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Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology

Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more real...

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Autores principales: Peters, Gregor, Wendler, Olaf, Böhringer, David, Gostian, Antoniu-Oreste, Müller, Sarina K., Canziani, Herbert, Hesse, Nicolas, Semmler, Marion, Berry, David A., Kniesburges, Stefan, Peukert, Wolfgang, Döllinger, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041056/
https://www.ncbi.nlm.nih.gov/pubmed/33850630
http://dx.doi.org/10.3390/app11073011
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author Peters, Gregor
Wendler, Olaf
Böhringer, David
Gostian, Antoniu-Oreste
Müller, Sarina K.
Canziani, Herbert
Hesse, Nicolas
Semmler, Marion
Berry, David A.
Kniesburges, Stefan
Peukert, Wolfgang
Döllinger, Michael
author_facet Peters, Gregor
Wendler, Olaf
Böhringer, David
Gostian, Antoniu-Oreste
Müller, Sarina K.
Canziani, Herbert
Hesse, Nicolas
Semmler, Marion
Berry, David A.
Kniesburges, Stefan
Peukert, Wolfgang
Döllinger, Michael
author_sort Peters, Gregor
collection PubMed
description Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more realistic ex-vivo experiments and promotes a better understanding and improved treatment of dysphonia with regard to mucus consistency. We studied human laryngeal mucus samples from the vocal folds with two complementary approaches: 19 samples were successfully applied to particle tracking microrheology (PTM) and five additional samples to oscillatory shear rheology (OSR). Mucus was collected by experienced laryngologists from patients together with demographic data. The analysis of the viscoelasticity revealed diversity among the investigated mucus samples according to their rigidity (absolute G′ and G″). Moreover some samples revealed throughout solid-like character (G′ > G″), whereas some underwent a change from solid-like to liquid-like (G′ < G″). This led to a subdivision into three groups. We assume that the reason for the differences is a variation in the hydration level of the mucus, which affects the mucin concentration and network formation factors of the mucin mesh. The demographic data could not be correlated to the differences, except for the smoking behavior. Mucus of predominant liquid-like character was associated with current smokers.
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spelling pubmed-80410562021-04-12 Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology Peters, Gregor Wendler, Olaf Böhringer, David Gostian, Antoniu-Oreste Müller, Sarina K. Canziani, Herbert Hesse, Nicolas Semmler, Marion Berry, David A. Kniesburges, Stefan Peukert, Wolfgang Döllinger, Michael Appl Sci (Basel) Article Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more realistic ex-vivo experiments and promotes a better understanding and improved treatment of dysphonia with regard to mucus consistency. We studied human laryngeal mucus samples from the vocal folds with two complementary approaches: 19 samples were successfully applied to particle tracking microrheology (PTM) and five additional samples to oscillatory shear rheology (OSR). Mucus was collected by experienced laryngologists from patients together with demographic data. The analysis of the viscoelasticity revealed diversity among the investigated mucus samples according to their rigidity (absolute G′ and G″). Moreover some samples revealed throughout solid-like character (G′ > G″), whereas some underwent a change from solid-like to liquid-like (G′ < G″). This led to a subdivision into three groups. We assume that the reason for the differences is a variation in the hydration level of the mucus, which affects the mucin concentration and network formation factors of the mucin mesh. The demographic data could not be correlated to the differences, except for the smoking behavior. Mucus of predominant liquid-like character was associated with current smokers. 2021-03-27 2021-04 /pmc/articles/PMC8041056/ /pubmed/33850630 http://dx.doi.org/10.3390/app11073011 Text en https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peters, Gregor
Wendler, Olaf
Böhringer, David
Gostian, Antoniu-Oreste
Müller, Sarina K.
Canziani, Herbert
Hesse, Nicolas
Semmler, Marion
Berry, David A.
Kniesburges, Stefan
Peukert, Wolfgang
Döllinger, Michael
Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
title Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
title_full Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
title_fullStr Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
title_full_unstemmed Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
title_short Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
title_sort human laryngeal mucus from the vocal folds: rheological characterization by particle tracking microrheology and oscillatory shear rheology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041056/
https://www.ncbi.nlm.nih.gov/pubmed/33850630
http://dx.doi.org/10.3390/app11073011
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