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CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression

Growing evidence indicates N6-methyladenosine (m6A) has biological function in oncogenesis. METTL3, the catalytic component, is the most important part of methyltransferase complex and plays a crucial role in cancers. However, the biological function of circRNAs derived from METTL3 in breast cancer...

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Autores principales: Li, Zhi, Yang, Hai-Yan, Dai, Xin-Yuan, Zhang, Xu, Huang, Yu-Zhou, Shi, Liang, Wei, Ji-Fu, Ding, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040468/
https://www.ncbi.nlm.nih.gov/pubmed/33867838
http://dx.doi.org/10.7150/ijbs.57783
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author Li, Zhi
Yang, Hai-Yan
Dai, Xin-Yuan
Zhang, Xu
Huang, Yu-Zhou
Shi, Liang
Wei, Ji-Fu
Ding, Qiang
author_facet Li, Zhi
Yang, Hai-Yan
Dai, Xin-Yuan
Zhang, Xu
Huang, Yu-Zhou
Shi, Liang
Wei, Ji-Fu
Ding, Qiang
author_sort Li, Zhi
collection PubMed
description Growing evidence indicates N6-methyladenosine (m6A) has biological function in oncogenesis. METTL3, the catalytic component, is the most important part of methyltransferase complex and plays a crucial role in cancers. However, the biological function of circRNAs derived from METTL3 in breast cancer and the underlying molecular mechanism remains unclear. Herein, we report circMETTL3, which has not been explored in breast cancer, and it is markedly upregulated in breast cancer. Moreover, we uncovered that circMETTL3 could facilitate cell proliferation, migration and invasion in breast cancer. Mechanism investigation showed that circMETTL3 might act as a competing endogenous RNA (ceRNA) of miR-31-5p and upregulate its target cyclin-dependent kinases (CDK1). Moreover, m6A modification of circMETTL3 might affect its expression. Taken together, our results elucidate that circMETTL3 promotes breast cancer progression through circMETTL3/miR-31-5p/CDK1 axis. Moreover, METTL3, the host gene of circMETTL3, may regulate circMETTL3 expression in an m6A-dependent manner, while circMETTL3 has no effect on METTL3 expression, providing a new relationship between the circRNA and the corresponding host gene. Thus, it may serve as a new therapeutic target for breast cancer.
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spelling pubmed-80404682021-04-15 CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression Li, Zhi Yang, Hai-Yan Dai, Xin-Yuan Zhang, Xu Huang, Yu-Zhou Shi, Liang Wei, Ji-Fu Ding, Qiang Int J Biol Sci Research Paper Growing evidence indicates N6-methyladenosine (m6A) has biological function in oncogenesis. METTL3, the catalytic component, is the most important part of methyltransferase complex and plays a crucial role in cancers. However, the biological function of circRNAs derived from METTL3 in breast cancer and the underlying molecular mechanism remains unclear. Herein, we report circMETTL3, which has not been explored in breast cancer, and it is markedly upregulated in breast cancer. Moreover, we uncovered that circMETTL3 could facilitate cell proliferation, migration and invasion in breast cancer. Mechanism investigation showed that circMETTL3 might act as a competing endogenous RNA (ceRNA) of miR-31-5p and upregulate its target cyclin-dependent kinases (CDK1). Moreover, m6A modification of circMETTL3 might affect its expression. Taken together, our results elucidate that circMETTL3 promotes breast cancer progression through circMETTL3/miR-31-5p/CDK1 axis. Moreover, METTL3, the host gene of circMETTL3, may regulate circMETTL3 expression in an m6A-dependent manner, while circMETTL3 has no effect on METTL3 expression, providing a new relationship between the circRNA and the corresponding host gene. Thus, it may serve as a new therapeutic target for breast cancer. Ivyspring International Publisher 2021-03-15 /pmc/articles/PMC8040468/ /pubmed/33867838 http://dx.doi.org/10.7150/ijbs.57783 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Li, Zhi
Yang, Hai-Yan
Dai, Xin-Yuan
Zhang, Xu
Huang, Yu-Zhou
Shi, Liang
Wei, Ji-Fu
Ding, Qiang
CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression
title CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression
title_full CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression
title_fullStr CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression
title_full_unstemmed CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression
title_short CircMETTL3, upregulated in a m6A-dependent manner, promotes breast cancer progression
title_sort circmettl3, upregulated in a m6a-dependent manner, promotes breast cancer progression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040468/
https://www.ncbi.nlm.nih.gov/pubmed/33867838
http://dx.doi.org/10.7150/ijbs.57783
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