QuEChERS LC–MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices

We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of...

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Detalles Bibliográficos
Autores principales: Pantano, Licia, La Scala, Ladislao, Olibrio, Francesco, Galluzzo, Francesco Giuseppe, Bongiorno, Carmelo, Buscemi, Maria Drussilla, Macaluso, Andrea, Vella, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038554/
https://www.ncbi.nlm.nih.gov/pubmed/33916634
http://dx.doi.org/10.3390/ijerph18073774
Descripción
Sumario:We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB(1), AFB(2), AFG(1), AFG(2)), deoxynivalenol (DON), fumonisin (FUMO; FB(1), FB(2), FB(3)), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method’s performance was assessed by two different proficiencies and tested with 100 real samples.

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