Cargando…
Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers
Due to its specificity, phosphatidylethanol (PEth) 16:0/18:1 has gained increased popularity as a marker for high alcohol consumption in recent years. As conflicting results regarding the stability of PEth 16:0/18:1 in whole blood have been published, there are still uncertainties related to optimum...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040374/ https://www.ncbi.nlm.nih.gov/pubmed/32754728 http://dx.doi.org/10.1093/jat/bkaa082 |
_version_ | 1783677771986239488 |
---|---|
author | Skråstad, Ragnhild Bergene Spigset, Olav Aamo, Trond Oskar Andreassen, Trine Naalsund |
author_facet | Skråstad, Ragnhild Bergene Spigset, Olav Aamo, Trond Oskar Andreassen, Trine Naalsund |
author_sort | Skråstad, Ragnhild Bergene |
collection | PubMed |
description | Due to its specificity, phosphatidylethanol (PEth) 16:0/18:1 has gained increased popularity as a marker for high alcohol consumption in recent years. As conflicting results regarding the stability of PEth 16:0/18:1 in whole blood have been published, there are still uncertainties related to optimum handling, transport and storage of blood samples for the analysis of PEth 16:0/18:1. A stability study where whole blood samples were drawn from healthy volunteers, who had ingested alcohol, is presented. The samples were collected in tubes with ethylenediamine tetraacetic acid (EDTA) and heparin as additives and stored under standardized conditions within 1 h of blood sampling. Storage times were 28 days in ambient temperature and at 4–8°C, and 90 days at −20°C and −80°C. All samples were analyzed regularly during the storage periods. PEth 16:0/18:1 concentrations were stable (defined as < 15% decrease compared with baseline values) at all temperatures up to 28 days, independent of additive. After 90 days of storage at −20°C, the mean concentrations had decreased by 18.8% in EDTA tubes and by 13.8% in heparin tubes. At −80°C, the concentrations were stable throughout the 90-day period. The present study shows that in samples containing PEth formed in vivo, PEth 16:0/18:1 is stable for 28 days irrespective of storage temperature. During long-term storage, samples should be stored at −80°C. |
format | Online Article Text |
id | pubmed-8040374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80403742021-04-15 Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers Skråstad, Ragnhild Bergene Spigset, Olav Aamo, Trond Oskar Andreassen, Trine Naalsund J Anal Toxicol Technical Note Due to its specificity, phosphatidylethanol (PEth) 16:0/18:1 has gained increased popularity as a marker for high alcohol consumption in recent years. As conflicting results regarding the stability of PEth 16:0/18:1 in whole blood have been published, there are still uncertainties related to optimum handling, transport and storage of blood samples for the analysis of PEth 16:0/18:1. A stability study where whole blood samples were drawn from healthy volunteers, who had ingested alcohol, is presented. The samples were collected in tubes with ethylenediamine tetraacetic acid (EDTA) and heparin as additives and stored under standardized conditions within 1 h of blood sampling. Storage times were 28 days in ambient temperature and at 4–8°C, and 90 days at −20°C and −80°C. All samples were analyzed regularly during the storage periods. PEth 16:0/18:1 concentrations were stable (defined as < 15% decrease compared with baseline values) at all temperatures up to 28 days, independent of additive. After 90 days of storage at −20°C, the mean concentrations had decreased by 18.8% in EDTA tubes and by 13.8% in heparin tubes. At −80°C, the concentrations were stable throughout the 90-day period. The present study shows that in samples containing PEth formed in vivo, PEth 16:0/18:1 is stable for 28 days irrespective of storage temperature. During long-term storage, samples should be stored at −80°C. Oxford University Press 2020-07-02 /pmc/articles/PMC8040374/ /pubmed/32754728 http://dx.doi.org/10.1093/jat/bkaa082 Text en © The Author(s) 2020. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Technical Note Skråstad, Ragnhild Bergene Spigset, Olav Aamo, Trond Oskar Andreassen, Trine Naalsund Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers |
title | Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers |
title_full | Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers |
title_fullStr | Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers |
title_full_unstemmed | Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers |
title_short | Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers |
title_sort | stability of phosphatidylethanol 16:0/18:1 in freshly drawn, authentic samples from healthy volunteers |
topic | Technical Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040374/ https://www.ncbi.nlm.nih.gov/pubmed/32754728 http://dx.doi.org/10.1093/jat/bkaa082 |
work_keys_str_mv | AT skrastadragnhildbergene stabilityofphosphatidylethanol160181infreshlydrawnauthenticsamplesfromhealthyvolunteers AT spigsetolav stabilityofphosphatidylethanol160181infreshlydrawnauthenticsamplesfromhealthyvolunteers AT aamotrondoskar stabilityofphosphatidylethanol160181infreshlydrawnauthenticsamplesfromhealthyvolunteers AT andreassentrinenaalsund stabilityofphosphatidylethanol160181infreshlydrawnauthenticsamplesfromhealthyvolunteers |