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Parasite Burden Measurement in the Leishmania major Infected Mice by Using the Direct Fluorescent Microscopy, Limiting Dilution Assay, and Real-Time PCR Analysis

BACKGROUND: We aimed to compare parasite burden in BALB/c mice, using three methods including the direct fluorescent microscopic using recombinant Leishmania major expressing an enhanced green fluorescent protein, limiting dilution assay, and real-time PCR technique. METHODS: The current study was c...

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Detalles Bibliográficos
Autores principales: HAGHDOUST, Sepideh, AZIZI, Mahdieh, HAJI MOLLA HOSEINI, Mostafa, BANDEHPOUR, Mojgan, MOHSENI MASOOLEH, Mandana, YEGANEH, Farshid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039490/
https://www.ncbi.nlm.nih.gov/pubmed/33884015
http://dx.doi.org/10.18502/ijpa.v15i4.4867