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Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039529/ https://www.ncbi.nlm.nih.gov/pubmed/33855024 http://dx.doi.org/10.3389/fcell.2021.647892 |
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author | Liu, Xiaoli Li, Wei Yang, Yanping Chen, Kaili Li, Yulin Zhu, Xinping Ye, Hua Xu, Hongyan |
author_facet | Liu, Xiaoli Li, Wei Yang, Yanping Chen, Kaili Li, Yulin Zhu, Xinping Ye, Hua Xu, Hongyan |
author_sort | Liu, Xiaoli |
collection | PubMed |
description | Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals; however, there is limited scRNA-seq analysis on gonadal cells in lower vertebrates, especially in the sexual reversal fish species. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq. We identified five cell types and also successfully validated some specific genes of germ cells and granulosa cells. Likewise, we found some key pathways involved in ovarian development that may concert germline-somatic interactions. Moreover, we compared the transcriptomic profiles across fruit fly, mammals, and fish, and thus uncovered the conservation and divergence in molecular mechanisms that might drive ovarian development. Our results provide a basis for studying the crucial features of germ cells and somatic cells, which will benefit the understandings of the molecular mechanisms behind gametogenesis and gonad development in fish. |
format | Online Article Text |
id | pubmed-8039529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80395292021-04-13 Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass Liu, Xiaoli Li, Wei Yang, Yanping Chen, Kaili Li, Yulin Zhu, Xinping Ye, Hua Xu, Hongyan Front Cell Dev Biol Cell and Developmental Biology Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals; however, there is limited scRNA-seq analysis on gonadal cells in lower vertebrates, especially in the sexual reversal fish species. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq. We identified five cell types and also successfully validated some specific genes of germ cells and granulosa cells. Likewise, we found some key pathways involved in ovarian development that may concert germline-somatic interactions. Moreover, we compared the transcriptomic profiles across fruit fly, mammals, and fish, and thus uncovered the conservation and divergence in molecular mechanisms that might drive ovarian development. Our results provide a basis for studying the crucial features of germ cells and somatic cells, which will benefit the understandings of the molecular mechanisms behind gametogenesis and gonad development in fish. Frontiers Media S.A. 2021-03-29 /pmc/articles/PMC8039529/ /pubmed/33855024 http://dx.doi.org/10.3389/fcell.2021.647892 Text en Copyright © 2021 Liu, Li, Yang, Chen, Li, Zhu, Ye and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Liu, Xiaoli Li, Wei Yang, Yanping Chen, Kaili Li, Yulin Zhu, Xinping Ye, Hua Xu, Hongyan Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
title | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
title_full | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
title_fullStr | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
title_full_unstemmed | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
title_short | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
title_sort | transcriptome profiling of the ovarian cells at the single-cell resolution in adult asian seabass |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039529/ https://www.ncbi.nlm.nih.gov/pubmed/33855024 http://dx.doi.org/10.3389/fcell.2021.647892 |
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