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Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium

The deuterium content modification in an organism has a neuroprotective effect during the hypoxia model, affecting anxiety, memory and stress resistance. The aim of this work was to elucidate the possible mechanisms of the medium D/H composition modification on nerve cells. We studied the effect of...

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Autores principales: Kozin, Stanislav, Skrebitsky, Vladimir, Kondratenko, Rodion, Kravtsov, Alexander, Butina, Elena, Moiseev, Arkady, Malyshko, Vadim, Baryshev, Mikhail, Elkina, Anna, Dzhimak, Stepan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038400/
https://www.ncbi.nlm.nih.gov/pubmed/33918525
http://dx.doi.org/10.3390/molecules26072036
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author Kozin, Stanislav
Skrebitsky, Vladimir
Kondratenko, Rodion
Kravtsov, Alexander
Butina, Elena
Moiseev, Arkady
Malyshko, Vadim
Baryshev, Mikhail
Elkina, Anna
Dzhimak, Stepan
author_facet Kozin, Stanislav
Skrebitsky, Vladimir
Kondratenko, Rodion
Kravtsov, Alexander
Butina, Elena
Moiseev, Arkady
Malyshko, Vadim
Baryshev, Mikhail
Elkina, Anna
Dzhimak, Stepan
author_sort Kozin, Stanislav
collection PubMed
description The deuterium content modification in an organism has a neuroprotective effect during the hypoxia model, affecting anxiety, memory and stress resistance. The aim of this work was to elucidate the possible mechanisms of the medium D/H composition modification on nerve cells. We studied the effect of an incubation medium with a 50 ppm deuterium content compared to a medium with 150 ppm on: (1) the activity of Wistar rats’ hippocampus CA1 field neurons, (2) the level of cultured cerebellar neuron death during glucose deprivation and temperature stress, (3) mitochondrial membrane potential (MMP) and the generation of reactive oxygen species in cultures of cerebellar neurons. The results of the analysis showed that the incubation of hippocampal sections in a medium with a 50 ppm deuterium reduced the amplitude of the pop-spike. The restoration of neuron activity was observed when sections were returned to the incubation medium with a 150 ppm deuterium content. An environment with a 50 ppm deuterium did not significantly affect the level of reactive oxygen species in neuron cultures, while MMP decreased by 16–20%. In experiments with glucose deprivation and temperature stress, the medium with 50 ppm increased the death of neurons. Thus, a short exposure of nerve cells in the medium with 50 ppm deuterium acts as an additional stressful factor, which is possibly associated with the violation of the cell energy balance. The decrease in the mitochondrial membrane potential, which is known to be associated with ATP synthesis, indicates that this effect may be associated with the cell energy imbalance. The decrease in the activity of the CA1 field hippocampal neurons may reflect reversible adaptive changes in the operation of fast-reacting ion channels.
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spelling pubmed-80384002021-04-12 Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium Kozin, Stanislav Skrebitsky, Vladimir Kondratenko, Rodion Kravtsov, Alexander Butina, Elena Moiseev, Arkady Malyshko, Vadim Baryshev, Mikhail Elkina, Anna Dzhimak, Stepan Molecules Article The deuterium content modification in an organism has a neuroprotective effect during the hypoxia model, affecting anxiety, memory and stress resistance. The aim of this work was to elucidate the possible mechanisms of the medium D/H composition modification on nerve cells. We studied the effect of an incubation medium with a 50 ppm deuterium content compared to a medium with 150 ppm on: (1) the activity of Wistar rats’ hippocampus CA1 field neurons, (2) the level of cultured cerebellar neuron death during glucose deprivation and temperature stress, (3) mitochondrial membrane potential (MMP) and the generation of reactive oxygen species in cultures of cerebellar neurons. The results of the analysis showed that the incubation of hippocampal sections in a medium with a 50 ppm deuterium reduced the amplitude of the pop-spike. The restoration of neuron activity was observed when sections were returned to the incubation medium with a 150 ppm deuterium content. An environment with a 50 ppm deuterium did not significantly affect the level of reactive oxygen species in neuron cultures, while MMP decreased by 16–20%. In experiments with glucose deprivation and temperature stress, the medium with 50 ppm increased the death of neurons. Thus, a short exposure of nerve cells in the medium with 50 ppm deuterium acts as an additional stressful factor, which is possibly associated with the violation of the cell energy balance. The decrease in the mitochondrial membrane potential, which is known to be associated with ATP synthesis, indicates that this effect may be associated with the cell energy imbalance. The decrease in the activity of the CA1 field hippocampal neurons may reflect reversible adaptive changes in the operation of fast-reacting ion channels. MDPI 2021-04-02 /pmc/articles/PMC8038400/ /pubmed/33918525 http://dx.doi.org/10.3390/molecules26072036 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kozin, Stanislav
Skrebitsky, Vladimir
Kondratenko, Rodion
Kravtsov, Alexander
Butina, Elena
Moiseev, Arkady
Malyshko, Vadim
Baryshev, Mikhail
Elkina, Anna
Dzhimak, Stepan
Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium
title Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium
title_full Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium
title_fullStr Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium
title_full_unstemmed Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium
title_short Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium
title_sort electrophysiological activity and survival rate of rats nervous tissue cells depends on d/h isotopic composition of medium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038400/
https://www.ncbi.nlm.nih.gov/pubmed/33918525
http://dx.doi.org/10.3390/molecules26072036
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