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Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage

Alterations in the metabolism of sphingolipids, a class of biologically active molecules in cell membranes with direct effect on vascular homeostasis, are increasingly recognized as important determinant in different vascular disorders. However, it is not clear whether sphingolipids are implicated i...

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Autores principales: Pepe, Giuseppe, Cotugno, Maria, Marracino, Federico, Giova, Susy, Capocci, Luca, Forte, Maurizio, Stanzione, Rosita, Bianchi, Franca, Marchitti, Simona, Di Pardo, Alba, Sciarretta, Sebastiano, Rubattu, Speranza, Maglione, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038804/
https://www.ncbi.nlm.nih.gov/pubmed/33917593
http://dx.doi.org/10.3390/ijms22073796
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author Pepe, Giuseppe
Cotugno, Maria
Marracino, Federico
Giova, Susy
Capocci, Luca
Forte, Maurizio
Stanzione, Rosita
Bianchi, Franca
Marchitti, Simona
Di Pardo, Alba
Sciarretta, Sebastiano
Rubattu, Speranza
Maglione, Vittorio
author_facet Pepe, Giuseppe
Cotugno, Maria
Marracino, Federico
Giova, Susy
Capocci, Luca
Forte, Maurizio
Stanzione, Rosita
Bianchi, Franca
Marchitti, Simona
Di Pardo, Alba
Sciarretta, Sebastiano
Rubattu, Speranza
Maglione, Vittorio
author_sort Pepe, Giuseppe
collection PubMed
description Alterations in the metabolism of sphingolipids, a class of biologically active molecules in cell membranes with direct effect on vascular homeostasis, are increasingly recognized as important determinant in different vascular disorders. However, it is not clear whether sphingolipids are implicated in the pathogenesis of hypertension-related cerebrovascular and renal damage. In this study, we evaluated the existence of possible abnormalities related to the sphingolipid metabolism in the brain and kidneys of two well validated spontaneously hypertensive rat strains, the stroke-prone (SHRSP) and the stroke-resistant (SHRSR) models, as compared to the normotensive Wistar Kyoto (WKY) rat strain. Our results showed a global alteration in the metabolism of sphingolipids in both cerebral and renal tissues of both hypertensive strains as compared to the normotensive rat. However, few defects, such as reduced expression of enzymes involved in the metabolism/catabolism of sphingosine-1-phosphate and in the de novo biosynthetic pathways, were exclusively detected in the SHRSP. Although further studies are necessary to fully understand the significance of these findings, they suggest that defects in specific lipid molecules and/or their related metabolic pathways may likely contribute to the pathogenesis of hypertensive target organ damage and may eventually serve as future therapeutic targets to reduce the vascular consequences of hypertension.
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spelling pubmed-80388042021-04-12 Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage Pepe, Giuseppe Cotugno, Maria Marracino, Federico Giova, Susy Capocci, Luca Forte, Maurizio Stanzione, Rosita Bianchi, Franca Marchitti, Simona Di Pardo, Alba Sciarretta, Sebastiano Rubattu, Speranza Maglione, Vittorio Int J Mol Sci Article Alterations in the metabolism of sphingolipids, a class of biologically active molecules in cell membranes with direct effect on vascular homeostasis, are increasingly recognized as important determinant in different vascular disorders. However, it is not clear whether sphingolipids are implicated in the pathogenesis of hypertension-related cerebrovascular and renal damage. In this study, we evaluated the existence of possible abnormalities related to the sphingolipid metabolism in the brain and kidneys of two well validated spontaneously hypertensive rat strains, the stroke-prone (SHRSP) and the stroke-resistant (SHRSR) models, as compared to the normotensive Wistar Kyoto (WKY) rat strain. Our results showed a global alteration in the metabolism of sphingolipids in both cerebral and renal tissues of both hypertensive strains as compared to the normotensive rat. However, few defects, such as reduced expression of enzymes involved in the metabolism/catabolism of sphingosine-1-phosphate and in the de novo biosynthetic pathways, were exclusively detected in the SHRSP. Although further studies are necessary to fully understand the significance of these findings, they suggest that defects in specific lipid molecules and/or their related metabolic pathways may likely contribute to the pathogenesis of hypertensive target organ damage and may eventually serve as future therapeutic targets to reduce the vascular consequences of hypertension. MDPI 2021-04-06 /pmc/articles/PMC8038804/ /pubmed/33917593 http://dx.doi.org/10.3390/ijms22073796 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pepe, Giuseppe
Cotugno, Maria
Marracino, Federico
Giova, Susy
Capocci, Luca
Forte, Maurizio
Stanzione, Rosita
Bianchi, Franca
Marchitti, Simona
Di Pardo, Alba
Sciarretta, Sebastiano
Rubattu, Speranza
Maglione, Vittorio
Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
title Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
title_full Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
title_fullStr Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
title_full_unstemmed Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
title_short Differential Expression of Sphingolipid Metabolizing Enzymes in Spontaneously Hypertensive Rats: A Possible Substrate for Susceptibility to Brain and Kidney Damage
title_sort differential expression of sphingolipid metabolizing enzymes in spontaneously hypertensive rats: a possible substrate for susceptibility to brain and kidney damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038804/
https://www.ncbi.nlm.nih.gov/pubmed/33917593
http://dx.doi.org/10.3390/ijms22073796
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