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Key wheat GRF genes constraining wheat tillering of mutant dmc
Tillering is a key agronomy trait for wheat (Triticum aestivum L.) production. Previously, we have reported a dwarf-monoculm wheat mutant (dmc) obtained from cultivar Guomai 301 (wild type, WT), and found growth regulating factors (GRFs) playing important roles in regulating wheat tillering. This st...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038642/ https://www.ncbi.nlm.nih.gov/pubmed/33889451 http://dx.doi.org/10.7717/peerj.11235 |
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author | Zhang, Jing Li, Junchang Ni, Yongjing Jiang, Yumei Jiao, Zhixin Li, Huijuan Wang, Ting Zhang, Peipei Han, Mengyao Li, Lei Liu, Hongjie Li, Qiaoyun Niu, Jishan |
author_facet | Zhang, Jing Li, Junchang Ni, Yongjing Jiang, Yumei Jiao, Zhixin Li, Huijuan Wang, Ting Zhang, Peipei Han, Mengyao Li, Lei Liu, Hongjie Li, Qiaoyun Niu, Jishan |
author_sort | Zhang, Jing |
collection | PubMed |
description | Tillering is a key agronomy trait for wheat (Triticum aestivum L.) production. Previously, we have reported a dwarf-monoculm wheat mutant (dmc) obtained from cultivar Guomai 301 (wild type, WT), and found growth regulating factors (GRFs) playing important roles in regulating wheat tillering. This study is to systematically investigate the roles of all the wheat GRFs (T. aestivum GRFs, TaGRFs) in regulating tillering, and screen out the key regulators. A total of 30 TaGRFs were identified and their physicochemical properties, gene structures, conserved domains, phylogenetic relationships and tissue expression profiles were analyzed. The expression levels of all the TaGRFs were significantly lower in dmc than those in WT at early tillering stage, and the abnormal expressions of TaGRF2-7(A, B, D), TaGRF5-7D, TaGRF10-6(A, B, D) and TaGRF11-2A were major causes constraining the tillering of dmc. The transcriptions of TaGRFs were significantly affected by exogenous indole acetic acid (IAA) and gibberellin acid (GA3) applications, which suggested that TaGRFs as well as IAA, GA signaling were involved in controlling wheat tillering. This study provided valuable clues for functional characterization of GRF genes in wheat. |
format | Online Article Text |
id | pubmed-8038642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80386422021-04-21 Key wheat GRF genes constraining wheat tillering of mutant dmc Zhang, Jing Li, Junchang Ni, Yongjing Jiang, Yumei Jiao, Zhixin Li, Huijuan Wang, Ting Zhang, Peipei Han, Mengyao Li, Lei Liu, Hongjie Li, Qiaoyun Niu, Jishan PeerJ Agricultural Science Tillering is a key agronomy trait for wheat (Triticum aestivum L.) production. Previously, we have reported a dwarf-monoculm wheat mutant (dmc) obtained from cultivar Guomai 301 (wild type, WT), and found growth regulating factors (GRFs) playing important roles in regulating wheat tillering. This study is to systematically investigate the roles of all the wheat GRFs (T. aestivum GRFs, TaGRFs) in regulating tillering, and screen out the key regulators. A total of 30 TaGRFs were identified and their physicochemical properties, gene structures, conserved domains, phylogenetic relationships and tissue expression profiles were analyzed. The expression levels of all the TaGRFs were significantly lower in dmc than those in WT at early tillering stage, and the abnormal expressions of TaGRF2-7(A, B, D), TaGRF5-7D, TaGRF10-6(A, B, D) and TaGRF11-2A were major causes constraining the tillering of dmc. The transcriptions of TaGRFs were significantly affected by exogenous indole acetic acid (IAA) and gibberellin acid (GA3) applications, which suggested that TaGRFs as well as IAA, GA signaling were involved in controlling wheat tillering. This study provided valuable clues for functional characterization of GRF genes in wheat. PeerJ Inc. 2021-04-08 /pmc/articles/PMC8038642/ /pubmed/33889451 http://dx.doi.org/10.7717/peerj.11235 Text en © 2021 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Zhang, Jing Li, Junchang Ni, Yongjing Jiang, Yumei Jiao, Zhixin Li, Huijuan Wang, Ting Zhang, Peipei Han, Mengyao Li, Lei Liu, Hongjie Li, Qiaoyun Niu, Jishan Key wheat GRF genes constraining wheat tillering of mutant dmc |
title | Key wheat GRF genes constraining wheat tillering of mutant dmc |
title_full | Key wheat GRF genes constraining wheat tillering of mutant dmc |
title_fullStr | Key wheat GRF genes constraining wheat tillering of mutant dmc |
title_full_unstemmed | Key wheat GRF genes constraining wheat tillering of mutant dmc |
title_short | Key wheat GRF genes constraining wheat tillering of mutant dmc |
title_sort | key wheat grf genes constraining wheat tillering of mutant dmc |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038642/ https://www.ncbi.nlm.nih.gov/pubmed/33889451 http://dx.doi.org/10.7717/peerj.11235 |
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