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Distribution of microRNAs associated with major depressive disorder among blood compartments

OBJECTIVE: Major depressive disorder (MDD) is a recurrent disorder with an increasing incidence. Alterations in key signaling pathways of the nervous system, such as the Wnt and MAPK pathways, mediated through microRNAs (miRNAs) provide crucial information regarding the etiopathology of MDD. We aime...

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Autores principales: Homorogan, Claudia, Enatescu, Virgil Radu, Nitusca, Diana, Marcu, Anca, Seclaman, Edward, Marian, Catalin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040584/
https://www.ncbi.nlm.nih.gov/pubmed/33827323
http://dx.doi.org/10.1177/03000605211006633
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author Homorogan, Claudia
Enatescu, Virgil Radu
Nitusca, Diana
Marcu, Anca
Seclaman, Edward
Marian, Catalin
author_facet Homorogan, Claudia
Enatescu, Virgil Radu
Nitusca, Diana
Marcu, Anca
Seclaman, Edward
Marian, Catalin
author_sort Homorogan, Claudia
collection PubMed
description OBJECTIVE: Major depressive disorder (MDD) is a recurrent disorder with an increasing incidence. Alterations in key signaling pathways of the nervous system, such as the Wnt and MAPK pathways, mediated through microRNAs (miRNAs) provide crucial information regarding the etiopathology of MDD. We aimed to analyze whether the heterogeneity of literature findings regarding differential expression of miRNAs in the blood could arise from their different distributions among blood compartments. METHODS: We performed a pilot study analyzing the differential expression of miR-26a, miR-494, miR-30c, miR-93, and miR-101 and investigated their levels in white blood cells, total plasma (TP), exosomes from plasma, and exosome depleted plasma (EDP) in patients with MDD before and after antidepressant treatment with escitalopram and in healthy controls. RESULTS: MiR-494 was more abundant in EDP, and miR-26a and miR-30c were predominantly more abundant in TP relative to other blood compartments. Moreover, miR-30c, miR-101, and miR-26a, were significantly downregulated in TP of patients with MDD compared with controls. After antidepressant treatment, only miR-494 was significantly differently expressed in EDP. CONCLUSIONS: This proof-of-principle study suggests that identifying the miRNA abundance in different blood compartments is crucial for biomarker development and could enrich the current knowledge regarding MDD pathophysiology.
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spelling pubmed-80405842021-04-21 Distribution of microRNAs associated with major depressive disorder among blood compartments Homorogan, Claudia Enatescu, Virgil Radu Nitusca, Diana Marcu, Anca Seclaman, Edward Marian, Catalin J Int Med Res Prospective Clinical Research Report OBJECTIVE: Major depressive disorder (MDD) is a recurrent disorder with an increasing incidence. Alterations in key signaling pathways of the nervous system, such as the Wnt and MAPK pathways, mediated through microRNAs (miRNAs) provide crucial information regarding the etiopathology of MDD. We aimed to analyze whether the heterogeneity of literature findings regarding differential expression of miRNAs in the blood could arise from their different distributions among blood compartments. METHODS: We performed a pilot study analyzing the differential expression of miR-26a, miR-494, miR-30c, miR-93, and miR-101 and investigated their levels in white blood cells, total plasma (TP), exosomes from plasma, and exosome depleted plasma (EDP) in patients with MDD before and after antidepressant treatment with escitalopram and in healthy controls. RESULTS: MiR-494 was more abundant in EDP, and miR-26a and miR-30c were predominantly more abundant in TP relative to other blood compartments. Moreover, miR-30c, miR-101, and miR-26a, were significantly downregulated in TP of patients with MDD compared with controls. After antidepressant treatment, only miR-494 was significantly differently expressed in EDP. CONCLUSIONS: This proof-of-principle study suggests that identifying the miRNA abundance in different blood compartments is crucial for biomarker development and could enrich the current knowledge regarding MDD pathophysiology. SAGE Publications 2021-04-07 /pmc/articles/PMC8040584/ /pubmed/33827323 http://dx.doi.org/10.1177/03000605211006633 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Prospective Clinical Research Report
Homorogan, Claudia
Enatescu, Virgil Radu
Nitusca, Diana
Marcu, Anca
Seclaman, Edward
Marian, Catalin
Distribution of microRNAs associated with major depressive disorder among blood compartments
title Distribution of microRNAs associated with major depressive disorder among blood compartments
title_full Distribution of microRNAs associated with major depressive disorder among blood compartments
title_fullStr Distribution of microRNAs associated with major depressive disorder among blood compartments
title_full_unstemmed Distribution of microRNAs associated with major depressive disorder among blood compartments
title_short Distribution of microRNAs associated with major depressive disorder among blood compartments
title_sort distribution of micrornas associated with major depressive disorder among blood compartments
topic Prospective Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040584/
https://www.ncbi.nlm.nih.gov/pubmed/33827323
http://dx.doi.org/10.1177/03000605211006633
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