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Base editing: a brief review and a practical example

Genome editing has undergone rapid development in recent years, yielding new approaches to make precise changes in genes. In this review, we discuss the development of various adenine and cytosine base-editing technologies, which share the ability to make specific base changes at specific sites in t...

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Detalles Bibliográficos
Autores principales: Choe, Dongwook C., Musunuru, Kiran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038524/
https://www.ncbi.nlm.nih.gov/pubmed/32934192
http://dx.doi.org/10.7555/JBR.34.20200003
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author Choe, Dongwook C.
Musunuru, Kiran
author_facet Choe, Dongwook C.
Musunuru, Kiran
author_sort Choe, Dongwook C.
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description Genome editing has undergone rapid development in recent years, yielding new approaches to make precise changes in genes. In this review, we discuss the development of various adenine and cytosine base-editing technologies, which share the ability to make specific base changes at specific sites in the genome. We also describe multiple applications of base editing in vitro and in vivo. Finally, as a practical example, we demonstrate the use of a cytosine base editor and an adenine base editor in human cells to introduce and then correct a prevalent mutation responsible for hereditary tyrosinemia type 1.
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spelling pubmed-80385242021-04-16 Base editing: a brief review and a practical example Choe, Dongwook C. Musunuru, Kiran J Biomed Res Review Article Genome editing has undergone rapid development in recent years, yielding new approaches to make precise changes in genes. In this review, we discuss the development of various adenine and cytosine base-editing technologies, which share the ability to make specific base changes at specific sites in the genome. We also describe multiple applications of base editing in vitro and in vivo. Finally, as a practical example, we demonstrate the use of a cytosine base editor and an adenine base editor in human cells to introduce and then correct a prevalent mutation responsible for hereditary tyrosinemia type 1. Editorial Department of Journal of Biomedical Research 2021-03 2020-07-31 /pmc/articles/PMC8038524/ /pubmed/32934192 http://dx.doi.org/10.7555/JBR.34.20200003 Text en Copyright and License information: Journal of Biomedical Research, CAS Springer-Verlag Berlin Heidelberg 2021 https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/)
spellingShingle Review Article
Choe, Dongwook C.
Musunuru, Kiran
Base editing: a brief review and a practical example
title Base editing: a brief review and a practical example
title_full Base editing: a brief review and a practical example
title_fullStr Base editing: a brief review and a practical example
title_full_unstemmed Base editing: a brief review and a practical example
title_short Base editing: a brief review and a practical example
title_sort base editing: a brief review and a practical example
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038524/
https://www.ncbi.nlm.nih.gov/pubmed/32934192
http://dx.doi.org/10.7555/JBR.34.20200003
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