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Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm
BACKGROUND: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant “WY16–13” was identified, which exhibited yellow-gr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040211/ https://www.ncbi.nlm.nih.gov/pubmed/33845769 http://dx.doi.org/10.1186/s12864-021-07573-7 |
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author | Nie, Libing Zheng, Yushan Zhang, Liting Wu, Ying Zhu, Shidong Hou, Jinfeng Chen, Guohu Tang, Xiaoyan Wang, Chenggang Yuan, Lingyun |
author_facet | Nie, Libing Zheng, Yushan Zhang, Liting Wu, Ying Zhu, Shidong Hou, Jinfeng Chen, Guohu Tang, Xiaoyan Wang, Chenggang Yuan, Lingyun |
author_sort | Nie, Libing |
collection | PubMed |
description | BACKGROUND: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant “WY16–13” was identified, which exhibited yellow-green leaf color during its entire growth period. However, current understanding of the molecular mechanism underlying Chl metabolism and chloroplast development of “WY16–13” is limited. RESULTS: Total Chl and carotenoid content in WY16–13 was reduced by 60.92 and 58.82%, respectively, as compared with its wild type parental line W16–13. Electron microscopic investigation revealed fewer chloroplasts per cell and looser stroma lamellae in WY16–13 than in W16–13. A comparative transcriptome profiling was performed using leaves from the yellow-green leaf type (WY16–13) and normal green-leaf type (W16–13). A total of 54.12 million (M) (WY16–13) and 56.17 M (W16–13) reads were generated. A total of 40,578 genes were identified from the mapped libraries. We identified 3882 differentially expressed genes (DEGs) in WY16–13 compared with W16–13 (i.e., 1603 upregulated genes and 2279 downregulated genes). According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, these DEGs are involved in porphyrin and Chl metabolism [i.e., chlorophyllase (CLH), heme oxygenase (HO), chlorophyll (ide) b reductase (NYC), and protochlorophyllide oxidoreductase (POR) genes], carbohydrate metabolism, photosynthesis, and carbon fixation in photosynthetic organisms. Moreover, deficiency in Chl biosynthetic intermediates in WY16–13 revealed that the formation of the yellow-green phenotype was related to the disorder of heme metabolism. CONCLUSIONS: Our results provide valuable insights into Chl deficiency in the yellow-green leaf mutant and a bioinformatics resource for further functional identification of key allelic genes responsible for differences in Chl content. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07573-7. |
format | Online Article Text |
id | pubmed-8040211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80402112021-04-12 Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm Nie, Libing Zheng, Yushan Zhang, Liting Wu, Ying Zhu, Shidong Hou, Jinfeng Chen, Guohu Tang, Xiaoyan Wang, Chenggang Yuan, Lingyun BMC Genomics Research Article BACKGROUND: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant “WY16–13” was identified, which exhibited yellow-green leaf color during its entire growth period. However, current understanding of the molecular mechanism underlying Chl metabolism and chloroplast development of “WY16–13” is limited. RESULTS: Total Chl and carotenoid content in WY16–13 was reduced by 60.92 and 58.82%, respectively, as compared with its wild type parental line W16–13. Electron microscopic investigation revealed fewer chloroplasts per cell and looser stroma lamellae in WY16–13 than in W16–13. A comparative transcriptome profiling was performed using leaves from the yellow-green leaf type (WY16–13) and normal green-leaf type (W16–13). A total of 54.12 million (M) (WY16–13) and 56.17 M (W16–13) reads were generated. A total of 40,578 genes were identified from the mapped libraries. We identified 3882 differentially expressed genes (DEGs) in WY16–13 compared with W16–13 (i.e., 1603 upregulated genes and 2279 downregulated genes). According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, these DEGs are involved in porphyrin and Chl metabolism [i.e., chlorophyllase (CLH), heme oxygenase (HO), chlorophyll (ide) b reductase (NYC), and protochlorophyllide oxidoreductase (POR) genes], carbohydrate metabolism, photosynthesis, and carbon fixation in photosynthetic organisms. Moreover, deficiency in Chl biosynthetic intermediates in WY16–13 revealed that the formation of the yellow-green phenotype was related to the disorder of heme metabolism. CONCLUSIONS: Our results provide valuable insights into Chl deficiency in the yellow-green leaf mutant and a bioinformatics resource for further functional identification of key allelic genes responsible for differences in Chl content. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07573-7. BioMed Central 2021-04-12 /pmc/articles/PMC8040211/ /pubmed/33845769 http://dx.doi.org/10.1186/s12864-021-07573-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Nie, Libing Zheng, Yushan Zhang, Liting Wu, Ying Zhu, Shidong Hou, Jinfeng Chen, Guohu Tang, Xiaoyan Wang, Chenggang Yuan, Lingyun Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm |
title | Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm |
title_full | Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm |
title_fullStr | Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm |
title_full_unstemmed | Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm |
title_short | Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm |
title_sort | characterization and transcriptomic analysis of a novel yellow-green leaf wucai (brassica campestris l.) germplasm |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040211/ https://www.ncbi.nlm.nih.gov/pubmed/33845769 http://dx.doi.org/10.1186/s12864-021-07573-7 |
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