Cargando…
Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis
BACKGROUND: Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by Toxocara canis. We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers. METHODS: This study was conducted on 324 individu...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039491/ https://www.ncbi.nlm.nih.gov/pubmed/33884013 http://dx.doi.org/10.18502/ijpa.v15i4.4865 |
_version_ | 1783677602534260736 |
---|---|
author | RAISSI, Vahid MOHEBALI, Mehdi KIA, Eshrat Bigom RAHIMI FOROUSHANI, Abbas SOHRABI, Nasrin ROKNI, Mohammad Bagher ZIBAEI, Mohammad |
author_facet | RAISSI, Vahid MOHEBALI, Mehdi KIA, Eshrat Bigom RAHIMI FOROUSHANI, Abbas SOHRABI, Nasrin ROKNI, Mohammad Bagher ZIBAEI, Mohammad |
author_sort | RAISSI, Vahid |
collection | PubMed |
description | BACKGROUND: Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by Toxocara canis. We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers. METHODS: This study was conducted on 324 individuals for ELISA test on toxocariasis in Tehran and Karaj, Iran 2019. Then positive samples for anti-Toxocara IgG were obtained to quantitative Real-time PCR (qRT-PCR) assays to investigate the transcriptional profiles of miRNAs predicted to be involved in developmental and reproductive processes. qPCR was employed to assess levels of transcription for miRNAs of 103a and 21 in plasma samples. RESULTS: After the experiments, the results were evaluated by REST software, Livak formula and quantitative t-test. The analyzes performed on human samples showed that in the case group compared to the control group, only in Tc-miR-21 gene, a 0.3-fold increase in expression was obtained with REST software (Fold change ≤ 1.5, P>0.05), which was statistically significant by t-test (P<0.05). CONCLUSION: To our knowledge, this is the first study to evaluate miR-21 and miR-103a in toxocariasis, which shed light on the fundamental role of it as a biomarker and diagnostic tool. However, due to the changes in expression of these miRNAs were not vast to be used as biomarkers in diagnosis. Despite of that the changes in the expression of these miRNAs were not vast but they could serve as novel promising biomarkers for diagnosis of toxocariasis. |
format | Online Article Text |
id | pubmed-8039491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-80394912021-04-20 Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis RAISSI, Vahid MOHEBALI, Mehdi KIA, Eshrat Bigom RAHIMI FOROUSHANI, Abbas SOHRABI, Nasrin ROKNI, Mohammad Bagher ZIBAEI, Mohammad Iran J Parasitol Original Article BACKGROUND: Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by Toxocara canis. We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers. METHODS: This study was conducted on 324 individuals for ELISA test on toxocariasis in Tehran and Karaj, Iran 2019. Then positive samples for anti-Toxocara IgG were obtained to quantitative Real-time PCR (qRT-PCR) assays to investigate the transcriptional profiles of miRNAs predicted to be involved in developmental and reproductive processes. qPCR was employed to assess levels of transcription for miRNAs of 103a and 21 in plasma samples. RESULTS: After the experiments, the results were evaluated by REST software, Livak formula and quantitative t-test. The analyzes performed on human samples showed that in the case group compared to the control group, only in Tc-miR-21 gene, a 0.3-fold increase in expression was obtained with REST software (Fold change ≤ 1.5, P>0.05), which was statistically significant by t-test (P<0.05). CONCLUSION: To our knowledge, this is the first study to evaluate miR-21 and miR-103a in toxocariasis, which shed light on the fundamental role of it as a biomarker and diagnostic tool. However, due to the changes in expression of these miRNAs were not vast to be used as biomarkers in diagnosis. Despite of that the changes in the expression of these miRNAs were not vast but they could serve as novel promising biomarkers for diagnosis of toxocariasis. Tehran University of Medical Sciences 2020 /pmc/articles/PMC8039491/ /pubmed/33884013 http://dx.doi.org/10.18502/ijpa.v15i4.4865 Text en Copyright © 2020 Raissi et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article RAISSI, Vahid MOHEBALI, Mehdi KIA, Eshrat Bigom RAHIMI FOROUSHANI, Abbas SOHRABI, Nasrin ROKNI, Mohammad Bagher ZIBAEI, Mohammad Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis |
title | Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis |
title_full | Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis |
title_fullStr | Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis |
title_full_unstemmed | Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis |
title_short | Expression of Mir-21 and Mir-103a in Toxocara canis: Potential for Diagnosis of Human Toxocariasis |
title_sort | expression of mir-21 and mir-103a in toxocara canis: potential for diagnosis of human toxocariasis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039491/ https://www.ncbi.nlm.nih.gov/pubmed/33884013 http://dx.doi.org/10.18502/ijpa.v15i4.4865 |
work_keys_str_mv | AT raissivahid expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis AT mohebalimehdi expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis AT kiaeshratbigom expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis AT rahimiforoushaniabbas expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis AT sohrabinasrin expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis AT roknimohammadbagher expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis AT zibaeimohammad expressionofmir21andmir103aintoxocaracanispotentialfordiagnosisofhumantoxocariasis |