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First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water

Anisakid nematodes are parasites of cetaceans, their larval stages live in marine fishes. The third-stage larvae of some Anisakis species are also the etiological agents of human anisakiasis caused by consumption of raw or undercooked infected fish. Thus, identification of Anisakis larvae at the spec...

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Autores principales: Van Hien, Hoang, Thi Dung, Bui, Ngo, Ha Duy, Doanh, Pham Ngoc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039996/
https://www.ncbi.nlm.nih.gov/pubmed/33860256
http://dx.doi.org/10.21307/jofnem-2021-010
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author Van Hien, Hoang
Thi Dung, Bui
Ngo, Ha Duy
Doanh, Pham Ngoc
author_facet Van Hien, Hoang
Thi Dung, Bui
Ngo, Ha Duy
Doanh, Pham Ngoc
author_sort Van Hien, Hoang
collection PubMed
description Anisakid nematodes are parasites of cetaceans, their larval stages live in marine fishes. The third-stage larvae of some Anisakis species are also the etiological agents of human anisakiasis caused by consumption of raw or undercooked infected fish. Thus, identification of Anisakis larvae at the species level is crucial for their ecology and epidemiology. In Vietnam, although Anisakis larvae have been reported, they have not been identified to the species level. The aim of this study was, therefore, to identify third-stage larvae of Anisakis collected from marine fishes in Vietnamese water, based on morphological characteristics and molecular analysis. All Anisakis larvae found in this study were morphologically similar to each other and identical to Anisakis typica. In addition, molecular analysis based on ITS1-5.8S-ITS2 sequences confirmed them as A. typica. Vietnamese A. typica population was genetically close to those from Asian countries and Australia. The third-stage larvae of A. typica were collected from eight fish species from three localities in the South of Vietnam. Among them, seven were recorded as new intermediate hosts of A. typica. This is the first identification of A. typica larvae in Vietnamese water with records of new fish hosts.
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spelling pubmed-80399962021-04-14 First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water Van Hien, Hoang Thi Dung, Bui Ngo, Ha Duy Doanh, Pham Ngoc J Nematol Arts & Humanities Anisakid nematodes are parasites of cetaceans, their larval stages live in marine fishes. The third-stage larvae of some Anisakis species are also the etiological agents of human anisakiasis caused by consumption of raw or undercooked infected fish. Thus, identification of Anisakis larvae at the species level is crucial for their ecology and epidemiology. In Vietnam, although Anisakis larvae have been reported, they have not been identified to the species level. The aim of this study was, therefore, to identify third-stage larvae of Anisakis collected from marine fishes in Vietnamese water, based on morphological characteristics and molecular analysis. All Anisakis larvae found in this study were morphologically similar to each other and identical to Anisakis typica. In addition, molecular analysis based on ITS1-5.8S-ITS2 sequences confirmed them as A. typica. Vietnamese A. typica population was genetically close to those from Asian countries and Australia. The third-stage larvae of A. typica were collected from eight fish species from three localities in the South of Vietnam. Among them, seven were recorded as new intermediate hosts of A. typica. This is the first identification of A. typica larvae in Vietnamese water with records of new fish hosts. Exeley Inc. 2021-02-19 /pmc/articles/PMC8039996/ /pubmed/33860256 http://dx.doi.org/10.21307/jofnem-2021-010 Text en © 2021 Authors https://creativecommons.org/licenses/by/4.0/This is an Open Access article licensed under the Creative Commons CC BY 4.0 license, https://creativecommons.org/licenses/by/4.0/
spellingShingle Arts & Humanities
Van Hien, Hoang
Thi Dung, Bui
Ngo, Ha Duy
Doanh, Pham Ngoc
First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
title First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
title_full First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
title_fullStr First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
title_full_unstemmed First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
title_short First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
title_sort first morphological and molecular identification of third-stage larvae of anisakis typica (nematoda: anisakidae) from marine fishes in vietnamese water
topic Arts & Humanities
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039996/
https://www.ncbi.nlm.nih.gov/pubmed/33860256
http://dx.doi.org/10.21307/jofnem-2021-010
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