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Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections

In this review, we focus on the sequenced genomes of the pathogens Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris, and the remarkable discoveries regarding the pathogenicity and genetic information of these organisms, using techniques related to the various omics branches like geno...

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Autores principales: Rodriguez-Anaya, Libia Zulema, Félix-Sastré, Ángel Josué, Lares-Villa, Fernando, Lares-Jiménez, Luis Fernando, Gonzalez-Galaviz, Jose Reyes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040595/
https://www.ncbi.nlm.nih.gov/pubmed/33843581
http://dx.doi.org/10.1051/parasite/2021033
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author Rodriguez-Anaya, Libia Zulema
Félix-Sastré, Ángel Josué
Lares-Villa, Fernando
Lares-Jiménez, Luis Fernando
Gonzalez-Galaviz, Jose Reyes
author_facet Rodriguez-Anaya, Libia Zulema
Félix-Sastré, Ángel Josué
Lares-Villa, Fernando
Lares-Jiménez, Luis Fernando
Gonzalez-Galaviz, Jose Reyes
author_sort Rodriguez-Anaya, Libia Zulema
collection PubMed
description In this review, we focus on the sequenced genomes of the pathogens Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris, and the remarkable discoveries regarding the pathogenicity and genetic information of these organisms, using techniques related to the various omics branches like genomics, transcriptomics, and proteomics. Currently, novel data produced through comparative genomics analyses and both differential gene and protein expression in these free-living amoebas have allowed for breakthroughs to identify genes unique to N. fowleri, genes with active transcriptional activity, and their differential expression in conditions of modified virulence. Furthermore, orthologous genes of the various nuclear genomes within the Naegleria and Acanthamoeba genera have been clustered. The proteome of B. mandrillaris has been reconstructed through transcriptome data, and its mitochondrial genome structure has been thoroughly described with a unique characteristic that has come to light: a type I intron with the capacity of interrupting genes through its self-splicing ribozymes activity. With the integration of data derived from the diverse omic sciences, there is a potential approximation that reflects the molecular complexity required for the identification of virulence factors, as well as crucial information regarding the comprehension of the molecular mechanisms with which these interact. Altogether, these breakthroughs could contribute to radical advances in both the fields of therapy design and medical diagnosis in the foreseeable future.
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spelling pubmed-80405952021-04-13 Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections Rodriguez-Anaya, Libia Zulema Félix-Sastré, Ángel Josué Lares-Villa, Fernando Lares-Jiménez, Luis Fernando Gonzalez-Galaviz, Jose Reyes Parasite Review Article In this review, we focus on the sequenced genomes of the pathogens Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris, and the remarkable discoveries regarding the pathogenicity and genetic information of these organisms, using techniques related to the various omics branches like genomics, transcriptomics, and proteomics. Currently, novel data produced through comparative genomics analyses and both differential gene and protein expression in these free-living amoebas have allowed for breakthroughs to identify genes unique to N. fowleri, genes with active transcriptional activity, and their differential expression in conditions of modified virulence. Furthermore, orthologous genes of the various nuclear genomes within the Naegleria and Acanthamoeba genera have been clustered. The proteome of B. mandrillaris has been reconstructed through transcriptome data, and its mitochondrial genome structure has been thoroughly described with a unique characteristic that has come to light: a type I intron with the capacity of interrupting genes through its self-splicing ribozymes activity. With the integration of data derived from the diverse omic sciences, there is a potential approximation that reflects the molecular complexity required for the identification of virulence factors, as well as crucial information regarding the comprehension of the molecular mechanisms with which these interact. Altogether, these breakthroughs could contribute to radical advances in both the fields of therapy design and medical diagnosis in the foreseeable future. EDP Sciences 2021-04-12 /pmc/articles/PMC8040595/ /pubmed/33843581 http://dx.doi.org/10.1051/parasite/2021033 Text en © L.Z. Rodriguez-Anaya et al., published by EDP Sciences, 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Rodriguez-Anaya, Libia Zulema
Félix-Sastré, Ángel Josué
Lares-Villa, Fernando
Lares-Jiménez, Luis Fernando
Gonzalez-Galaviz, Jose Reyes
Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
title Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
title_full Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
title_fullStr Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
title_full_unstemmed Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
title_short Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
title_sort application of the omics sciences to the study of naegleria fowleri, acanthamoeba spp., and balamuthia mandrillaris: current status and future projections
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040595/
https://www.ncbi.nlm.nih.gov/pubmed/33843581
http://dx.doi.org/10.1051/parasite/2021033
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