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Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer
BACKGROUND: The BC-6000Plus (Mindray, Shenzhen, China) is a recently developed hematology analyzer that utilizes fluorescent technology. Based on fluorescent nucleic acid stain and optical detection, the optical platelet counting (PLT-O) on the BC-6000Plus has strong anti-interference potential in p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039664/ https://www.ncbi.nlm.nih.gov/pubmed/33850905 http://dx.doi.org/10.21037/atm-21-691 |
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author | Hu, Xuehua Tang, Yuqun Li, Mingyi Fu, Chunyun Deng, Zuoxin Tang, Wenting Zhou, Huideng Chen, Yan Li, Yingfeng |
author_facet | Hu, Xuehua Tang, Yuqun Li, Mingyi Fu, Chunyun Deng, Zuoxin Tang, Wenting Zhou, Huideng Chen, Yan Li, Yingfeng |
author_sort | Hu, Xuehua |
collection | PubMed |
description | BACKGROUND: The BC-6000Plus (Mindray, Shenzhen, China) is a recently developed hematology analyzer that utilizes fluorescent technology. Based on fluorescent nucleic acid stain and optical detection, the optical platelet counting (PLT-O) on the BC-6000Plus has strong anti-interference potential in platelet (PLT) detection. Its Auto 8×PLT-O Counting Tech can be automatically triggered in low-PLT samples, which enables the PLT-O on the BC-6000Plus to count low PLT more efficiently. Here, we evaluated the performance of the BC-6000Plus automated hematology analyzer in optical PLT counting. METHODS: The basic features (including blank counting, carryover, trueness, and accuracy) of the BC-6000Plus for PLT counting were evaluated according to the Analytical Quality Specifications for Routine Tests in Clinical Hematology (WST 406-2012). Low-PLT samples with a PLT count of below 100×10(9)/L were selected for repeatability tests. Meanwhile, the potential correlations of BC-6000Plus with the XN-L 350 and manual microscopy within different PLT ranges or under interferences of small red blood cells (RBCs) or PLT aggregation were analyzed. RESULTS: The PLT-O on BC-6000Plus met the technical requirements of PLT counting in terms of blank count, carryover, trueness, and accuracy. The repeatability of the enhanced mode (PLT-O 8×) on the BC-6000Plus was better than that of the XN-L 350 in three low PLT count ranges, including 10–20, 20–60, and 60–100 (×10(9)/L). Under the interference-free conditions, the BC-6000Plus correlated well with the XN-L 350 in different PLT counting ranges. Under the interferences of small RBCs and PLT aggregation, the PLT-O on BC-6000Plus correlated better with microscopy than with the platelet impedance count (PLT-I). CONCLUSIONS: The PLT-O on BC-6000Plus can meet the technical requirements of PLT counting in terms of blank counting, carryover rate, trueness, and accuracy. The PLT-O 8× has good repeatability, correlates well with the XN-L 350, and demonstrates good anti-interference ability. It can thus meet the needs of blood cell analysis in clinical settings. |
format | Online Article Text |
id | pubmed-8039664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-80396642021-04-12 Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer Hu, Xuehua Tang, Yuqun Li, Mingyi Fu, Chunyun Deng, Zuoxin Tang, Wenting Zhou, Huideng Chen, Yan Li, Yingfeng Ann Transl Med Original Article BACKGROUND: The BC-6000Plus (Mindray, Shenzhen, China) is a recently developed hematology analyzer that utilizes fluorescent technology. Based on fluorescent nucleic acid stain and optical detection, the optical platelet counting (PLT-O) on the BC-6000Plus has strong anti-interference potential in platelet (PLT) detection. Its Auto 8×PLT-O Counting Tech can be automatically triggered in low-PLT samples, which enables the PLT-O on the BC-6000Plus to count low PLT more efficiently. Here, we evaluated the performance of the BC-6000Plus automated hematology analyzer in optical PLT counting. METHODS: The basic features (including blank counting, carryover, trueness, and accuracy) of the BC-6000Plus for PLT counting were evaluated according to the Analytical Quality Specifications for Routine Tests in Clinical Hematology (WST 406-2012). Low-PLT samples with a PLT count of below 100×10(9)/L were selected for repeatability tests. Meanwhile, the potential correlations of BC-6000Plus with the XN-L 350 and manual microscopy within different PLT ranges or under interferences of small red blood cells (RBCs) or PLT aggregation were analyzed. RESULTS: The PLT-O on BC-6000Plus met the technical requirements of PLT counting in terms of blank count, carryover, trueness, and accuracy. The repeatability of the enhanced mode (PLT-O 8×) on the BC-6000Plus was better than that of the XN-L 350 in three low PLT count ranges, including 10–20, 20–60, and 60–100 (×10(9)/L). Under the interference-free conditions, the BC-6000Plus correlated well with the XN-L 350 in different PLT counting ranges. Under the interferences of small RBCs and PLT aggregation, the PLT-O on BC-6000Plus correlated better with microscopy than with the platelet impedance count (PLT-I). CONCLUSIONS: The PLT-O on BC-6000Plus can meet the technical requirements of PLT counting in terms of blank counting, carryover rate, trueness, and accuracy. The PLT-O 8× has good repeatability, correlates well with the XN-L 350, and demonstrates good anti-interference ability. It can thus meet the needs of blood cell analysis in clinical settings. AME Publishing Company 2021-03 /pmc/articles/PMC8039664/ /pubmed/33850905 http://dx.doi.org/10.21037/atm-21-691 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Hu, Xuehua Tang, Yuqun Li, Mingyi Fu, Chunyun Deng, Zuoxin Tang, Wenting Zhou, Huideng Chen, Yan Li, Yingfeng Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer |
title | Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer |
title_full | Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer |
title_fullStr | Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer |
title_full_unstemmed | Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer |
title_short | Performance evaluation of optical platelet counting of BC-6000Plus automated hematology analyzer |
title_sort | performance evaluation of optical platelet counting of bc-6000plus automated hematology analyzer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039664/ https://www.ncbi.nlm.nih.gov/pubmed/33850905 http://dx.doi.org/10.21037/atm-21-691 |
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