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Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway
Background: Gastric carcinoma (GC) is one of the most common malignant tumors and seriously threatens human life and health. Methods: In the present study, 243 differentially expressed proteins in GC were identified using laser capture microdissection (LCM) combined with isotopically labeled quantit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040304/ https://www.ncbi.nlm.nih.gov/pubmed/33867821 http://dx.doi.org/10.7150/ijbs.52591 |
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author | Ye, Dongmei Li, Yuxuan Zhang, Heliang Zhou, Zhiwei Tang, Yujie Wu, Peng Zhao, Qiang Zhang, Zhiwei |
author_facet | Ye, Dongmei Li, Yuxuan Zhang, Heliang Zhou, Zhiwei Tang, Yujie Wu, Peng Zhao, Qiang Zhang, Zhiwei |
author_sort | Ye, Dongmei |
collection | PubMed |
description | Background: Gastric carcinoma (GC) is one of the most common malignant tumors and seriously threatens human life and health. Methods: In the present study, 243 differentially expressed proteins in GC were identified using laser capture microdissection (LCM) combined with isotopically labeled quantitative proteomics technology. The expression of serine protease 1 (PRSS1) protein was analyzed by immunohistochemistry and Western blot. MTT and colony formation assays were employed to determine the effect of PRSS1 expression on the growth and proliferation of GC cells. Then, we observed the expression of miR-146a-5p in GC by qRT-PCR. A dual luciferase assay was performed to determine whether PRSS1 is a target gene of miR-146a-5p. We also explored the influence of miR-146a-5p expression on PRSS1 expression and on the growth and proliferation of GC cells. Finally, Western blotting was used to analyze the effect of PRSS1 expression on the activation of the ERK signaling pathway. Results: We confirmed that PRSS1 expression was significantly increased and was positively correlated with the differentiation, tumor size and lymph node metastasis of GC. Subsequently, we found that overexpression of PRSS1 promoted the growth and proliferation of cells, whereas silencing PRSS1 expression inhibited the growth and proliferation of MGC803 cells by inhibiting activation of the ERK signaling pathway via reductions in PAR-2 activation. MiR-146a-5p targets PRSS1 and suppresses the growth and proliferation of MGC803 cells. Conclusions: miR-146a-5p targets PRSS1 and suppresses the growth and proliferation of MGC803 cells. Silencing PRSS1 expression inhibits the ERK signaling pathway by reducing PAR-2 activation, resulting in suppressed growth and proliferation of MGC803 GC cells. |
format | Online Article Text |
id | pubmed-8040304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-80403042021-04-16 Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway Ye, Dongmei Li, Yuxuan Zhang, Heliang Zhou, Zhiwei Tang, Yujie Wu, Peng Zhao, Qiang Zhang, Zhiwei Int J Biol Sci Research Paper Background: Gastric carcinoma (GC) is one of the most common malignant tumors and seriously threatens human life and health. Methods: In the present study, 243 differentially expressed proteins in GC were identified using laser capture microdissection (LCM) combined with isotopically labeled quantitative proteomics technology. The expression of serine protease 1 (PRSS1) protein was analyzed by immunohistochemistry and Western blot. MTT and colony formation assays were employed to determine the effect of PRSS1 expression on the growth and proliferation of GC cells. Then, we observed the expression of miR-146a-5p in GC by qRT-PCR. A dual luciferase assay was performed to determine whether PRSS1 is a target gene of miR-146a-5p. We also explored the influence of miR-146a-5p expression on PRSS1 expression and on the growth and proliferation of GC cells. Finally, Western blotting was used to analyze the effect of PRSS1 expression on the activation of the ERK signaling pathway. Results: We confirmed that PRSS1 expression was significantly increased and was positively correlated with the differentiation, tumor size and lymph node metastasis of GC. Subsequently, we found that overexpression of PRSS1 promoted the growth and proliferation of cells, whereas silencing PRSS1 expression inhibited the growth and proliferation of MGC803 cells by inhibiting activation of the ERK signaling pathway via reductions in PAR-2 activation. MiR-146a-5p targets PRSS1 and suppresses the growth and proliferation of MGC803 cells. Conclusions: miR-146a-5p targets PRSS1 and suppresses the growth and proliferation of MGC803 cells. Silencing PRSS1 expression inhibits the ERK signaling pathway by reducing PAR-2 activation, resulting in suppressed growth and proliferation of MGC803 GC cells. Ivyspring International Publisher 2021-03-01 /pmc/articles/PMC8040304/ /pubmed/33867821 http://dx.doi.org/10.7150/ijbs.52591 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Ye, Dongmei Li, Yuxuan Zhang, Heliang Zhou, Zhiwei Tang, Yujie Wu, Peng Zhao, Qiang Zhang, Zhiwei Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway |
title | Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway |
title_full | Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway |
title_fullStr | Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway |
title_full_unstemmed | Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway |
title_short | Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway |
title_sort | silencing prss1 suppresses the growth and proliferation of gastric carcinoma cells via the erk pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040304/ https://www.ncbi.nlm.nih.gov/pubmed/33867821 http://dx.doi.org/10.7150/ijbs.52591 |
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