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Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass

Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate t...

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Autores principales: Liu, Xiaoli, Li, Wei, Yang, Yanping, Chen, Kaili, Li, Yulin, Zhu, Xinping, Ye, Hua, Xu, Hongyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039529/
https://www.ncbi.nlm.nih.gov/pubmed/33855024
http://dx.doi.org/10.3389/fcell.2021.647892
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author Liu, Xiaoli
Li, Wei
Yang, Yanping
Chen, Kaili
Li, Yulin
Zhu, Xinping
Ye, Hua
Xu, Hongyan
author_facet Liu, Xiaoli
Li, Wei
Yang, Yanping
Chen, Kaili
Li, Yulin
Zhu, Xinping
Ye, Hua
Xu, Hongyan
author_sort Liu, Xiaoli
collection PubMed
description Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals; however, there is limited scRNA-seq analysis on gonadal cells in lower vertebrates, especially in the sexual reversal fish species. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq. We identified five cell types and also successfully validated some specific genes of germ cells and granulosa cells. Likewise, we found some key pathways involved in ovarian development that may concert germline-somatic interactions. Moreover, we compared the transcriptomic profiles across fruit fly, mammals, and fish, and thus uncovered the conservation and divergence in molecular mechanisms that might drive ovarian development. Our results provide a basis for studying the crucial features of germ cells and somatic cells, which will benefit the understandings of the molecular mechanisms behind gametogenesis and gonad development in fish.
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spelling pubmed-80395292021-04-13 Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass Liu, Xiaoli Li, Wei Yang, Yanping Chen, Kaili Li, Yulin Zhu, Xinping Ye, Hua Xu, Hongyan Front Cell Dev Biol Cell and Developmental Biology Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals; however, there is limited scRNA-seq analysis on gonadal cells in lower vertebrates, especially in the sexual reversal fish species. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq. We identified five cell types and also successfully validated some specific genes of germ cells and granulosa cells. Likewise, we found some key pathways involved in ovarian development that may concert germline-somatic interactions. Moreover, we compared the transcriptomic profiles across fruit fly, mammals, and fish, and thus uncovered the conservation and divergence in molecular mechanisms that might drive ovarian development. Our results provide a basis for studying the crucial features of germ cells and somatic cells, which will benefit the understandings of the molecular mechanisms behind gametogenesis and gonad development in fish. Frontiers Media S.A. 2021-03-29 /pmc/articles/PMC8039529/ /pubmed/33855024 http://dx.doi.org/10.3389/fcell.2021.647892 Text en Copyright © 2021 Liu, Li, Yang, Chen, Li, Zhu, Ye and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Liu, Xiaoli
Li, Wei
Yang, Yanping
Chen, Kaili
Li, Yulin
Zhu, Xinping
Ye, Hua
Xu, Hongyan
Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
title Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
title_full Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
title_fullStr Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
title_full_unstemmed Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
title_short Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
title_sort transcriptome profiling of the ovarian cells at the single-cell resolution in adult asian seabass
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039529/
https://www.ncbi.nlm.nih.gov/pubmed/33855024
http://dx.doi.org/10.3389/fcell.2021.647892
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