with respect to their function in biomedical applications is the central point of the current review. MDPI 2021-03-30 /pmc/articles/PMC8037866/ /pubmed/33808335 http://dx.doi.org/10.3390/molecules26071942 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gjuroski, Ilche
Furrer, Julien
Vermathen, Martina
Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
title Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
title_full Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
title_fullStr Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
title_full_unstemmed Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
title_short Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
title_sort probing the interactions of porphyrins with macromolecules using nmr spectroscopy techniques
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037866/
https://www.ncbi.nlm.nih.gov/pubmed/33808335
http://dx.doi.org/10.3390/molecules26071942
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