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CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo

CRISPR-CasRx technology provides a new and powerful method for studying cellular RNA in human cancer. Herein, the pattern of expression of long noncoding RNA 00341 (LINC00341) as well as its biological function in bladder cancer were studied using CRISPR-CasRx. qRT-PCR was employed to quantify the l...

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Autores principales: Li, Chunjing, Cao, Yu, Zhang, Li, Li, Jierong, Wang, Jianfeng, Zhou, Yanfen, Wei, Huiling, Guo, Mingjuan, Liu, Liang, Liu, Chunxiao, Zhang, Shilin, Liu, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040045/
https://www.ncbi.nlm.nih.gov/pubmed/33855047
http://dx.doi.org/10.3389/fmolb.2021.638995
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author Li, Chunjing
Cao, Yu
Zhang, Li
Li, Jierong
Wang, Jianfeng
Zhou, Yanfen
Wei, Huiling
Guo, Mingjuan
Liu, Liang
Liu, Chunxiao
Zhang, Shilin
Liu, Guoqing
author_facet Li, Chunjing
Cao, Yu
Zhang, Li
Li, Jierong
Wang, Jianfeng
Zhou, Yanfen
Wei, Huiling
Guo, Mingjuan
Liu, Liang
Liu, Chunxiao
Zhang, Shilin
Liu, Guoqing
author_sort Li, Chunjing
collection PubMed
description CRISPR-CasRx technology provides a new and powerful method for studying cellular RNA in human cancer. Herein, the pattern of expression of long noncoding RNA 00341 (LINC00341) as well as its biological function in bladder cancer were studied using CRISPR-CasRx. qRT-PCR was employed to quantify the levels of expression of LINC00341 in tumor tissues along with the matched non-tumor tissues. sgRNA targeting LINC00341 or the sgRNA negative control were transiently transfected into the T24 as well as 5,637 human bladder cancer cell lines. CCK-8, ELISA as well as wound healing methods were employed to explore cell proliferation, apoptosis and migration, respectively. The tumorigenicity experiment in nude mice also performed to detect cell proliferation. The expression of p21, Bax as well as E-cadherin were assayed using western blot. The results demonstrated that LINC00341 was overexpressed in bladder cancer in contrast with the healthy tissues. The LINC00341 expression level in high-grade tumors was higher in contrast with that in low-grade tumors. The expression of linc00341 was higher relative to that of non-invasive tumors. In T24 as well as 5637-cell lines harboring LINC00341-sgRNA, inhibition of cell proliferation (in vitro and in vivo), elevated apoptosis rate and diminished migration ability. Moreover, silencing LINC00341 upregulated the expressions of p21, Bax as well as E-cadherin. Knockout of these genes could eliminate the phenotypic changes caused by sgRNA targeting LINC00341. Our data demonstrate that LINC00341 has a carcinogenic role in human bladder cancer.
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spelling pubmed-80400452021-04-13 CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo Li, Chunjing Cao, Yu Zhang, Li Li, Jierong Wang, Jianfeng Zhou, Yanfen Wei, Huiling Guo, Mingjuan Liu, Liang Liu, Chunxiao Zhang, Shilin Liu, Guoqing Front Mol Biosci Molecular Biosciences CRISPR-CasRx technology provides a new and powerful method for studying cellular RNA in human cancer. Herein, the pattern of expression of long noncoding RNA 00341 (LINC00341) as well as its biological function in bladder cancer were studied using CRISPR-CasRx. qRT-PCR was employed to quantify the levels of expression of LINC00341 in tumor tissues along with the matched non-tumor tissues. sgRNA targeting LINC00341 or the sgRNA negative control were transiently transfected into the T24 as well as 5,637 human bladder cancer cell lines. CCK-8, ELISA as well as wound healing methods were employed to explore cell proliferation, apoptosis and migration, respectively. The tumorigenicity experiment in nude mice also performed to detect cell proliferation. The expression of p21, Bax as well as E-cadherin were assayed using western blot. The results demonstrated that LINC00341 was overexpressed in bladder cancer in contrast with the healthy tissues. The LINC00341 expression level in high-grade tumors was higher in contrast with that in low-grade tumors. The expression of linc00341 was higher relative to that of non-invasive tumors. In T24 as well as 5637-cell lines harboring LINC00341-sgRNA, inhibition of cell proliferation (in vitro and in vivo), elevated apoptosis rate and diminished migration ability. Moreover, silencing LINC00341 upregulated the expressions of p21, Bax as well as E-cadherin. Knockout of these genes could eliminate the phenotypic changes caused by sgRNA targeting LINC00341. Our data demonstrate that LINC00341 has a carcinogenic role in human bladder cancer. Frontiers Media S.A. 2021-03-09 /pmc/articles/PMC8040045/ /pubmed/33855047 http://dx.doi.org/10.3389/fmolb.2021.638995 Text en Copyright © 2021 Li, Cao, Zhang, Li, Wang, Zhou, Wei, Guo, Liu, Liu, Zhang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Li, Chunjing
Cao, Yu
Zhang, Li
Li, Jierong
Wang, Jianfeng
Zhou, Yanfen
Wei, Huiling
Guo, Mingjuan
Liu, Liang
Liu, Chunxiao
Zhang, Shilin
Liu, Guoqing
CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo
title CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo
title_full CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo
title_fullStr CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo
title_full_unstemmed CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo
title_short CRISPR-CasRx Targeting LncRNA LINC00341 Inhibits Tumor Cell Growth in vitro and in vivo
title_sort crispr-casrx targeting lncrna linc00341 inhibits tumor cell growth in vitro and in vivo
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040045/
https://www.ncbi.nlm.nih.gov/pubmed/33855047
http://dx.doi.org/10.3389/fmolb.2021.638995
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